LOCUS       sequence-250634-       10788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF H585Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-H585Y
FEATURES             Location/Qualifiers
     source          1..10788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1389..1398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3693..3717)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3725..3743
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3768..3787)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3769..3787)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3793..3813)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3867..3908
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3929..4494
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3944..4496
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4088..4105)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4315..4334
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4508..5107
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4508..4527)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5004..5024
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5121..5709
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5174..5194)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5592..5603)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5784..6017
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6136..6154)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6222..6326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6327..6395,6396..7187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6545..6564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7358..7946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7847..7866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8100..8117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8357..8378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8393..8398,8399..8416,8417..8423)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8431..8447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8436..8458
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8455..8471
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8455..8471
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8521..8540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8732..9365
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9412..9537
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10034..10267
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10452..10496
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atggcggcgc tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc
     1441 tctgttcaac ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggcctcttc
     1501 ggctgcggac cctgccattc cggaggaggt gtggaatatc aaacaaatga ttaagttgac
     1561 acaggaacat atagaggccc tattggacaa atttggtggg gagcataatc caccatcaat
     1621 atatctggag gcctatgaag aatacaccag caagctagat gcactccaac aaagagaaca
     1681 acagttattg gaatctctgg ggaacggaac tgatttttct gtttctagct ctgcatcaat
     1741 ggataccgtt acatcttctt cctcttctag cctttcagtg ctaccttcat ctctttcagt
     1801 ttttcaaaat cccacagatg tggcacggag caaccccaag tcaccacaaa aacctatcgt
     1861 tagagtcttc ctgcccaaca aacagaggac agtggtacct gcaaggtgtg gagttacagt
     1921 ccgagacagt ctaaagaaag cactgatgat gagaggtcta atcccagagt gctgtgctgt
     1981 ttacagaatt caggatggag agaagaaacc aattggttgg gacactgata tttcctggct
     2041 tactggagaa gaattgcatg tggaagtgtt ggagaatgtt ccacttacaa cacacaactt
     2101 tgtacgaaaa acgtttttca ccttagcatt ttgtgacttt tgtcgaaagc tgcttttcca
     2161 gggtttccgc tgtcaaacat gtggttataa atttcaccag cgttgtagta cagaagttcc
     2221 actgatgtgt gttaattatg accaacttga tttgctgttt gtctccaagt tctttgaaca
     2281 ccacccaata ccacaggaag aggcgtcctt agcagagact gccctaacat ctggatcatc
     2341 cccttccgca cccgcctcgg actctattgg gccccaaatt ctcaccagtc cgtctccttc
     2401 aaaatccatt ccaattccac agcccttccg accagcagat gaagatcatc gaaatcaatt
     2461 tgggcaacga gaccgatcct catcagctcc caatgtgcat ataaacacaa tagaacctgt
     2521 caatattgat gacttgatta gagaccaagg atttcgtggt gatggaggat caaccacagg
     2581 tttgtctgct accccccctg cctcattacc tggctcacta actaacgtga aagccttaca
     2641 gaaatctcca ggacctcagc gagaaaggaa gtcatcttca tcctcagaag acaggaatcg
     2701 aatgaaaaca cttggtagac gggactcgag tgatgattgg gagattcctg atgggcagat
     2761 tacagtggga caaagaattg gatctggatc atttggaaca gtctacaagg gaaagtggca
     2821 tggtgatgtg gcagtgaaaa tgttgaatgt gacagcacct acacctcagc agttacaagc
     2881 cttcaaaaat gaagtaggag tactcaggaa aacacgacat gtgaatatcc tactcttcat
     2941 gggctattcc acaaagccac aactggctat tgttacccag tggtgtgagg gctccagctt
     3001 gtatcaccat ctccatatca ttgagaccaa atttgagatg atcaaactta tagatattgc
     3061 acgacagact gcacagggca tggattactt acacgccaag tcaatcatcc acagagacct
     3121 caagagtaat aatatatttc tttatgaaga cctcacagta aaaataggtg attttggtct
     3181 agctacagtg aaatctcgat ggagtgggtc ccatcagttt gaacagttgt ctggatccat
     3241 tttgtggatg gcaccagaag tcatcagaat gcaagataaa aatccataca gctttcagtc
     3301 agatgtatat gcatttggaa ttgttctgta tgaattgatg actggacagt taccttattc
     3361 aaacatcaac aacagggacc agataatttt tatggtggga cgaggatacc tgtctccaga
     3421 tctcagtaag gtacggagta actgtccaaa agccatgaag agattaatgg cagagtgcct
     3481 caaaaagaaa agagatgaga gaccactctt tccccaaatt ctcgcctcta ttgagctgct
     3541 ggcccgctca ttgccaaaaa ttcaccgcag tgcatcagaa ccctccttga atcgggctgg
     3601 tttccaaaca gaggatttta gtctatatgc ttgtgcttct ccaaaaacac ccatccaggc
     3661 agggggatat ggtgcgtttc ctgtccacta gaacccagct ttcttgtaca aagtggtgat
     3721 atccaattaa ccctcactaa aggaagatga tagttaagtg taagttaccc tatagtgagt
     3781 cgtattacca cccaactttt ctatacaaag tggttgatat ccagcacagt ggcggccgct
     3841 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     3901 attctacgcg taccggttag taatgagatc cctccccccc ctaacgttac tggccgaagc
     3961 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     4021 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     4081 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     4141 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     4201 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     4261 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     4321 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     4381 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     4441 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     4501 cacacatatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     4561 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     4621 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     4681 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     4741 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     4801 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     4861 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     4921 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     4981 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5041 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5101 tgcctaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     5161 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5221 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5281 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5341 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5401 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5461 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5521 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5581 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5641 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5701 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5761 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5821 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5881 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5941 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6001 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6061 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     6121 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6181 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6541 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     6601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6841 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6901 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6961 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7021 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7081 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7141 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7201 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7501 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8041 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8101 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8161 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
     8221 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8281 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
     8341 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8401 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8461 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
     8521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     8581 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
     8641 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
     8701 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
     8761 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     8821 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     8881 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     8941 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     9001 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     9061 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     9121 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     9181 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9241 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9301 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9361 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     9421 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     9481 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     9541 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9601 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9661 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9721 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9781 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9841 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     9901 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     9961 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10021 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10081 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10141 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10201 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10261 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    10321 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    10381 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    10441 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    10501 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    10561 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    10621 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
    10681 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
    10741 gatctcgacg gtatcgccga attcacaaat ggcagtattc atccacaa
//
