LOCUS       sequence-250811-        1473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of CD63-pHluorin for visualization of multivesicular 
            body-plasma membrane fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Verweij FJ, Bebelman MP, Jimenez CR, Garcia-Vallejo JJ, Janssen H, 
            Neefjes J, Knol JC, de Goeij-de Haas R, Piersma SR, Baglio SR, 
            Verhage M, Middeldorp JM, Zomer A, van Rheenen J, Coppolino MG, 
            Hurbain I, Raposo G, Smit MJ, Toonen RFG, van Niel G, Pegtel DM
  TITLE     Quantifying exosome secretion from single cells reveals a modulatory
            role for GPCR signaling.
  JOURNAL   J Cell Biol. 2018 Mar 5;217(3):1129-1142. doi: 
            10.1083/jcb.201703206. Epub 2018 Jan 16.
  PUBMED    29339438
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201703206"; journalName: "J Cell Biol"; date: 
            "2018-03-5- 5"; volume: "217"; issue: "3"; pages: "1129-1142"
COMMENT     Sequence Label: pCMV-Sport6-CD63-pHluorin
FEATURES             Location/Qualifiers
     source          1..1473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             124..840
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(157..185)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     780..800
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atggcggtgg aaggaggaat gaaatgtgtg aagttcttgc tctacgtcct cctgctggcc
       61 ttttgcgcct gtgcagtggg actgattgcc gtgggtgtcg gggcacagag atctctagcc
      121 accatgggaa gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      181 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      241 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccttggcca
      301 acacttgtca ctactttaac ttatggtgtt caatgctttt caagataccc agatcatatg
      361 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      421 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      481 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      541 cacaaattgg aatacaacta taacgatcac caggtgtaca tcatggcaga caaacaaaag
      601 aatggaatca aagctaactt caaaattaga cacaacattg aagatggagg cgttcaacta
      661 gcagaccatt atcaacaaaa tactccaatt ggcgatgggc ccgtcctttt accagacaac
      721 cattacctgt ttacaacttc tactctttcg aaagatccca acgaaaagag agaccacatg
      781 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      841 accggtggag ctcgaatcag atctcttgtc ctgagtcaga ccataatcca gggggctacc
      901 cctggctctc tgttgccagt ggtcatcatc gcagtgggtg tcttcctctt cctggtggct
      961 tttgtgggct gctgcggggc ctgcaaggag aactattgtc ttatgatcac gtttgccatc
     1021 tttctgtctc ttatcatgtt ggtggaggtg gccgcagcca ttgctggcta tgtgtttaga
     1081 gataaggtga tgtcagagtt taataacaac ttccggcagc agatggagaa ttacccgaaa
     1141 aacaaccaca ctgcttcgat cctggacagg atgcaggcag attttaagtg ctgtggggct
     1201 gctaactaca cagattggga gaaaatccct tccatgtcga agaaccgagt ccccgactcc
     1261 tgctgcatta atgttactgt gggctgtggg attaatttca acgagaaggc gatccataag
     1321 gagggctgtg tggagaagat tgggggctgg ctgaggaaaa atgtgctggt ggtagctgca
     1381 gcagcccttg gaattgcttt tgtcgaggtt ttgggaattg tctttgcctg ctgcctcgtg
     1441 aagagtatca gaagtggcta cgaggtgatg tag
//
