LOCUS       sequence-250823-        5785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes H. sapiens codon optimized Integrase 13..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, 
            Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima 
            RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, 
            Rech E
  TITLE     Genetic switches designed for eukaryotic cells and controlled by 
            serine integrases.
  JOURNAL   Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.
  PUBMED    32444777
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-22"; volume: "3(1):255. doi"; pages: " 
            10.1038/s42003-020-0971-8"
COMMENT     Sequence Label: INCTbiosyn-pUB-HspINT13
FEATURES             Location/Qualifiers
     source          1..5785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..1272
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1109..1128
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2821..2840)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2886..2941
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2940..2959)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3302..3318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3302..3318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3315..3337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3326..3342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3350..3356,3357..3374,3375..3380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3395..3416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3475..3670
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3521..3656
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3676..3810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3713..3732)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3767..3786
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3878..3895)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4049..4637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4129..4148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4808..5599,5600..5668))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5431..5450
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5669..5773)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaagtgcca cctgggtcga cattgattat tgactagtaa cccgtgtcgg ctccagatct
       61 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
      121 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      181 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      241 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      301 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      361 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      421 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      481 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      541 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      601 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      661 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      721 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      781 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      841 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      901 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      961 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     1021 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1081 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1141 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1201 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1261 tttttgttag acgaagcttg ggctgcaggt cgagctcaag cttcgaattc atggcagtcg
     1321 gaatttatat tcgagttagt acccaagaac aggcgtccga aggccactct atagaatccc
     1381 aaaaaaagaa gctggccagc tactgcgaga ttcagggatg ggacgattat cgcttctaca
     1441 tcgaggaagg tatctccggg aaaaacacaa atcggccaaa gttgaaactg ctcatggaac
     1501 atattgaaaa gggaaagatt aatattctcc tcgtatatcg cctcgaccga ctcacacgct
     1561 ctgtgatcga tttgcacaag ctgcttaact ttttgcagga gcatgggtgt gcatttaaat
     1621 cagcgacaga gacctatgat acgacgacag cgaatggtcg catgtccatg ggtattgtca
     1681 gtctcttggc ccaatgggaa acagagaata tgtccgaacg gataaaactt aaccttgaac
     1741 acaaggtgct cgtcgaaggg gagagagtcg gagcgatccc ctacggattc gacctctccg
     1801 acgacgaaaa gcttgttaag aacgaaaaat ctgccatatt gctggacatg gtggaaagag
     1861 tcgagaacgg gtggtcagtc aatcggattg tcaactattt gaatcttact aataatgatc
     1921 ggaactggtc cccgaacggt gttctgcgcc tgctgcggaa cccggccctg tacggcgcca
     1981 cacggtggaa cgacaagatc gctgaaaaca cgcacgaagg cattatctct aaagaaagat
     2041 ttaatcggct tcagcagata ttggcagacc gctccataca ccaccgaagg gacgtgaaag
     2101 gtacttatat atttcaaggg gtcttgcgat gtcccgtctg cgaccaaacc ctgagtgtaa
     2161 accgctttat taaaaaacgg aaggatggga cggagtattg cggcgtcctg tatagatgcc
     2221 aaccatgcat aaaacagaat aaatacaatc ttgccatcgg agaagctcga ttcctgaagg
     2281 ctctcaatga atatatgtcc acagtagagt ttcagacggt tgaggatgaa gtaataccca
     2341 aaaaaagtga gcgagaaatg ctcgagtcac agcttcaaca gatcgctaga aagcgagaga
     2401 agtaccaaaa ggcgtgggcc tcagacctta tgagtgacga tgagtttgaa aagctgatgg
     2461 tagaaacgag agaaacgtat gatgagtgta aacaaaagct tgagtcttgt gaagacccaa
     2521 taaagatcga cgagacttac cttaaagaga tagtttatat gttccaccag acgttcaacg
     2581 atctggagtc tgagaagcaa aaagaattta tctcaaagtt tattcgcacg attcgataca
     2641 ctgtaaagga gcaacaacca attcggccgg ataaatctaa gacggggaaa gggaagcaga
     2701 aagttatcat aacggaggtt gagttctatc agtaagcggc cgcactcctc aggtgcaggc
     2761 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     2821 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     2881 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     2941 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     3001 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     3061 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     3121 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     3181 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     3241 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     3301 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3361 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3421 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     3481 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3541 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3601 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3661 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3721 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3781 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     3841 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3901 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3961 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4621 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4681 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4741 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4801 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4861 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4921 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4981 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5041 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5101 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5161 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5221 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5281 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5341 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5401 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5461 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5521 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5581 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5641 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5701 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5761 taggggttcc gcgcacattt ccccg
//
