LOCUS       sequence-251366-        4187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - regulatory plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pSPB849
FEATURES             Location/Qualifiers
     source          1..4187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             50..61
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    108..129
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             join(321..323,324..326,327..1037)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(366..387)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(627..646)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     974..995
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1038..1094
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    2059..2079
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(join(2307..3098,3099..3167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2930..2949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3168..3272)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(3316..3347)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(3491..3508)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3625..4170)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3705..3724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acggaaaaaa aaaccccgcc cctgacaggg cggggttttt tttccaatta ttgaaggccg
       61 ctaacgcggc ctttttttgt ttctggtctg ccttaatcaa tgactaagaa ttcgcggccg
      121 cttctagagc gtctcacttc ggatccgctg caggcttccg cacccatagc atttttatcc
      181 ataagattag cggatcctac cttttacttc ttgccatcga ctacggatac tgagcacgac
      241 atgcatgcca cttaaaaagg agatcaacaa tgggtggggg cggctcaggc ggcggtggtt
      301 ccggtggtgg cggctcttca atggtgagca agggcgagga gctgttcacc ggggtggtgc
      361 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      421 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      481 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      541 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      601 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      661 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      721 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      781 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      841 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      901 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      961 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1021 tggacgagct gtacaagaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
     1081 cgggcccggg atccatgtcc gaagggcgtc gtggtctggg tcgagggctt tcggccctgc
     1141 tgggcgaggt cgacgccgcg ccggctcagg cgccgggcga gcagcttggc ggttcccgtg
     1201 aagcgccgat cgaaatcctg cagcgcaatc ccgaccagcc ccgtcggacc ttccgcgagg
     1261 aagacctcga ggatctgtca aactcgatcc gtgagaaggg cgttctgcag ccgatcctgg
     1321 tgcggccgtc gccggatacg gctggcgagt accagatcgt cgccggcgag cgccgttggc
     1381 gcgcagccca gcgggcgggc ctcaagactg tgccgatcat ggtgcgcgag ctcgacgacc
     1441 tggcggtgct ggagatcggc atcatcgaga acgtccagcg ggctgatctg aacgtcctgg
     1501 aagaagcgct ctcctacaag gtgctgatgg agaagttcga gcgcacccag gagaacatcg
     1561 cccagaccat cggcaagagc cgcagccacg tggccaacac gatgcgcttg ctggccctac
     1621 cggacgaggt tcagtcgcac ctggtcagtg gtgagctgac ggccggccac gcccgcgcca
     1681 tcgctgcagc ggctgacccg gtggccctcg ccaagcaaat catcgagggc ggcttgtcgg
     1741 tgcgcgagac agaggccctg gcccgtaagg cgcccaacct gtctgcgggc aagagcaagg
     1801 ggggccgccc cccacgcgtg aaggacaccg acacccaggc gctggagtct gacctgtcct
     1861 cggttctggg tctggatgtc tcgatcgacc atcgcgggtc taccggcacg ctgaccatca
     1921 cctacgccac cctcgagcag ctcgacgacc tctgcaaccg actgacgcgc gggatctaat
     1981 aatggcctcg gtaccaaaga cgaacaataa gacgctgaaa agcgtctttt ttcgttttgg
     2041 tccgctgcgt ttgagacgta ctagtagcgg ccgctgcagt taatcactga ttaactcggt
     2101 accaaattcc agaaaagagg cctcccgaaa ggggggcctt ttttcgtttt ggtccttttg
     2161 ttatcaataa aaaaggggag cggtttcccg ctccccttat tgttcgtcta cagattatca
     2221 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2281 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2341 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2401 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggga cccacgctca
     2461 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2521 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2581 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2641 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2701 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2761 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2821 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2881 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2941 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3001 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3061 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3121 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgga
     3301 gtcctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tgcttaccca
     3361 atagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt
     3421 catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat
     3481 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
     3541 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
     3601 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     3661 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3721 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     3781 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
     3841 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     3901 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
     3961 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
     4021 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
     4081 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
     4141 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaa
//
