LOCUS       sequence-25114-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechnol J. 2008 Oct;3(9-10):1280-5. doi: 10.1002/biot.200800140.
  PUBMED    18729046
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2008-10"; volume: "3(9-10):1280-5. doi"; pages: " 
            10.1002/biot.200800140"
COMMENT     Sequence Label: pFA6a-bleMX6-P81nmt1-3FLAG
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(89..286)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(292..666)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVSDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVDEEQD"
ORIGIN
        1 gaacgggcgc atggcactgt ggatctgata tcatcgatga attcgagctc gtttaaactg
       61 gatggcggcg ttagtatcga atcgacagca gtatagcgac cagcattcac atacgattga
      121 cgcatgatat tactttctgc gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa
      181 aaaaaatata aatcacgcta acatttgatt aaaatagaac aactacaata taaaaaaact
      241 atacaaatga caagttcttg aaaacaagaa tctttttatt gtcagtactg atcagtcctg
      301 ctcctcgtcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga actcccgccc
      361 ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga agttcgtgga
      421 cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca cccaggccag
      481 ggtgttgtcc gacaccacct ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg
      541 gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc ggtcggtcca
      601 gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt
      661 ggccatggtt gtttatgttc ggatgtgatg tgagaactgt atcctagcaa gattttaaaa
      721 ggaagtatat gaaagaagac ctgngggcaa tcctantant ttttttctct acaggggcgc
      781 ggcgtgggga cattcaacgc gtctgtgagg gagcgtttcc c
//
