LOCUS       sequence-252074-        4522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic 
            ""NOC"" part with coding regions for the three lambda red proteins 
            (the first; gam; doesn't have RBS; but bet and exo genes have RBS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-lambdaRED_NOC
FEATURES             Location/Qualifiers
     source          1..4522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             83..499
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             505..1290
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             1287..1967
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     misc_feature    1992..2012
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2046..2140
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        2213..2304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2305..2373,2374..3165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3169..3208
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            3355..3463
                     /note="incP origin of transfer"
     rep_origin      3499..4087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3988..4007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4241..4258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4375..4396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4430..4516)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc taatggatat taatactgaa actgagatca agcaaaagca
      121 ttcactaacc ccctttcctg ttttcctaat cagcccggca tttcgcgggc gatattttca
      181 cagctatttc aggagttcag ccatgaacgc ttattacatt caggatcgtc ttgaggctca
      241 gagctgggcg cgtcactacc agcagctcgc ccgtgaagag aaagaggcag aactggcaga
      301 cgacatggaa aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg atcatttgca
      361 acgccacggg gccagcaaaa aatccattac ccgtgcgttt gatgacgatg ttgagtttca
      421 ggagcgcatg gcagaacaca tccggtacat ggttgaaacc attgctcacc accaggttga
      481 tattgattca gaggtataaa acgaatgagt actgcactcg caacgctggc tgggaagctg
      541 gctgaacgtg tcggcatgga ttctgtcgac ccacaggaac tgatcaccac tcttcgccag
      601 acggcattta aaggtgatgc cagtgatgcg cagttcattg cattactgat cgttgccaac
      661 cagtacggcc ttaatccgtg gacgaaagaa atttacgcct ttcctgataa gcagaatggc
      721 atcgttccgg tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa ccagcagttt
      781 gatggcatgg actttgagca ggacaatgaa tcctgtacat gccggattta ccgcaaggac
      841 cgtaatcatc cgatctgcgt taccgaatgg atggatgaat gccgccgcga accattcaaa
      901 actcgcgaag gcagagaaat cacggggccg tggcagtcgc atcccaaacg gatgttacgt
      961 cataaagcca tgattcagtg tgcccgtctg gccttcggat ttgctggtat ctatgacaag
     1021 gatgaagccg agcgcattgt cgaaaatact gcatacactg ctgaacgtca gccggaacgc
     1081 gacatcactc cggttaacga tgaaaccatg caggagatta acactctgct gatcgccctg
     1141 gataaaacat gggatgacga cttattgccg ctctgttccc agatatttcg ccgcgacatt
     1201 cgtgcatcgt cagaactgac acaggccgaa gcagtaaaag ctcttggatt cctgaaacag
     1261 aaagccgcag agcagaaggt ggcagcatga caccggacat tatcctccag cgtaccggga
     1321 tcgatgtgag agctgtcgaa cagggggatg atgcgtggca caaattacgg ctcggcgtca
     1381 tcaccgcttc agaagttcac aacgtgatag caaaaccccg ctccggaaag aagtggcctg
     1441 acatgaaaat gtcctacttc cacaccctgc ttgctgaggt ttgcaccggt gtggctccgg
     1501 aagttaacgc taaagcactg gcctggggaa aacagtacga gaacgacgcc agaaccctgt
     1561 ttgagttcac ttccggcgtg aatgttactg aatccccgat catctatcgc gacgaaagta
     1621 tgcgtaccgc ctgctctccc gatggtttat gcagtgacgg caacggcctt gaactgaaat
     1681 gcccgtttac ctcccgggat ttcatgaagt tccggctcgg tggtttcgag gccataaagt
     1741 cagcttacat ggcccaggtg cagtacagca tgtgggtgac gcgaaaaaat gcctggtact
     1801 ttgccaacta tgacccgcgt atgaagcgtg aaggcctgca ttatgtcgtg attgagcggg
     1861 atgaaaagta catggcgagt tttgacgaga tcgtgccgga gttcatcgaa aaaatggacg
     1921 aggcactggc tgaaattggt tttgtatttg gggagcaatg gcgataagct ttgagacctt
     1981 tcatcgcgac ctactagtag cggccgctgc agggagttgt cttcgaagac ttcgctctag
     2041 tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag
     2101 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc ccaataatta
     2161 cgatttaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta
     2221 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2281 caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc gctgattccg
     2341 ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt gaaagtgaaa
     2401 gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct gaacagcggc
     2461 aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac ctttaaagtg
     2521 ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact gggccgtcgt
     2581 attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa acatctgacc
     2641 gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga taacaccgcg
     2701 gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt tctgcataac
     2761 atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga agcgattccg
     2821 aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg taaactgctg
     2881 acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat ggaagcggat
     2941 aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat tgcggataaa
     3001 agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc ggatggtaaa
     3061 ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga tgaacgtaac
     3121 cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga tacaattaaa
     3181 ggctcctttt ggagcctttt tttttggacg acccttgtcc ttttccgctg cataaccctg
     3241 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     3301 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     3361 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     3421 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg gccccgtaga
     3481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3601 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4141 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4201 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4261 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     4321 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     4381 gagttagctc actcattagg caggcgcgcc cagctgtcta gggcggcgga tttgtcctac
     4441 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4501 cctttcgttt tatttgatgc ct
//
