LOCUS       sequence-250791-        5890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized Integrase 2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINT2
FEATURES             Location/Qualifiers
     source          1..5890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        86..1297
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1134..1153
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2951..2970)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3016..3071
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3070..3089)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3432..3448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3432..3448)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3445..3467)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3456..3472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3480..3486,3487..3504,3505..3510))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3525..3546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3605..3800
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3651..3786
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3806..3940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3843..3862)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3897..3916
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4008..4025)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4179..4767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4259..4278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4938..5729,5730..5798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5561..5580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(5799..5890,1..13))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact
       61 agtaacccgt gtcggctcca gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc
      121 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
      181 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
      241 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
      301 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
      361 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
      421 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
      481 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
      541 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
      601 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
      661 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
      721 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
      781 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
      841 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
      901 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
      961 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     1021 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     1081 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     1141 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     1201 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     1261 cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg caggtcgagc
     1321 tcaagcttcg aattcatgcc catcgccccg gagttcctga gtttggccta tccgggacaa
     1381 gagtttcctg cttatctcta cgggagggct tccagggatc cgaagagaaa aggtcgaagc
     1441 gtccaaagcc aattggacga agggagggca acctgtcttg atgctggatg gcctattgct
     1501 ggggaattta aagatgtaga cagatcagca tctgcctatg ctaggaggac gcgagatgaa
     1561 tttgaagaga tgatcgcagg aatccaggcg ggggagtgcc ggattcttgt ggctttcgaa
     1621 gcgtctcgat attacagaga tttggaggcc tatgttcggc ttcggcgagt ctgccgggaa
     1681 gcaggcgttc ttctttgcta taacggtcag gtgtacgatc ttagtaagag tgcagataga
     1741 aaggcgacgg ctcaggatgc ggtgaacgca gaaggcgaag ccgacgatat aagagaaaga
     1801 aacctccgca ccaccagact taatgccaag cgagggggag ctcatgggcc tgtcccagat
     1861 ggatataaga gacgatatga tccagattcc ggagatcttg tcgaccaaat cccgcacccc
     1921 gacagagccg gtttgataac tgagattttt aggcgggcag cggcggccga gccactggcc
     1981 gctatctgcc gagatctcaa tgagaggggc gaaactaccc accgaggcaa agcatggcag
     2041 aggcaccacc tgcatgcgat ccttcgcaat ccagcttata tcggtcatag aagacatctc
     2101 ggtgttgaca caggtaaagg aatgtgggca cctatttgcg atgatgagga ctttgcggaa
     2161 acctttcagg ctgtccagga aatcctcagt cttccaggga gacagttgag cccgggccct
     2221 gaagcgcaac atttgcagac agggatcgca ctttgcggag aacacccgga cgaaccgccg
     2281 cttaggagtg tgactgttcg aggccgcaca aattacaatt gttccacaag atacgatgtg
     2341 gcgatgcgag aagaccgcat ggatgcgttc gtcgaggagt ccgtcatcac gtggctggcc
     2401 tccgacgaag cagttgcagc ttttgaagat aatactgacg atgaacggac aagaaaagcg
     2461 aggatcagac tcaaagttct tgaagagcag ctcgaggctg cacaaaaaca ggctagaaca
     2521 ctccgcccgg acggaatggg catgctcctt agtatagatt ccttggccgg cttggaagcc
     2581 gaacttactc cacagataga caaggcgagg caggagagtc ggtctttgca cgtacccgct
     2641 ctcttgagag acctgttggg caaaccgcga gctgatgttg accgcgcatg gaacgaagca
     2701 ctcacgttgc cccagcgccg aatgatactg cgcatggttg tcactatccg cctcttcaaa
     2761 gcaggatctc gaggtgtacg ggcgatagag ccaggtcgaa ttaccttgag ttatgttgga
     2821 gaaccaggat ttaagcccgt gggcggaaat cgagcaaaac agtaagcggc cgcactcctc
     2881 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     2941 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     3001 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     3061 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     3121 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     3181 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     3241 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     3301 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     3361 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     3421 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     3601 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3661 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3721 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3781 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     3841 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3901 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     3961 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4021 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4081 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4141 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4201 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4261 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4321 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4381 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4441 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4501 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4561 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4621 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4681 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4741 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4801 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4861 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4921 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4981 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5041 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5101 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5161 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5221 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5281 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5341 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5401 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5461 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5521 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5581 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5641 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5701 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5761 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5821 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5881 aataaacaaa
//
