LOCUS       sequence-250932-         163 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit 
            including promoter Pbad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 163)
  AUTHORS   Liu Y, Wan X, Wang B
  TITLE     Engineered CRISPRa enables programmable eukaryote-like gene 
            activation in bacteria.
  JOURNAL   Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0.
  PUBMED    31451697
REFERENCE   2  (bases 1 to 163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-26"; volume: "10(1):3693. doi"; pages: " 
            10.1038/s41467-019-11479-0"
COMMENT     Sequence Label: pLY124
FEATURES             Location/Qualifiers
     source          1..163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        38..56
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        108..126
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 aaagctgttg tggcaaaaaa gtttgagagc taggccaaca tgaggatcac ccatgtctgc
       61 agggcctagc aagttcaaat aaggctagtc cgttatcaac ttggccaaca tgaggatcac
      121 ccatgtctgc agggccaagt ggcaccgagt cggtgctttt ttt
//
