LOCUS       sequence-250970-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Engineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including 
            promoter Plux2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Liu Y, Wan X, Wang B
  TITLE     Engineered CRISPRa enables programmable eukaryote-like gene 
            activation in bacteria.
  JOURNAL   Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0.
  PUBMED    31451697
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-26"; volume: "10(1):3693. doi"; pages: " 
            10.1038/s41467-019-11479-0"
COMMENT     Sequence Label: pLY77
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             853..864
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(876..910)
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     terminator      918..945
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ggaccaaaac gaaaaaagac gcttttcagc gtcttattgt tcgtctttgg taccgagctc
       61 tagtagtgat ctacactagc actatcagtg ttattaattt ttaaagtatg ggcaatcaat
      121 tgctcctgtt aaaattgctt tagaaatact ttggcagcgg tttgttgtat tgagtttcat
      181 ttgcgcattg gttaaatgga aagtgacagt acgctcactg caacctaata tttttgaaat
      241 atcccaagag ctttttcctt cgcatgccca cgctaaacat tctttttctc ttttggttaa
      301 atcgttgttt gatttattat ttgctatatt tatttttcga taattatcaa ctagagaagg
      361 aacaattaat ggtatgttca tacacgcatg taaaaataaa ctatctatat agttgtcttt
      421 ttctgaatgt gcaaaactaa gcattccgaa gccattgtta gccgtatgaa tagggaaact
      481 aaacccagtg ataagacctg atgttttcgc ttctttaatt acatttggag attttttatt
      541 tacagcattg ttttcaaata tattccaatt aattggtgaa tgattggagt tagaataatc
      601 tactatagga tcatatttta ttaaattagc gtcatcataa tattgcctcc attttttagg
      661 gtaattatct aggattgaaa tatcagattt aaccatagaa tgaggataaa tgatcgcgag
      721 taaataatat tcacaatgta ccattttagt catatcagat aagcattgat taatatcatt
      781 attgcttcta caagctttaa ttttattaat tattctgtat gtgtcgtcgg catttatgtt
      841 tttcatctag tatttctcct ctttaatctc tagtagctag cacaatccct aggactgagc
      901 tagctgtcaa tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga
      961 cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt tactttcgaa ta
//
