LOCUS       sequence-250741-        2284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part. TSS promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.18.01401"; journalName: "Plant Physiol"; date: 
            "2019-05"; volume: "180"; issue: "1"; pages: "39-55"
COMMENT     Sequence Label: pC0.203
FEATURES             Location/Qualifiers
     source          1..2284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     155..172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        253..287
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     promoter        253..287
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     primer_bind     complement(323..341)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        409..513
                     /gene="bla"
                     /label=AmpR promoter
     CDS             735..1526
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(1697..2284,1)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2186..2205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg
      241 tggtctctgg agttgacagc tagctcagtc ctagggatta tgctagcaga gaccacgaag
      301 tggctcttca gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      361 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      421 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      481 taaatgcttc aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc
      541 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
      601 ttttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
      661 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
      721 caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
      781 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
      841 ccgcagtgga tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg
      901 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
      961 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     1021 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     1081 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     1141 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     1201 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     1261 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     1321 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     1381 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     1441 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtaaaaggcg
     1501 agatcaccaa ggtagtcggc aaataactgt cagaccaagt ttactcatat atactttaga
     1561 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1621 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1681 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1741 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1801 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1861 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1921 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1981 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2041 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2101 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2161 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2221 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc ttcagggggg cggagcctat
     2281 ggaa
//
