LOCUS       sequence-250526-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR G312W.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-G312W
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    247..364
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        412..1590
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          643..1581
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1538..1558
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1610..1634
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1637..1708,1709..3571,3572..3640,3641..5269)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACWADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5271..5295)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5303..5321
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5346..5365)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5347..5365)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5371..5391)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5445..5486
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5507..6074
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5524..6076
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5668..5685)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5895..5914
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6088..6687
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6088..6107)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6584..6604
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6701..7289
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6754..6774)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7172..7183)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7364..7597
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7716..7734)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7802..7906
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7907..7975,7976..8767)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8938..9526
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9427..9446
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9680..9697
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9937..9958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9973..9978,9979..9996,9997..10003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10011..10027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10016..10038
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10035..10051
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10035..10051
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10101..10120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10312..10945
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10992..11117
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11614..11847
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12032..12076
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
       61 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
      121 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
      181 ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg cagtattcat
      241 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
      301 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
      361 ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtcgtg aggctccggt
      421 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      481 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      541 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      601 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      661 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      721 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      781 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      841 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      901 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      961 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     1021 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     1081 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     1141 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     1201 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     1261 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     1321 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1381 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1441 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1501 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1561 agtttttttc ttccatttca ggtgtcgtga agcggccctg cagatatcaa caagtttgta
     1621 caaaaaagca ggcaccatgc gaccctccgg gacggccggg gcagcgctcc tggcgctgct
     1681 ggctgcgctc tgcccggcga gtcgggctct ggaggaaaag aaagtttgcc aaggcacgag
     1741 taacaagctc acgcagttgg gcacttttga agatcatttt ctcagcctcc agaggatgtt
     1801 caataactgt gaggtggtcc ttgggaattt ggaaattacc tatgtgcaga ggaattatga
     1861 tctttccttc ttaaagacca tccaggaggt ggctggttat gtcctcattg ccctcaacac
     1921 agtggagcga attcctttgg aaaacctgca gatcatcaga ggaaatatgt actacgaaaa
     1981 ttcctatgcc ttagcagtct tatctaacta tgatgcaaat aaaaccggac tgaaggagct
     2041 gcccatgaga aatttacagg aaatcctgca tggcgccgtg cggttcagca acaaccctgc
     2101 cctgtgcaac gtggagagca tccagtggcg ggacatagtc agcagtgact ttctcagcaa
     2161 catgtcgatg gacttccaga accacctggg cagctgccaa aagtgtgatc caagctgtcc
     2221 caatgggagc tgctggggtg caggagagga gaactgccag aaactgacca aaatcatctg
     2281 tgcccagcag tgctccgggc gctgccgtgg caagtccccc agtgactgct gccacaacca
     2341 gtgtgctgca ggctgcacag gcccccggga gagcgactgc ctggtctgcc gcaaattccg
     2401 agacgaagcc acgtgcaagg acacctgccc cccactcatg ctctacaacc ccaccacgta
     2461 ccagatggat gtgaaccccg agggcaaata cagctttggt gccacctgcg tgaagaagtg
     2521 tccccgtaat tatgtggtga cagatcacgg ctcgtgcgtc cgagcctgtt gggccgacag
     2581 ctatgagatg gaggaagacg gcgtccgcaa gtgtaagaag tgcgaagggc cttgccgcaa
     2641 agtgtgtaac ggaataggta ttggtgaatt taaagactca ctctccataa atgctacgaa
     2701 tattaaacac ttcaaaaact gcacctccat cagtggcgat ctccacatcc tgccggtggc
     2761 atttaggggt gactccttca cacatactcc tcctctggat ccacaggaac tggatattct
     2821 gaaaaccgta aaggaaatca cagggttttt gctgattcag gcttggcctg aaaacaggac
     2881 ggacctccat gcctttgaga acctagaaat catacgcggc aggaccaagc aacatggtca
     2941 gttttctctt gcagtcgtca gcctgaacat aacatccttg ggattacgct ccctcaagga
     3001 gataagtgat ggagatgtga taatttcagg aaacaaaaat ttgtgctatg caaatacaat
     3061 aaactggaaa aaactgtttg ggacctccgg tcagaaaacc aaaattataa gcaacagagg
     3121 tgaaaacagc tgcaaggcca caggccaggt ctgccatgcc ttgtgctccc ccgagggctg
     3181 ctggggcccg gagcccaggg actgcgtctc ttgccggaat gtcagccgag gcagggaatg
     3241 cgtggacaag tgcaaccttc tggagggtga gccaagggag tttgtggaga actctgagtg
     3301 catacagtgc cacccagagt gcctgcctca ggccatgaac atcacctgca caggacgggg
     3361 accagacaac tgtatccagt gtgcccacta cattgacggc ccccactgcg tcaagacctg
     3421 cccggcagga gtcatgggag aaaacaacac cctggtctgg aagtacgcag acgccggcca
     3481 tgtgtgccac ctgtgccatc caaactgcac ctacggatgc actgggccag gtcttgaagg
     3541 ctgtccaacg aatgggccta agatcccgtc catcgccact gggatggtgg gggccctcct
     3601 cttgctgctg gtggtggccc tggggatcgg cctcttcatg cgaaggcgcc acatcgttcg
     3661 gaagcgcacg ctgcggaggc tgctgcagga gagggagctt gtggagcctc ttacacccag
     3721 tggagaagct cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat
     3781 caaagtgctg ggctccggtg cgttcggcac ggtgtataag ggactctgga tcccagaagg
     3841 tgagaaagtt aaaattcccg tcgctatcaa ggaattaaga gaagcaacat ctccgaaagc
     3901 caacaaggaa atcctcgatg aagcctacgt gatggccagc gtggacaacc cccacgtgtg
     3961 ccgcctgctg ggcatctgcc tcacctccac cgtgcagctc atcacgcagc tcatgccctt
     4021 cggctgcctc ctggactatg tccgggaaca caaagacaat attggctccc agtacctgct
     4081 caactggtgt gtgcagatcg caaagggcat gaactacttg gaggaccgtc gcttggtgca
     4141 ccgcgacctg gcagccagga acgtactggt gaaaacaccg cagcatgtca agatcacaga
     4201 ttttgggctg gccaaactgc tgggtgcgga agagaaagaa taccatgcag aaggaggcaa
     4261 agtgcctatc aagtggatgg cattggaatc aattttacac agaatctata cccaccagag
     4321 tgatgtctgg agctacgggg tgaccgtttg ggagttgatg acctttggat ccaagccata
     4381 tgacggaatc cctgccagcg agatctcctc catcctggag aaaggagaac gcctccctca
     4441 gccacccata tgtaccatcg atgtctacat gatcatggtc aagtgctgga tgatagacgc
     4501 agatagtcgc ccaaagttcc gtgagttgat catcgaattc tccaaaatgg cccgagaccc
     4561 ccagcgctac cttgtcattc agggggatga aagaatgcat ttgccaagtc ctacagactc
     4621 caacttctac cgtgccctga tggatgaaga agacatggac gacgtggtgg atgccgacga
     4681 gtacctcatc ccacagcagg gcttcttcag cagcccctcc acgtcacgga ctcccctcct
     4741 gagctctctg agtgcaacca gcaacaattc caccgtggct tgcattgata gaaatgggct
     4801 gcaaagctgt cccatcaagg aagacagctt cttgcagcga tacagctcag accccacagg
     4861 cgccttgact gaggacagca tagacgacac cttcctccca gtgcctgaat acataaacca
     4921 gtccgttccc aaaaggcccg ctggctctgt gcagaatcct gtctatcaca atcagcctct
     4981 gaaccccgcg cccagcagag acccacacta ccaggacccc cacagcactg cagtgggcaa
     5041 ccccgagtat ctcaacactg tccagcccac ctgtgtcaac agcacattcg acagccctgc
     5101 ccactgggcc cagaaaggca gccaccaaat tagcctggac aaccctgact accagcagga
     5161 cttctttccc aaggaagcca agccaaatgg catctttaag ggctccacag ctgaaaatgc
     5221 agaataccta agggtcgcgc cacaaagcag tgaatttatt ggagcataga acccagcttt
     5281 cttgtacaaa gtggtgatat ccaattaacc ctcactaaag gaaattagtt gaaagtatga
     5341 gaaagcccta tagtgagtcg tattaccacc caacttttct atacaaagtg gttgatatcc
     5401 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
     5461 accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc tccccccccc
     5521 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     5581 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     5641 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     5701 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     5761 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5821 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     5881 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     5941 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     6001 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     6061 aaacacgatg ataatatggc cacacatatg accgagtaca agcccacggt gcgcctcgcc
     6121 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6181 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6241 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6301 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6361 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6421 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6481 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6541 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6601 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6661 tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct aatcaacctc tggattacaa
     6721 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6781 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6841 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6901 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6961 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7021 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7081 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7141 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7201 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7261 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     7321 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7381 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7441 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7501 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7561 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7621 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     7681 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     7741 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7801 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7861 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7921 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7981 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8041 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8101 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     8161 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8221 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8281 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8341 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8401 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8461 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8521 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8581 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8641 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8701 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8761 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8821 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8881 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8941 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9001 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9061 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9121 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9181 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9241 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9301 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9361 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9421 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9481 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9541 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9601 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9661 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9721 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
     9781 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9841 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
     9901 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9961 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    10021 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
    10081 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10141 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    10201 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    10261 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
    10321 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
    10381 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
    10441 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
    10501 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    10561 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
    10621 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
    10681 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
    10741 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
    10801 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10861 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10921 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
    10981 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    11041 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    11101 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    11161 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    11221 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    11281 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    11341 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    11401 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    11461 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    11521 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    11581 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    11641 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    11701 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    11761 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    11821 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    11881 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    11941 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    12001 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    12061 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    12121 at
//
