LOCUS       sequence-25206-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.30
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..85
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    298..421
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(492..509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(492..508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(501..523)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      572..658
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tnnttttnac tgatagtgnn ctgttcgttg caacaaattg ataagcaatg cttttttata
       61 atgccaactt tgtataataa agttggtgta attaattgag ttaatttctt gcaacaatct
      121 agttcaaaca tttaccggga acaaggaaaa acaggttgaa taaataataa atgacagtca
      181 aggttttagg gattttacaa caagattgtt agaataaata atgtgcgtaa aacgtgtaag
      241 tatacaagaa gtgtatactt aaaaaacaaa ttaaacattc aatagttgga gtttatccca
      301 ctttgtacaa gaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      361 agattttgca taaaaaacag actacataat actgtaaaac acaacatatg cagtcactat
      421 gaaccaacta cttagatggt attagtgacc tgtagaattc gagctctaga gctgcagggc
      481 ccgagcttaa gactggccgt cgttttacaa cgtcgtgact gggaaaacat ccatgctagc
      541 gttaacgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcggaaga
      601 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      661 gccgggagcg gatttgaacg ttgtgaagcn cnnnnngnnn nnnggnngnn gggcaggacg
      721 cccgccataa actgcc
//
