LOCUS       sequence-251491-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
  TITLE     Engineering Strategy and Vector Library for the Rapid Generation of 
            Modular Light-Controlled Protein-Protein Interactions.
  JOURNAL   J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033.
  PUBMED    31150735
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2019 May 29. pii: S0022-2836(19)30317-1. doi: 
            10.1016/j.jmb.2019.05.033."
COMMENT     Sequence Label: pOVC1_X_AsPT1-LOV2-EcSsra
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..480
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
ORIGIN
        1 accatggcca ccggtgaatt ctcaggctct ggatcccccg ggctggcaac cacactggaa
       61 cggatcgaga aaaatttcgt gattactgat ccgagactgc ctgacaaccc aatcattttt
      121 gcgagcgatt ccttcctgca gctgacagaa tattctcggg aagagatcct ggggcgcaat
      181 tgccgttttc tgcagggacc cgagacagac cgtgccactg ttcggaaaat cagagatgct
      241 attgacaacc agactgaagt gaccgttcag ctgatcaatt ataccaagag cggcaagaag
      301 ttctggaacg tgttccacct gcagccgatg cgcgattata agggcgacgt ccagtacttc
      361 attggcgtgc agctggatgg caccgaacgt cttcatggcg ccgctgagcg tgaggcggtc
      421 tgcctgatca aaaagacagc ctttcagatt gctgaggcag cgaacgacga aaattacttt
      481 ggacccgggt aa
//
