LOCUS       sequence-250698-       10282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PPP2R1A P179R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PPP2R1A-P179R
FEATURES             Location/Qualifiers
     source          1..10282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3446..3455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5219..5243)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5296..5337
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5358..5925
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5375..5927
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5519..5536)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5746..5765
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5939..5941,5942..5944,5945..6658)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5939..5941,5942..5944,5945..6655)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5984..6005)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6245..6264)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6592..6613
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6672..7260
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6725..6745)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7143..7154)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7335..7568
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7687..7705)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7773..7877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7878..7946,7947..8738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8096..8115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8909..9497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9398..9417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9651..9668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9908..9929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9944..9949,9950..9967,9968..9974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9982..9998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9987..10009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10006..10022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10006..10022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10072..10091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gcggcggccg acggcgacga ctcgctgtac
     3481 cccatcgcgg tgctcataga cgaactccgc aatgaggacg ttcagcttcg cctcaacagc
     3541 atcaagaagc tgtccaccat cgccttggcc cttggggttg aaaggacccg aagtgagctt
     3601 ctgcctttcc ttacagatac catctatgat gaagatgagg tcctcctggc cctggcagaa
     3661 cagctgggaa ccttcactac cctggtggga ggcccagagt acgtgcactg cctgctgcca
     3721 ccgctggagt cgctggccac agtggaggag acagtggtgc gggacaaggc agtggagtcc
     3781 ttacgggcca tctcacacga gcactcgccc tctgacctgg aggcgcactt tgtgccgcta
     3841 gtgaagcggc tggcgggcgg cgactggttc acctcccgca cctcggcctg cggcctcttc
     3901 tccgtctgct acccccgagt gtccagtgct gtgaaggcgg aacttcgaca gtacttccgg
     3961 aacctgtgct cagatgacac ccgcatggtg cggcgggccg cagcctccaa gctgggggag
     4021 tttgccaagg tgctggagct ggacaacgtc aagagtgaga tcatccccat gttctccaac
     4081 ctggcctctg acgagcagga ctcggtgcgg ctgctggcgg tggaggcgtg cgtgaacatc
     4141 gcccagcttc tgccccagga ggatctggag gccctggtga tgcccactct gcgccaggcc
     4201 gctgaagaca agtcctggcg cgtccgctac atggtggctg acaagttcac agagctccag
     4261 aaagcagtgg ggcctgagat caccaagaca gacctggtcc ctgccttcca gaacctgatg
     4321 aaagactgtg aggccgaggt gagggccgca gcctcccaca aggtcaaaga gttctgtgaa
     4381 aacctctcag ctgactgtcg ggagaatgtg atcatgtccc agatcttgcc ctgcatcaag
     4441 gagctggtgt ccgatgccaa ccaacatgtc aagtctgccc tggcctcagt catcatgggt
     4501 ctctctccca tcttgggcaa agacaacacc atcgagcacc tcttgcccct cttcctggct
     4561 cagctgaagg atgagtgccc tgaggtacgg ctgaacatca tctctaacct ggactgtgtg
     4621 aacgaggtga ttggcatccg gcagctgtcc cagtccctgc tccctgccat tgtggagctg
     4681 gctgaggacg ccaagtggcg ggtgcggctg gccatcattg agtacatgcc cctcctggct
     4741 ggacagctgg gagtggagtt ctttgatgag aaacttaact ccttgtgcat ggcctggctt
     4801 gtggatcatg tatatgccat ccgcgaggca gccaccagca acctgaagaa gctagtggaa
     4861 aagtttggga aggagtgggc ccatgccaca atcatcccca aggtcttggc catgtccgga
     4921 gaccccaact acctgcaccg catgactacg ctcttctgca tcaatgtgct gtctgaggtc
     4981 tgtgggcagg acatcaccac caagcacatg ctacccacgg ttctgcgcat ggctggggac
     5041 ccggttgcca atgtccgctt caatgtggcc aagtctctgc agaagatagg gcccatcctg
     5101 gacaacagca ccttgcagag tgaagtcaag cccatcctag agaagctgac ccaggaccag
     5161 gatgtggacg tcaaatactt tgcccaggag gctctgactg ttctgtctct cgcctagaac
     5221 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag
     5281 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     5341 accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     5401 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5461 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5521 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5581 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5641 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     5701 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     5761 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     5821 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     5881 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat
     5941 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     6001 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     6061 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     6121 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     6181 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     6241 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     6301 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     6361 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     6421 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     6481 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     6541 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     6601 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaat
     6661 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6721 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6781 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6841 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6901 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6961 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7021 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7081 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7141 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7201 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7261 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7321 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7381 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7441 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7501 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7561 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7621 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac
     7681 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7741 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7801 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7861 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7921 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7981 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8041 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8101 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     8161 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8221 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8281 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8341 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8401 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8461 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8521 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8581 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8641 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8701 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8761 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8821 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8881 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8941 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9001 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9061 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9121 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9181 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9241 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9301 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9361 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9421 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9481 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9541 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9601 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9661 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9721 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc
     9781 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9841 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    10021 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10081 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    10141 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    10201 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10261 tatgtactac aaacttagta gt
//
