LOCUS       sequence-25183-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.58
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..172)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(251..346)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(383..402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(384..402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(407..423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(407..423)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(525..544)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(603..622)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     gap             803..837
                     /estimated_length=35
ORIGIN
        1 tctngagctc gnttctacag gtcactaata ccatctaagt agttggttca tagtgactgc
       61 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      121 ttgatattta tatcatttta cgtttctcgt tcaactttct tgtacaaagt gggataattt
      181 ttttaaactt ttgaatgatt tttaataaaa tggctgagta gtttgggttt tcaaccggaa
      241 ttcaaatttt caactttatt atacaaagtt ggcattataa aaaagcattg cttatcaatt
      301 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttggag ctccatggta
      361 gcgttaacgc ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
      421 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
      481 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
      541 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
      601 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
      661 tgggaagccc gatgcgccag anttgtttct gaaacatggc aaangtagcg ttgccaatga
      721 tgttacagat gagatggtca gactaaactg gctganggaa tttatgcctc ttccgaccat
      781 caagcatttt nncnnnnnnn ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcn
      841 a
//
