LOCUS       sequence-251418-       16087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16087)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX083
FEATURES             Location/Qualifiers
     source          1..16087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6201..6225)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6254..6343
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6377..6629
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7058..7310)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7778..8803)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7778..8803)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8813..9157)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9299..9316)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9299..9315)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9308..9330)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9455..9474)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9665..9686
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9933..9957
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11553..12701)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13000..13794)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13986..14753
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15446..16077
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggagaa
     4621 cagtaagagc actgcagggt tttcttctcc aagaagtgat aatttcccgg ctggtttacg
     4681 ggttcttgtt gttgatgatg atcctacctg gttgaaaatt cttgaaaaga tgcttaagaa
     4741 gtgctcttat gaagtgacga catgtggtct agcacgagag gctctgaatc tgctccgaga
     4801 aagaaaggat gggtttgaca ttgtgattag tgatgttaac atgcctgaca tggatggatt
     4861 taagcttctt gagcatgttg gacttgagat ggatcttcct gtcataatga tgtctgttga
     4921 tggagaaaca agcagggtaa tgaagggcgt tcaacacggt gcatgtgatt atctcttaaa
     4981 gcctatacgg atgaaagaac ttagaaatat atggcagcat gtcctcagaa aaaggatgca
     5041 cgaggcaagg gacatcggaa accatgaaat ggaccaattt gatgaagtct ggatgcttaa
     5101 tggaactgaa ctcctttccg gaaagaagag aaaagatttt gacaatgaaa aagaaattag
     5161 tgactcaaga tgtgttgatt cttccatgaa gaaagccaga gtagtttgga ctgtagatct
     5221 tcatcagaaa tttgtcaaag ccgtaaacca gattggattt gacaaagctg gtcccaagaa
     5281 gatactggac ttgatgggtg ttccatggtt gactagagaa aatgttgcta gccacttaca
     5341 gaagtatcgc ctatacttga ctaggttgca gaaagaaaat gaagtcaaag cttcattttg
     5401 tgggatgaag catccgaatg tttcttctaa acaaaattct cctcagaatc cagtggaggt
     5461 gtgcactgat gttacaaatg acaagtgtag tggtgtttct ggagacaagg ctattgttca
     5521 aaatgggaag gccaacatat ataagagcaa cgtaaagggt gttgtttcaa tgccagtaac
     5581 agcgtctagg tctgtggttg gagataactt tggttcaaag aaaagcctca gtgattcctt
     5641 tggatatttc aaagcacgtt ttccgtcaga aattgggcta tgtaatcagc cactgcctgg
     5701 gatggattgc acacaaccaa cttgttctct caactttgta ccttctcatg aagtgaggga
     5761 cagagtttcc agtaaagaaa ataagccttc tttcttcaag agtagaagtg gagtaggaaa
     5821 attatctgca ttggaagcca ttcaacctgt gagccatcaa ataaattttc atgctcttga
     5881 gcaaattcca agtactacat ggagtatgac gagtcaaaat gtagatcaaa atctagtcaa
     5941 tggtctgcaa tcaatcccag gaaatctaac tttgggaagt ggatcagttg ttgcatctct
     6001 aggtgaggat gattctattg aatgttttcc agcttataat gattgtcttc ggaacataca
     6061 acaatttgac tacagtgatc ctcaaaccat ttctggagtt ccaacatgct tgtatgatac
     6121 attgaggttt gattatgagt atccaaatga ttcattggaa ggtattgtgt tggaccaagg
     6181 actgttcata gtccccgggg acccagcttt cttgtacaaa gtggttgata acagcgggtt
     6241 aattaacatc ttttacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     6301 ggactatgca ggatcctatc catatgacgt tccagattac gctgctcagt gcagcgctta
     6361 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     6421 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     6481 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     6541 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     6601 gcgcgcggtg tcatctatgt tactagatcg ggaattagct tcatcaacgc aagacatgcg
     6661 cacgaccgtc tgacaggaga ggaatttccg acgagcacag aaaggacttg ctcttggacg
     6721 taggcctatt tctcaggcac atgtatcaag tgttcggacg tgggttttcg atggtgtatc
     6781 agccgccgcc aactgggaga tgaggaggct ttcttggggg gcagtcagca gttcatttca
     6841 caagacagag gaacttgtaa ggagatgcac tgatttatct tggcgcaaac cagcaggacg
     6901 aattagtggg aatagcccgc gaatatctaa gttatgcctg tcggcatgag cagaaacttc
     6961 caattcgaaa cagtttggag aggttgtttt tgggcatacc ttttgttagt cagcctctcg
     7021 attgctcatc gtcattacac agtaccgaag tttgatcgat ctagtaacat agatgacacc
     7081 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     7141 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     7201 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     7261 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttcgacg
     7321 cactccttct ttactccacc atctcgtcct tattgaaaac gtgggtagca ccaaaacgaa
     7381 tcaagtcgct ggaactgaag ttaccaatca cgctggatga tttgccagtt ggattaatct
     7441 tgcctttccc cgcatgaata atattgatga atgcatgcgt gaggggtatt tcgattttgg
     7501 caatagctgc aattgccgcg acatcctcca acgagcataa ttcttcagaa aaatagcgat
     7561 gttccatgtt gtcagggcat gcatgatgca cgttatgagg tgacggtgct aggcagtatt
     7621 ccctcaaagt ttcatagtca gtatcatatt catcattgca ttcctgcaag agagaattga
     7681 gacgcaatcc acacgctgcg gcaaccttcc ggcgttcgtg gtctatttgc tcttggacgt
     7741 tgcaaacgta agtgttggat cccggtcggc atctactcta ttcctttgcc ctcggacgag
     7801 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     7861 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     7921 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     7981 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
     8041 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     8101 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     8161 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     8221 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     8281 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     8341 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     8401 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
     8461 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     8521 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
     8581 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     8641 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     8701 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
     8761 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatcgggg tcgtcctctc
     8821 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     8881 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     8941 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     9001 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     9061 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     9121 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     9181 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tgacggatct
     9241 ctaggacgcg tcgacgcgtc gacgcgtcga cgcgtcgacg cgtcctagaa gctaattcac
     9301 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     9361 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     9421 cttcccaaca gttgcgcagc ctgaatggcg cccgctcctt tcgctttctt cccttccttt
     9481 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9541 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     9601 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9661 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     9721 gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt cgcctgctgg
     9781 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     9841 agctgttgcc cgtctcactg gtgaaaagaa aaaccacccc agtacattaa aaacgtccgc
     9901 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     9961 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
    10021 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
    10081 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
    10141 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat
    10201 catctccctc gcagagatcc gaattatcag ccttcttatt catttctcgc ttaaccgtga
    10261 caggctgtcg atcttgagaa ctatgccgac ataataggaa atcgctggat aaagccgctg
    10321 aggaagctga gtggcgctat ttctttagaa gtgaacgttg acgatatcaa ctcccctatc
    10381 cattgctcac cgaatggtac aggtcgggga cccgaagttc cgactgtcgg cctgatgcat
    10441 ccccggctga tcgaccccag atctggggct gagaaagccc agtaaggaaa caactgtagg
    10501 ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc
    10561 gagccacgcc aggccgagaa cattggttcc tgtaggcatc gggattggcg gatcaaacac
    10621 taaagctact ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag
    10681 cagaggcacg ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc
    10741 ccccgccagg cccgctgcga cgccgacagg atctagcgct gcgtttggtg tcaacaccaa
    10801 cagcgccacg cccgcagttc cgcaaatagc ccccaggacc gccatcaatc gtatcgggct
    10861 acctagcaga gcggcagaga tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc
    10921 cgcgaccccg cccggcaggc ggtagaccga aataaacaac aagctccaga atagcgaaat
    10981 attaagtgcg ccgaggatga agatgcgcat ccaccagatt cccgttggaa tctgtcggac
    11041 gatcatcacg agcaataaac ccgccggcaa cgcccgcagc agcataccgg cgacccctcg
    11101 gcctcgctgt tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg
    11161 gccgtcctcc tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa
    11221 tgccacggca tctcgcaacc gttcagcgaa cgcctccatg ggctttttct cctcgtgctc
    11281 gtaaacggac ccgaacatct ctggagcttt cttcagggcc gacaatcgga tctcgcggaa
    11341 atcctgcacg tcggccgctc caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt
    11401 taatcctctg tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg
    11461 aagcaagtgc gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct
    11521 gaacccccag ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca
    11581 ttgacccagg cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc
    11641 tgctcgcgcc acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc
    11701 agcttgagcg ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc
    11761 ggcgacagct tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc
    11821 acgacgattt cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg
    11881 acgcggaagc ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag
    11941 cccatcgccg tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca
    12001 ttgatcgacc agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg
    12061 ataggggtgc gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc
    12121 cgcagctcga cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg
    12181 ttttgcagcg cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc
    12241 gtgtcgtttg gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc
    12301 tgcatttcct tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg
    12361 acctgttttg ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc
    12421 gtgtcgatgg tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc
    12481 tccacggcgg ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg
    12541 atcttggccg tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca
    12601 cgcatgacgg tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc
    12661 agttcttgcc tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt
    12721 gccccgactc acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg
    12781 gtgtccagat aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc
    12841 ttctcgtact tggtattccg aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa
    12901 tacttgccgt gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc
    12961 tcctgcttgt cgccggcatc gttgcgccac atctaggtac taaaacaatt catccagtaa
    13021 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
    13081 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
    13141 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
    13201 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
    13261 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
    13321 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
    13381 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
    13441 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
    13501 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
    13561 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
    13621 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
    13681 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
    13741 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
    13801 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
    13861 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
    13921 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccacaa
    13981 ttatgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
    14041 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
    14101 ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa aacatggcaa
    14161 ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc
    14221 atgaatggcg ttggatgccg ggcaacagcc cgcattatgg gcgttggcct caacacgatt
    14281 ttacgtcact taaaaaactc aggccgcagt cggtaacctc gcgcatacag ccgggcagtg
    14341 acgtcatcgt ctgcgcggaa atggacgaac agtggggcta tgtcggggct aaatcgcgcc
    14401 agcgctggct gttttacgcg tatgacagtc tccggaagac ggttgttgcg cacgtattcg
    14461 gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg
    14521 tgatatggat gacggatggc tggccgctgt atgaatcccg cctgaaggga aagctgcacg
    14581 taatcagcaa gcgatatacg cagcgaattg agcggcataa cctgaatctg aggcagcacc
    14641 tggcacggct gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag
    14701 tcatcgggca ttatctgaac ataaaacact atcaataagt tggagtcatt acccaattat
    14761 gatagaattt acaagctata aggttattgt cctgggtttc aagcattagt ccatgcaagt
    14821 ttttatgctt tgcccattct atagatatat tgataagcgc gctgcctatg ccttgccccc
    14881 tgaaatcctt acatacggcg atatcttcta tataaaagat atattatctt atcagtattg
    14941 tcaatatatt caaggcaatc tgcctcctca tcctcttcat cctcttcgtc ttggtagctt
    15001 tttaaatatg gcgcttcata gagtaattct gtaaaggtcc aattctcgtt ttcatacctc
    15061 ggtataatct tacctatcac ctcaaatggt tcgctgggtt tatcgcaccc ccgaacacga
    15121 gcacggcacc cgcgaccact atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca
    15181 tgaagtccgt gaatgccccg acggccgaag tgaagggcag gccgccaccc aggccgccgc
    15241 cctcactgcc cggcacctgg tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc
    15301 ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac tgccccgatc ccggcaatgg
    15361 caaggactgc cagcgctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc
    15421 cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc
    15481 cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata
    15541 aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa
    15601 cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc
    15661 cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta
    15721 gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc
    15781 tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac
    15841 gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc
    15901 cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat
    15961 ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt
    16021 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga
    16081 aaggcgc
//
