LOCUS       sequence-250574-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR G983R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-G983R
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    206..323
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        371..1549
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          602..1540
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1497..1517
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1569..1593
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1596..1667,1668..3530,3531..3599,3600..5228)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQRDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5230..5254)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5262..5280
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5305..5324)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5306..5324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5330..5350)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5404..5445
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5466..6033
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5483..6035
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5627..5644)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5854..5873
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6047..6646
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6047..6066)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6543..6563
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6660..7248
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6713..6733)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7131..7142)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7323..7556
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7675..7693)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7761..7865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7866..7934,7935..8726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8897..9485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9386..9405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9639..9656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9896..9917
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9932..9937,9938..9955,9956..9962)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9970..9986
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9975..9997
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9994..10010
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9994..10010
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10060..10079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10271..10904
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10951..11076
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11573..11806
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11991..12035
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
       61 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
      121 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt
      181 cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
      241 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
      301 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
      361 ctagtcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
      421 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
      481 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
      541 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
      601 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
      661 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
      721 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
      781 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
      841 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
      901 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
      961 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     1021 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     1081 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     1141 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     1201 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     1261 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     1321 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     1381 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     1441 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     1501 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc
     1561 agatatcaac aagtttgtac aaaaaagcag gcaccatgcg accctccggg acggccgggg
     1621 cagcgctcct ggcgctgctg gctgcgctct gcccggcgag tcgggctctg gaggaaaaga
     1681 aagtttgcca aggcacgagt aacaagctca cgcagttggg cacttttgaa gatcattttc
     1741 tcagcctcca gaggatgttc aataactgtg aggtggtcct tgggaatttg gaaattacct
     1801 atgtgcagag gaattatgat ctttccttct taaagaccat ccaggaggtg gctggttatg
     1861 tcctcattgc cctcaacaca gtggagcgaa ttcctttgga aaacctgcag atcatcagag
     1921 gaaatatgta ctacgaaaat tcctatgcct tagcagtctt atctaactat gatgcaaata
     1981 aaaccggact gaaggagctg cccatgagaa atttacagga aatcctgcat ggcgccgtgc
     2041 ggttcagcaa caaccctgcc ctgtgcaacg tggagagcat ccagtggcgg gacatagtca
     2101 gcagtgactt tctcagcaac atgtcgatgg acttccagaa ccacctgggc agctgccaaa
     2161 agtgtgatcc aagctgtccc aatgggagct gctggggtgc aggagaggag aactgccaga
     2221 aactgaccaa aatcatctgt gcccagcagt gctccgggcg ctgccgtggc aagtccccca
     2281 gtgactgctg ccacaaccag tgtgctgcag gctgcacagg cccccgggag agcgactgcc
     2341 tggtctgccg caaattccga gacgaagcca cgtgcaagga cacctgcccc ccactcatgc
     2401 tctacaaccc caccacgtac cagatggatg tgaaccccga gggcaaatac agctttggtg
     2461 ccacctgcgt gaagaagtgt ccccgtaatt atgtggtgac agatcacggc tcgtgcgtcc
     2521 gagcctgtgg ggccgacagc tatgagatgg aggaagacgg cgtccgcaag tgtaagaagt
     2581 gcgaagggcc ttgccgcaaa gtgtgtaacg gaataggtat tggtgaattt aaagactcac
     2641 tctccataaa tgctacgaat attaaacact tcaaaaactg cacctccatc agtggcgatc
     2701 tccacatcct gccggtggca tttaggggtg actccttcac acatactcct cctctggatc
     2761 cacaggaact ggatattctg aaaaccgtaa aggaaatcac agggtttttg ctgattcagg
     2821 cttggcctga aaacaggacg gacctccatg cctttgagaa cctagaaatc atacgcggca
     2881 ggaccaagca acatggtcag ttttctcttg cagtcgtcag cctgaacata acatccttgg
     2941 gattacgctc cctcaaggag ataagtgatg gagatgtgat aatttcagga aacaaaaatt
     3001 tgtgctatgc aaatacaata aactggaaaa aactgtttgg gacctccggt cagaaaacca
     3061 aaattataag caacagaggt gaaaacagct gcaaggccac aggccaggtc tgccatgcct
     3121 tgtgctcccc cgagggctgc tggggcccgg agcccaggga ctgcgtctct tgccggaatg
     3181 tcagccgagg cagggaatgc gtggacaagt gcaaccttct ggagggtgag ccaagggagt
     3241 ttgtggagaa ctctgagtgc atacagtgcc acccagagtg cctgcctcag gccatgaaca
     3301 tcacctgcac aggacgggga ccagacaact gtatccagtg tgcccactac attgacggcc
     3361 cccactgcgt caagacctgc ccggcaggag tcatgggaga aaacaacacc ctggtctgga
     3421 agtacgcaga cgccggccat gtgtgccacc tgtgccatcc aaactgcacc tacggatgca
     3481 ctgggccagg tcttgaaggc tgtccaacga atgggcctaa gatcccgtcc atcgccactg
     3541 ggatggtggg ggccctcctc ttgctgctgg tggtggccct ggggatcggc ctcttcatgc
     3601 gaaggcgcca catcgttcgg aagcgcacgc tgcggaggct gctgcaggag agggagcttg
     3661 tggagcctct tacacccagt ggagaagctc ccaaccaagc tctcttgagg atcttgaagg
     3721 aaactgaatt caaaaagatc aaagtgctgg gctccggtgc gttcggcacg gtgtataagg
     3781 gactctggat cccagaaggt gagaaagtta aaattcccgt cgctatcaag gaattaagag
     3841 aagcaacatc tccgaaagcc aacaaggaaa tcctcgatga agcctacgtg atggccagcg
     3901 tggacaaccc ccacgtgtgc cgcctgctgg gcatctgcct cacctccacc gtgcagctca
     3961 tcacgcagct catgcccttc ggctgcctcc tggactatgt ccgggaacac aaagacaata
     4021 ttggctccca gtacctgctc aactggtgtg tgcagatcgc aaagggcatg aactacttgg
     4081 aggaccgtcg cttggtgcac cgcgacctgg cagccaggaa cgtactggtg aaaacaccgc
     4141 agcatgtcaa gatcacagat tttgggctgg ccaaactgct gggtgcggaa gagaaagaat
     4201 accatgcaga aggaggcaaa gtgcctatca agtggatggc attggaatca attttacaca
     4261 gaatctatac ccaccagagt gatgtctgga gctacggggt gaccgtttgg gagttgatga
     4321 cctttggatc caagccatat gacggaatcc ctgccagcga gatctcctcc atcctggaga
     4381 aaggagaacg cctccctcag ccacccatat gtaccatcga tgtctacatg atcatggtca
     4441 agtgctggat gatagacgca gatagtcgcc caaagttccg tgagttgatc atcgaattct
     4501 ccaaaatggc ccgagacccc cagcgctacc ttgtcattca gagggatgaa agaatgcatt
     4561 tgccaagtcc tacagactcc aacttctacc gtgccctgat ggatgaagaa gacatggacg
     4621 acgtggtgga tgccgacgag tacctcatcc cacagcaggg cttcttcagc agcccctcca
     4681 cgtcacggac tcccctcctg agctctctga gtgcaaccag caacaattcc accgtggctt
     4741 gcattgatag aaatgggctg caaagctgtc ccatcaagga agacagcttc ttgcagcgat
     4801 acagctcaga ccccacaggc gccttgactg aggacagcat agacgacacc ttcctcccag
     4861 tgcctgaata cataaaccag tccgttccca aaaggcccgc tggctctgtg cagaatcctg
     4921 tctatcacaa tcagcctctg aaccccgcgc ccagcagaga cccacactac caggaccccc
     4981 acagcactgc agtgggcaac cccgagtatc tcaacactgt ccagcccacc tgtgtcaaca
     5041 gcacattcga cagccctgcc cactgggccc agaaaggcag ccaccaaatt agcctggaca
     5101 accctgacta ccagcaggac ttctttccca aggaagccaa gccaaatggc atctttaagg
     5161 gctccacagc tgaaaatgca gaatacctaa gggtcgcgcc acaaagcagt gaatttattg
     5221 gagcatagaa cccagctttc ttgtacaaag tggtgatatc caattaaccc tcactaaagg
     5281 tgtaagttta aagtaagaaa tgtgccctat agtgagtcgt attaccaccc aacttttcta
     5341 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
     5401 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa
     5461 tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     5521 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     5581 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     5641 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     5701 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     5761 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5821 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5881 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     5941 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     6001 gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatga ccgagtacaa
     6061 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6121 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6181 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6241 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6301 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6361 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6421 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6481 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6541 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6601 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctaaatcg atagatccta
     6661 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6721 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6781 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6841 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6901 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6961 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     7021 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     7081 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     7141 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     7201 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt
     7261 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7321 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     7381 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7441 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7501 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7561 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7621 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     7681 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8101 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8401 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9541 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9601 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9661 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9721 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9781 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9841 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9901 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9961 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
    10021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10081 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    10141 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    10201 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    10261 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
    10321 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
    10381 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
    10441 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
    10501 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
    10561 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
    10621 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
    10681 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
    10741 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    10801 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    10861 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
    10921 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
    10981 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
    11041 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
    11101 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
    11161 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
    11221 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
    11281 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
    11341 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
    11401 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
    11461 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
    11521 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
    11581 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
    11641 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
    11701 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    11761 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    11821 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    11881 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    11941 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    12001 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    12061 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    12121 tt
//
