LOCUS       sequence-25188-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.52
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..172)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(430..525)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(562..581)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(563..581)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(586..602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(586..602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(705..724)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tctngagctn nnntctacag gtcactaata ccatctaagt agttggttca tagtgactgc
       61 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      121 ttgatattta tatcatttta cgtttctcgt tcaactttct tgtacaaagt gggatagtcg
      181 aaaagttttc actcatgtgc aatgtttctc ttgttttata tttttagcca ttgtttttat
      241 aatttcattg attgtcgtgc ttttgtcttc tcttgtgtga tatcaatcta tcttcttcac
      301 gtatagtttc atctgtttga ttgagttatt tttctttcac tgtaagtctg gtgtttcgta
      361 gatttagaaa aaagtagcgt aataaattat tttgaaatgg accaaacatt caaatccagt
      421 tcagataaac aactttatta tacaaagttg gcattataaa aaagcattgc ttatcaattt
      481 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttggagc tccatggtag
      541 cgttaacgcg gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
      601 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaaatat
      661 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
      721 ccatattcaa cgggaaacgt cgangccgcg attaaattcc aacatggatg ctg
//
