LOCUS       sequence-25078-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  TITLE     Leder Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MMTV-TGF-beta-1
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..41
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    38..172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(62..81)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     116..135
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(444..464)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          594..659
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 acngactcnc gcggnggcgg ccgctctaga actagtggat ccagacatga taagatacat
       61 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
      121 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
      181 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
      241 gtaaaacctc tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc
      301 aaatattttt ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata
      361 gcaaagcaag caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct
      421 gaaggaaagt ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga
      481 gagtcagcag tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc
      541 tcattaaagg cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat
      601 acacaaacaa ttagaatcag tagtttaaca cattatacac ttnaaaattt tatanttacc
      661 ttagagcttt catcccctgn aggtagtttg tccaattang tcacaccaca gaagtaaggt
      721 tccttcccac anancctccc anantcgcgg cccgccacct gcaggaattc nggcccctcn
      781 nccacctntn a
//
