LOCUS       sequence-251725-       12640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RcCHAD-mCherry expression in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M
  TITLE     Molecular characterization of CHAD domains as inorganic 
            polyphosphate-binding modules.
  JOURNAL   Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi: 
            10.26508/lsa.201900385. Print 2019 Jun.
  PUBMED    31133615
REFERENCE   2  (bases 1 to 12640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-05-27"; volume: "2(3). pii: 2/3/e201900385. 
            doi"; pages: " 10.26508/lsa.201900385. Print 2019 Jun"
COMMENT     Sequence Label: pH7m34GW_RcCHAD-mCherry
FEATURES             Location/Qualifiers
     source          1..12640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2004..2024)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2044..2060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2044..2060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2057..2079)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     regulatory      2100..2109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2195..2219
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3550..4179
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4608..5681
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5747..5941
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6177..6199)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6285..6425
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6340..6359
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6440..6457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6611..7199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6691..6710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7445..8236)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8757..8781
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8874..9126)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9324..10349)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10385..10564)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     10930..10952
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10944..10961
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10945..10961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10969..10989
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(10991..11698)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(11269..11288)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    11700..11724
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(12631..12640,1..15))
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtacaaa cttgcctgtt aatcagaaaa actcagatta atcgacaaat tcgatcgcac
       61 aaactagaaa ctaacaccag atctagatag aaatcacaaa tcgaagagta attattcgac
      121 aaaactcaaa ttatttgaac aaatcggatg atatctatga aaccctaatc gagaattaag
      181 atgatatcta acgatcaaac ccagaaaatc gtcttcgatc taagattaac agaatctaaa
      241 ccaaagaaca tatacgaaat tgggatcgaa cgaaaacaaa atcgaagatt ttgagagaat
      301 aaggaacaca gaaatttacc ttgatcacgg tagagagaat tgagagaaag tttttaagat
      361 tttgagaaat tgaaatctga attgtgaaga agaagagctc tttgggtatt gttttataga
      421 agaagaagaa gaaaagacga ggacgactag gtcacgagaa agctaaggcg gtgaagcaat
      481 agctaataat aaaatgacac gtgtattgag cgttgtttac acgcaaagtt gtttttggct
      541 aattgcctta tttttaggtt gaggaaaagt atttgtgctt tgagttgata aacacgactc
      601 gtgtgtgccg gctgcaacca ctttgacgcc gtttattact gactcgtcga caaccacaat
      661 ttctaacggt cgtcataaga tccagccgtt gagatttaac gatcgttacg atttatattt
      721 ttttagcatt atcgttttat tttttaaata tacggtggag ctgaaaattg gcaataattg
      781 aaccgtgggt cccactgcat tgaagcgtat ttcgtatttt ctagaattct tcgtgcttta
      841 tttcttttcc tttttgtttt tttttgccat ttatctaatg caagtgggct tataaaatca
      901 gtgaatttct tggaaaagta acttctttat cgtataacat attgtgaaat tatccatttc
      961 ttttaatttt ttagtgttat tggatatttt tgtatgatta ttgatttgca taggataatg
     1021 acttttgtat caagttggtg aacaagtctc gttaaaaaag gcaagtggtt tggtgactcg
     1081 atttattctt gttatttaat tcatatatca atggatctta tttggggcct ggtccatatt
     1141 taacactcgt gttcagtcca atgaccaata atattttttc attaataaca atgtaacaag
     1201 aatgatacac aaaacattct ttgaataagt tcgctatgaa gaagggaact tatccggtcc
     1261 tagatcatca gttcatacaa acctccatag agttcaacat cttaaacaag aatatcctga
     1321 tctgaagaat gtggaggctt tagtcccttg gatacttggg aggctgtgga agaacagaaa
     1381 cgagctggtg cttaaaggga gggaatttgg aaccaatgag gtattagtaa ggacacaaga
     1441 agatgcagat gagtggatta gaaggaaaga ggctcagaat gtaaggaaag caccaaccac
     1501 aacaccgtcg ggacagacga gaaacatggg aagctccacc acaatcttgg gttaagtgca
     1561 actttgatgg ggcatggcca acagaaggat taaaatgtgg cttagggtgg gtgcttcgcg
     1621 atcatacagg gaaggtgtta tggttaggtg caagagctgt ggtaaaagta agaagcgtgc
     1681 tggaagtaga agtggaggct cttagatggg ctgtgctgtc attatcccga ttcaattata
     1741 ggaagatcat ttttgaggtg gattctcagc aacttgtgtc tttggttaca tgaaagttat
     1801 gcttgtcaag tctcaatcca attatccaag acataaagta tctacttagc aagtttgagg
     1861 attttatgct tgtgcataca agccgagaag gaaatggagt ggcagataga atagctaagg
     1921 aatctctttc ttttgagaat tatgatctaa agttgtattc tattgtacca atttgggtta
     1981 aaagctctgt tgagctagac tagcaacttt tctatacaaa gttgatagct tggcgtaatc
     2041 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc gggcccgcgg atcccgcggc
     2101 catggcggcc gggagcatgc gacgtcgggc ccaagcttag cttgagcttg gatcagattg
     2161 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     2221 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     2281 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     2341 taaagtactt taaagtactt taaagtactt tgatccaacc cctccgctgc tatagtgcag
     2401 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     2461 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     2521 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     2581 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     2641 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     2701 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     2761 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     2821 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     2881 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     2941 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     3001 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     3061 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     3121 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     3181 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     3241 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     3301 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     3361 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     3421 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     3481 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     3541 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     3601 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     3661 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     3721 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     3781 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     3841 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     3901 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     3961 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     4021 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     4081 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     4141 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     4201 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     4261 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     4321 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     4381 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     4441 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     4501 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
     4561 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     4621 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     4681 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     4741 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     4801 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     4861 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     4921 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     4981 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     5041 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     5101 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     5161 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     5221 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     5281 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     5341 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     5401 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     5461 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     5521 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     5581 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     5641 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     5701 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt
     5761 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     5821 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     5881 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     5941 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     6001 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     6061 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     6121 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6181 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     6241 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     6301 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     6361 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     6421 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6481 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6781 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7021 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7201 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7261 ggattttggt catgcatgat atatctccca atttgtgtag ggcttattat gcacgcttaa
     7321 aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca
     7381 tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta
     7441 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
     7501 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
     7561 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
     7621 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
     7681 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
     7741 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
     7801 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
     7861 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
     7921 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
     7981 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
     8041 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
     8101 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
     8161 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
     8221 tcaccgcttc ccccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
     8281 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     8341 cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg
     8401 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     8461 acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt gcccgaattg
     8521 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
     8581 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
     8641 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
     8701 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc
     8761 aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg
     8821 tttttaatgt actgaattaa cgccgaattg aattatcagc ttgcatgccg gtcgatctag
     8881 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     8941 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     9001 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     9061 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     9121 acgatctgct tgactctagg gaattaattc ctgaatcact gcgaccggcc ctcccgcgac
     9181 ccagccgagc gagcttagcg aactgtggac gagaactgtg ccaccaagcg taaggccgtt
     9241 ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc tgaggcgttc tcgaaatcag
     9301 ctcttgttcg gtcggcatct actctattcc tttgccctcg gacgagtgct ggggcgtcgg
     9361 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
     9421 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
     9481 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
     9541 ccaatgcgga gcatatacgc ccggagcctt ggcgatcctg caagctccgg atgcctccgc
     9601 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
     9661 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
     9721 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
     9781 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
     9841 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
     9901 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
     9961 gtctggctaa gatcggccgc agcgatagca tccatagcct ccgcgaccgg ctgaagaaca
    10021 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg
    10081 caataggtca ggctctcgct gaactcccca atgtcaagca cttccggaat cgggagcgcg
    10141 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
    10201 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
    10261 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca
    10321 gacgtcgcgg tgagttcagg ctttttcata ggggggattc gagttgagag tgaatatgag
    10381 actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa
    10441 tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg
    10501 tatgtgctta gctcattaaa ctccagaaac ccgcggctca gtggctcctt caacgttgcg
    10561 gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt
    10621 gactccctta attctccgct catgatcgat aattcgagct ctcccatatg gtcgacctgc
    10681 aggtcactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
    10741 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataagaacc
    10801 ctaattccct tatctgggaa ctactcacac attattctgg agaaaaatag agagagatag
    10861 atttgtagag agagactggt gatttttgcg gactctagca tggccgcggc tagtcagtta
    10921 gatcgttttc ccagtcacga cgttgtaaaa cgacggccag tgaattatca actatgtata
    10981 ataaagttgc ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
    11041 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
    11101 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
    11161 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
    11221 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
    11281 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
    11341 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
    11401 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
    11461 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
    11521 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
    11581 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
    11641 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacag
    11701 ccactttgta caagaaagct gggtagtgac gtaactgtgg tgcacgtaat gcgtgcaatc
    11761 ccgcgcgaat tgcatccagg tctgccgggc gcattgcctg ctgggccagt aagtacccgc
    11821 gagcgaactg tacggcttct gcggcttgag gttgttgttg ggctaattcc tgtaataaac
    11881 gtcccgctac taccaagtcg ttatggcgtc caagttcctc ctgggttgct gccaaagctt
    11941 tcagatatgc acgggttgat tttgagcgat acaaaccatg gaagaactct aaagcatagc
    12001 gaccacgctt ggtggcgatg cgggtacggt gagcggatgc tgcgtcatcg tcctgcatgc
    12061 gagctgcacg cttcaataag cttttgtgaa gatgttgcat gatttgacga gagaaatgag
    12121 cggctgaccc gtctaataat ggggctgttt caagcatcca agctcccagc gttaacatca
    12181 aacgtgtgta acgtggcgat aacagagcct gtgcagctgc atgacgcttt gcctgggcaa
    12241 ttttttgtac taatgcatta agctccaaca agccgttggc ccccgcaggc gctccatcga
    12301 tacgtggcag tgtagatgcc agcagaacat cccaatcacg cgctgccccc agctccgttc
    12361 ccagccaaga aatatcatcc tgaagggcag gcgggcatgg cgcgactgcg tcaaataatt
    12421 ttaaagcaga acgaaggcga cggactccaa cacgcatttg gtgcagcgtt tcggggtcat
    12481 gcccttcaat aaccgcgacc tcattacgct gaatgtgttc taagcagttt cccaggatcg
    12541 caggaaacgc ttcactcaga gcggcgtcgg cgtcaagtgc taatgcttga gcgcggaatg
    12601 gggcatgccc tgtctcgcga acaagcatta agcctgcttt
//
