LOCUS       sequence-251081-        7650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Hughes CM, Rozenblatt-Rosen O, Milne TA, Copeland TD, Levine SS, Lee
            JC, Hayes DN, Shanmugam KS, Bhattacharjee A, Biondi CA, Kay GF, 
            Hayward NK, Hess JL, Meyerson M
  TITLE     Menin associates with a trithorax family histone methyltransferase 
            complex and with the hoxc8 locus.
  JOURNAL   Mol Cell. 2004 Feb 27. 13(4):587-97.
  PUBMED    14992727
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Feb 27. 13(4):587-97."
COMMENT     Sequence Label: pBABE hygro MEN1 A309P
FEATURES             Location/Qualifiers
     source          1..7650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             93..562
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(677..1468,1469..1537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1300..1319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1538..1642)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1673..2142
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2207..2406
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2607..3023
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2953..2975
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2991..3007
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      3182..3191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5512..5532)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5517..5846
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5697..5832
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5920..6945
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5920..6945
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     7462..7481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
       61 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
      121 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
      181 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
      241 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
      301 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
      361 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
      421 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
      481 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
      541 acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct
      601 taagtattta cattaaatgg ccatagtgcg gccgcaaatc aatctaaagt atatatgagt
      661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
      721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
      781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
      841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
      901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
      961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     1021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     1081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     1141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     1201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     1261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     1321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     1381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     1441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     1501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     1561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     1621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgca gcctgaatat
     1681 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     1741 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     1801 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     1861 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     1921 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     1981 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     2041 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     2101 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
     2161 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggcaagc tggccagcaa
     2221 cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt
     2281 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
     2341 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
     2401 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
     2461 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
     2521 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
     2581 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     2641 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     2701 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     2761 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     2821 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     2881 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     2941 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     3001 ccctcactcc ttctctaggc gccggccgga tcccagtgtg gtggtacgta ggaattcccg
     3061 ggtcgaccca cgcgtccggt gtccggagcc gcggacctag agatcccaga agccacagcg
     3121 cagcggcccg gcccgccact atttccaggc tctgcggggc aggggccgcc gcccaccgcc
     3181 cgccgccatg gggctgaagg ccgcccagaa gacgctgttc ccgctgcgct ccatcgacga
     3241 cgtggtgcgc ctgtttgctg ccgagctggg ccgagaggag ccggacctgg tgctcctttc
     3301 cttggtgctg ggcttcgtgg agcattttct ggctgtcaac cgcgtcatcc ctaccaacgt
     3361 tcccgagctc accttccagc ccagccccgc ccccgacccg cctggcggcc tcacctactt
     3421 tcccgtggcc gacctgtcta tcatcgccgc cctctatgcc cgcttcaccg cccagatccg
     3481 aggcgccgtc gacctgtccc tctatcctcg agaagggggt gtctccagcc gtgagctggt
     3541 gaagaaggtc tccgatgtca tatggaacag cctcagccgc tcctacttca aggatcgggc
     3601 ccacatccag tccctcttca gcttcatcac aggcaccaaa ttggacagct ccggtgtggc
     3661 ctttgctgtg gttggggcct gccaggccct gggtctccgg gatgtccacc tcgccctgtc
     3721 tgaggatcat gcctgggtag tgtttgggcc caatggggag cagacagctg aggtcacctg
     3781 gcacggcaag ggcaacgagg accgcagggg ccagacagtc aatgccggtg tggctgagcg
     3841 gagctggctg tacctgaaag gatcatacat gcgctgtgac cgcaagatgg aggtggcgtt
     3901 catggtgtgt gccatcaacc cttccattga cctgcacacc gactcgctgg agcttctgca
     3961 gctgcagcag aagctgctct ggctgctcta tgacctggga catctggaaa ggtaccccat
     4021 ggccttaggg aacctggcag atctagagga gctggagccc acccctggcc ggccagaccc
     4081 actcaccctc taccacaagg gcattgcctc acccaagacc tactatcggg atgaacacat
     4141 ctacccctac atgtacctgg ctggctacca ctgtcgcaac cgcaatgtgc gggaagccct
     4201 gcaggcctgg gcggacacgg ccactgtcat ccaggactac aactactgcc gggaagacga
     4261 ggagatctac aaggagttct ttgaagtagc caatgatgtc atccccaacc tgctgaagga
     4321 ggcagccagc ttgctggagg cgggcgagga gcggccgggg gagcaaagcc agggcaccca
     4381 gagccaaggt tccgccctcc aggaccctga gtgcttcgcc cacctgctgc gattctacga
     4441 cggcatctgc aaatgggagg agggcagtcc cacgcctgtg ctgcacgtgg gctgggccac
     4501 ctttcttgtg cagtccctag gccgttttga gggacaggtg cggcagaagg tgcgcatagt
     4561 gagccgagag gccgaggcgg ccgaggccga ggagccgtgg ggcgaggaag cccgggaagg
     4621 ccggcggcgg ggcccacggc gggagtccaa gccagaggag cccccgccgc ccaagaagcc
     4681 agcactggac aagggcctgg gcaccggcca gggtgcagtg tcaggacccc cccggaagcc
     4741 tcctgggact gtcgctggca cagcccgagg ccctgaaggt ggcagcacgg ctcaggtgcc
     4801 agcacccgca gcatcaccac cgccggaggg tccagtgctc actttccaga gtgagaagat
     4861 gaagggcatg aaggagctgc tggtggccac caagatcaac tcgagcgcca tcaagctgca
     4921 actcacggca cagtcgcaag tgcagatgaa gaagcagaaa gtgtccaccc ctagtgacta
     4981 cactctgtct ttcctcaagc ggcagcgcaa aggcctctga actactgggg acttcggacc
     5041 gcttgtgggg acccaggctc cgcccttagt cccccaactc tgagcccatg ttctgccccc
     5101 agcccaaagg ggacaggcct cacctctacc caaaccctag gttcccggtc ccgagtacag
     5161 tctgtatcaa acccacgatt ttctccagct cagaacccag ggctctgccc cagtcgttag
     5221 aatataggtc tcttctccca gaatcccagc cggccaatgg aaacctcacg ctgggtccta
     5281 attaccagtc tttaaaggcc cagcccctag aaacccaagc tcctcctcgg aaccgctcac
     5341 ctagagccag accaacgtta ctcagggctc ctcccagctt gtaggagctg aggtttcacc
     5401 cttaacccaa ggagcacagg tcccacctcc agcccgggag cctaggacca ctcagcccct
     5461 aggagtatat ttccgcactt cagaattcgc cagcacagtg gtcgaccctg tggaatgtgt
     5521 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5581 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5641 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5701 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5761 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5821 tttttggagg cctaggcttt tgcaaaaagc ttgccagctg gggcgccctc tggtaaggtt
     5881 gggaagccct gcagatctga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
     5941 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     6001 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     6061 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     6121 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaatttag cgagagcctg
     6181 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     6241 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     6301 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     6361 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     6421 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     6481 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     6541 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     6601 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     6661 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     6721 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     6781 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     6841 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     6901 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg
     6961 ggatctatcg atgcggcggg tgtggtggtt acgcgtaatc tgctgcttgc aaacaaaaaa
     7021 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7081 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7141 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7201 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7261 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7321 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7381 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7441 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7501 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7561 aaacgccagc aacgcatcga taaaataaaa gattttattt agtctccaga aaaagggggg
     7621 aatgaaagac cccacctgta ggtttggcaa
//
