LOCUS       sequence-251519-       14724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway cloning destination vector to express enhancer driven 
            LexAGAD in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14724)
  AUTHORS   Poe AR, Tang L, Wang B, Li Y, Sapar ML, Han C
  TITLE     Dendritic space-filling requires a neuronal type-specific 
            extracellular permissive signal in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Sep 19;114(38):E8062-E8071. doi: 
            10.1073/pnas.1707467114. Epub 2017 Sep 5.
  PUBMED    28874572
REFERENCE   2  (bases 1 to 14724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1707467114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-09-19- 19"; volume: "114"; issue: "38"; pages: 
            "E8062-E8071"
COMMENT     Sequence Label: pDEST-APIC-LexAGAD
FEATURES             Location/Qualifiers
     source          1..14724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..306
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     185..204
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(347..471)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             781..801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(811..1062,1063..1416)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     protein_bind    4295..4680
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(4690..4708)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5160..5231,5633..5906,5981..6635,6697..7012,7216..7347,
                     7418..8032)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8811..9365,9366..9396)
                     /label=P element 5' end
     rep_origin      complement(9631..10219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9711..9730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10390..11181,11182..11250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11013..11032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11251..11355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11423..11441
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11479..11501)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11601..11620
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    11829..11898
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(12442..12472,12473..12674)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    13072..13457
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    13491..13615
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(13640..13645,13646..13663,13664..13670)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             13724..14383
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(13790..13809)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
       61 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      121 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      181 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      241 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      301 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
      361 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      421 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tgatgatctg
      481 gtctagcgag cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc
      541 aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga
      601 gcaagctaaa caatcgtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
      661 aagtaaatca actgcaacta ctgaaatctg ccggtgagat tgtacaccct tcttaaagat
      721 ccccttcaaa gtctaaattc ttccttttcg tttagccaag actcgagaat caaaatgcca
      781 cccaagaaga agcgaaaagt agaagatcca atgaaggctc tcacggcccg acaacaggaa
      841 gtttttgatt tgatacggga tcatatatcc caaacgggta tgcctccgac ccgcgcagag
      901 atagcacagc gactgggctt tcgatcgcct aacgccgcgg aggagcactt gaaggcactg
      961 gcccgcaagg gtgtcattga aatcgtgtcc ggtgcgagcc gcggaatccg gctgttgcag
     1021 gaggaggaag agggcctgcc actggtggga cgcgtggccg ctggcgagcc gctgctggcc
     1081 cagcaacaca tagagggaca ctatcaggtg gacccctcct tgtttaagcc aaatgctgat
     1141 ttcctgttgc gggtgtcggg aatgtccatg aaggacatcg gtattatgga tggtgacctc
     1201 ctcgccgtcc ataagacaca ggatgtcagg aacggccagg tagtcgttgc caggatagac
     1261 gatgaggtca ctgtgaaacg tctcaagaag caaggcaata aggtcgagct gctgccggag
     1321 aatagcgagt tcaagccgat cgtggtggat ctgcgacagc agtcctttac tatcgagggc
     1381 ttggccgtgg gtgtgatccg caacggagat tggctgggat ccatcgacag tgcggcccac
     1441 cacgacaaca gcaccattcc actggacttc atgcctcgtg acgcactgca tggatttgac
     1501 tggagcgagg aggatgatat gagcgacgga ctcccattcc tgaagaccga ccccaataac
     1561 aatggtttct ttggcgacgg cagcctgctg tgtatcctgc gaagtattgg ttttaagccg
     1621 gaaaattaca cgaacagtaa tgtcaatcgt cttcccacaa tgatcaccga ccgttacacg
     1681 ctggccagtc gcagtactac aagccgcctg ctgcaaagct atcttaacaa cttccacccc
     1741 tactgcccca tagtgcatag tcctactctc atgatgcttt acaacaatca gattgagata
     1801 gcatcgaagg accagtggca gatcctcttc aactgcattc tcgccatcgg cgcttggtgt
     1861 attgaaggcg agagcacaga tatcgacgtg ttttactatc aaaacgccaa gtcgcacctc
     1921 acctcgaagg tcttcgaaag tggaagcatt atcctcgtga cagccctcca tctgctgagt
     1981 cgttataccc agtggcgtca gaaaactaat acatcctaca actttcattc gttcagcatt
     2041 cggatggcga ttagcctggg acttaatcgt gacctcccaa gtagcttcag cgattcgtcg
     2101 atcctggaac agcgccggag gatttggtgg agtgtgtaca gctgggagat ccagctgtcc
     2161 ctcttgtacg gacgcagcat tcagctgagt cagaacacga tttccttccc gtccagcgtg
     2221 gacgacgttc aacggacaac tacgggcccg accatctacc acggtatcat tgagacggcc
     2281 cgcttgcttc aagtgtttac gaagatttat gagctggata agacagtgac cgcggagaag
     2341 agtccgatct gcgccaagaa atgcctgatg atctgcaatg agatagagga ggtgagccgc
     2401 caggcaccca agttcctgca gatggacata agcactacgg cgctgactaa tctcctcaaa
     2461 gaacacccct ggctgagttt cacccgcttc gagctgaagt ggaaacagct gagtctgatt
     2521 atatacgttt tgcgggactt tttcacaaat tttacgcaga agaagtcgca gcttgagcaa
     2581 gatcagaacg accatcagtc ctacgaggta aagaggtgca gcataatgct ctccgacgca
     2641 gcccagcgca ccgtgatgtc ggtgtcgtcc tatatggaca accataatgt gaccccttat
     2701 ttcgcctgga attgctccta ctatttgttc aacgccgtgt tggtcccaat caagactctt
     2761 ctgagcaact ccaaatcgaa cgccgagaat aacgagacag cacagctctt gcagcaaatt
     2821 aacaccgtgt tgatgctgtt gaagaaactc gctacattca agattcagac ttgcgaaaag
     2881 tacatccagg tgttggaaga ggtgtgcgcc ccattccttc tttcgcaatg cgcaattccc
     2941 ctcccgcaca taagttacaa taattccaac ggctccgcta tcaagaatat cgtcggctcc
     3001 gctacaatcg cgcagtatcc cacgctgcct gaggaaaatg ttaacaacat atcggtgaag
     3061 tatgtaagtc caggcagcgt cggcccgagc cctgtgccgc tgaaaagcgg agcctccttc
     3121 agcgatctgg tcaagctgct cagcaaccgc ccaccatccc gcaactcccc tgtcactata
     3181 ccccgatcga ccccgtcgca tcgtagcgtt accccattcc tgggacaaca gcagcaactg
     3241 cagagtctgg tgccacttac gccctcggca ttgtttggcg gagccaactt caaccagagt
     3301 ggtaacattg cggacagctc cttgagcttc acgtttacca acagctccaa cggtccaaac
     3361 ttgatcacga ctcagacaaa ctcccaggca ttgtcccagc ccattgccag ctcgaatgtg
     3421 catgataact ttatgaataa tgaaattact gcctccaaga ttgacgacgg caacaacagc
     3481 aagccccttt ccccaggctg gactgaccag acggcctaca atgctttcgg tatcaccact
     3541 ggcatgttca acaccacaac gatggacgac gtatataact atctttttga tgatgaggat
     3601 actccaccga acccaaagaa ggagtaatct agaattaatt aactaagcca gtcggcaatc
     3661 ggacgccttc gaaacatgca acactaatgt ttaattcaga tttcagcaga gacaagctaa
     3721 aacaccgacg agttgtaatc atttctgtgc gccagcatat atttcttata tacaacgtaa
     3781 tacataatta tgtaattcta gcatctcccc aacactcaca tacaaacaaa caaaaaatac
     3841 aaacacacaa aacgtattta cccgcacgca tccttggcga ggttgagtat gaaacaaaaa
     3901 caaaacttaa tttagagcaa agtaattaca cgaataaatt taataaaaaa aaactataat
     3961 aaaaacgcca atcatgttat ttccaaaaaa aaaaaaaaaa aaaaaactag acgagatgtt
     4021 tgcgcttgtg tcgatgaatg taaagaattg caactacata gatggtaatt aatttcagct
     4081 gtaagaagta caagtttttc ataattgtga gaaaagtgca gtagtatcgc ttaaagttca
     4141 gttttaatca gggcatttag cagctcgaac ccaatgtatt tgtaagccca ataaacgtgt
     4201 aaatgcctag agcaaaatat cacaactgta gcttttcatc cgaaagataa taatgaatag
     4261 actgacctga cgtttttcaa aatgcgtaac tagtcacgta ataagtgtgc gttgaattta
     4321 ttcgcaaaaa cattgcatat tttcggcaaa gtaaaatttt gttgcatacc ttatcaaaaa
     4381 ataagtgctg catacttttt agagaaacca aataattttt tattgcatac ccgtttttaa
     4441 taaaatacat tgcataccct cttttaataa aaaatattgc atactttgac gaaacaaatt
     4501 ttcgttgcat acccaataaa agattattat attgcatacc cgtttttaat aaaatacatt
     4561 gcataccctc ttttaataaa aaatattgca tacgttgacg aaacaaattt tcgttgcata
     4621 cccaataaaa gattattata ttgcatacct tttcttgcca taccatttag ccgatcaatt
     4681 ctagtatgta tgtaagttaa taaaacccat ttttgcggaa agtagataaa aaaaacattt
     4741 ttttttttta ctgcactgga tatcattgaa cttatctgat cagttttaaa tttacttcga
     4801 tccaagggta tttgatgtac caggttcttt cgattacctc tcactcaaaa tgacattcca
     4861 ctcaaagtca gcgctgtttg cctccttctc tgtccacaga aatatcgccg tctctttcgc
     4921 cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc
     4981 ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca agcggtttac gccatcaatt
     5041 aaacacaaag tgctgtgcca aaactcctct cgcttcttat ttttgtttgt tttttgagtg
     5101 attggggtgg tgattggttt tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa
     5161 tgggccaaga ggatcaggag ctattaattc gcggaggcag caaacaccca tctgccgagc
     5221 atctgaacaa tgtgagtagt acatgtgcat acatcttaag ttcacttgat ctataggaac
     5281 tgcgattgca acatcaaatt gtctgcggcg tgagaactgc gacccacaaa aatcccaaac
     5341 cgcaattgca caaacaaata gtgacacgaa acagattatt ctggtagctg ttctcgctat
     5401 ataagacaat ttttgagatc atatcatgat caagacatct aaaggcattc attttcgact
     5461 atattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct agatgcacaa
     5521 aaaataaata aaagtataaa cctacttcgt aggatacttc ggggtacttt ttgttcgggg
     5581 ttagatgagc ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag
     5641 cggagcggct tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac
     5701 gctccggcca cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa
     5761 cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg
     5821 atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc
     5881 gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg
     5941 aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg
     6001 ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc
     6061 cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat
     6121 ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac
     6181 ctctttatcg gctccctaac ggccagggaa cacctgattt tccaagccat ggtgcggatg
     6241 ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt
     6301 tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc
     6361 ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc taaccgatcc gccgcttctg
     6421 atctgcgatg agcccacctc cggactggac tcctttaccg cccacagcgt cgtccaggtg
     6481 ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc
     6541 gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg
     6601 ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg
     6661 tatctagtat tacataacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa
     6721 ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga
     6781 gtcccgtgat cggatcgcca agatatgcga caattttgcc attagcaaag tagcccggga
     6841 tatggagcag ttgttggcca ccaaaaatct ggagaagcca ctggagcagc cggagaatgg
     6901 gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct
     6961 gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg
     7021 ttccagtgga aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt
     7081 agatagaatt gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat
     7141 agttttgcag aagataaata aatttcattt aactcgcgaa tattaatgag atgcgagtaa
     7201 cattttaatt tgcagatggt tgccatcttg attggcctca tctttttggg ccaacaactc
     7261 acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc tcttcctgac caacatgacc
     7321 tttcaaaacg tctttgccac gataaatgta agtcatgttt agaatacatt tgcatttcaa
     7381 taatttacta actttctaat gaatcgattc gatttaggtg ttcacctcag agctgccagt
     7441 ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac acatactttc tgggcaaaac
     7501 gattgccgaa ttgccgcttt ttctcacagt gccactggtc ttcacggcga ttgcctatcc
     7561 gatgatcgga ctgcgggccg gagtgctgca cttcttcaac tgcctggcgc tggtcactct
     7621 ggtggccaat gtgtcaacgt ccttcggata tctaatatcc tgcgccagct cctcgacctc
     7681 gatggcgctg tctgtgggtc cgccggttat cataccattc ctgctctttg gcggcttctt
     7741 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
     7801 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
     7861 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
     7921 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gccattctca tcgtgagctt
     7981 ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt agccgacata
     8041 tatccgaaat aactgcttgt tttttttttt taccattatt accatcgtgt ttactgttta
     8101 ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta
     8161 tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta
     8221 accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa
     8281 caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac
     8341 ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca
     8401 cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc
     8461 gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta
     8521 tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct
     8581 aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt
     8641 acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa
     8701 atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat
     8761 acatttaatt tagaaaatgc ttggatttca ctggaactag aattaattcg gctgctgctc
     8821 taaacgacgc atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt
     8881 aaacaatgaa caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta
     8941 ctattcaaac ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg
     9001 gttcaggctc tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg
     9061 tctgaccttt tgcaggtgca gccttccact gcgaatcatt aaagtgggta tcacaaattt
     9121 gggagttttc accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac
     9181 acttcggcac gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt
     9241 tcattttttt ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca
     9301 acctttcctc tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg
     9361 gctatcgacg ggaccacctt atgttatttc atcatgggcc agacccacgt agtccagcgg
     9421 cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt
     9481 ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag
     9541 cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg
     9601 caccgagccc gagttcaaga agaaggcgtt tttccatagg ctccgccccc ctgacgagca
     9661 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9721 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9781 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9841 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9901 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9961 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10021 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10081 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10141 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10201 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10261 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10321 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10381 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10441 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10501 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10561 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10621 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10681 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10741 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10801 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10861 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10921 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10981 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11041 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11101 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11161 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11221 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11281 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11341 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    11401 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    11461 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    11521 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    11581 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accattcgac
    11641 atgcccgccg tgaccgtcga gaacccgctg acgctgcccc gcgtatccgc acccgccgac
    11701 gccgtcgcac gtcccgtgct caccgtgacc accgcgccca gcggtttcga gggcgagggc
    11761 ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc acctcgaccc gttcatcatg
    11821 atggaccaga tgggtgaggt ggagtacgcg cccggggagc ccaagggcac gccctggcac
    11881 ccgcaccgcg gcttcgagac cgtgacctac atcgtcgata tgcggtgtga aataccgcac
    11941 cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt
    12001 gcggagagcg ggtgggagac agcgaaagag caactacgaa acgtggtgtg gtggaggtga
    12061 attatgaaga gggcgcgcga tttgaaaagt atgtatataa aaaatatatc ccggtgtttt
    12121 atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga
    12181 agacaaatcc aaagtctact tgtggggatg ttcgaagggg aaatacttgt attctatagg
    12241 tcatatcttg tttttattgg cacaaatata attacattag ctttttgagg gggcaataaa
    12301 cagtaaacac gatggtaata atggtaaaaa aaaaaaacaa gcagttattt cggatatatg
    12361 tcggctactc cttgcgtcgg gcccgaagtc ttagagccag atatgcgagc acccggaagc
    12421 tcacgatgag aatggccaga ccatgatgaa ataacataag gtggtcccgt cggcaagaga
    12481 catccactta acgtatgctt gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg
    12541 tattgagtct gagtgagaca gcgatatgat tgttgattaa cccttagcat gtccgtgggg
    12601 tttgaattaa ctcataatat taattagacg aaattatttt taaagtttta tttttaataa
    12661 tttgcgagta cgcaaagctt cctaggtcaa gaccgacctg tccggtgccc tgaatgaact
    12721 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
    12781 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
    12841 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
    12901 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
    12961 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
    13021 agagcatcag gggctcgcgc cagccgaact gttcgccaag cttggctgca tcacgtaata
    13081 agtgtgcgtt gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt
    13141 gcatacctta tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat
    13201 tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata
    13261 ctttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt
    13321 ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa
    13381 caaattttcg ttgcataccc aataaaagat tattatattg catacctttt cttgccatac
    13441 catttagccg atcaattgtg ctcggcaaca gcatgctgca gcagatcatc acaagtttgt
    13501 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
    13561 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
    13621 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
    13681 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
    13741 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
    13801 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
    13861 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
    13921 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
    13981 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
    14041 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
    14101 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
    14161 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
    14221 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
    14281 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
    14341 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt
    14401 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
    14461 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
    14521 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
    14581 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
    14641 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
    14701 ctgacgagaa caggggctgg tgaa
//
