LOCUS       sequence-252103-        5994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of mouse Ift52.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ye X, Zeng H, Ning G, Reiter JF, Liu A
  TITLE     C2cd3 is critical for centriolar distal appendage assembly and 
            ciliary vesicle docking in mammals.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2164-9. doi: 
            10.1073/pnas.1318737111. Epub 2014 Jan 27.
  PUBMED    24469809
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1318737111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-11- 11"; volume: "111"; issue: "6"; pages: "2164-9"
COMMENT     Sequence Label: pEGFPC1-Ift52
FEATURES             Location/Qualifiers
     source          1..5994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(294..315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(555..574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     902..923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2418..2539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2455..2474)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2509..2528
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2546..3001)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2683..2704)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2895..2914
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3028..3132
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3134..3491
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3134..3325
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3157..3177)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3342..3477
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3526..4320
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3526..4320
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3580..3599)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4190..4209
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4508..4527)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4552..4599
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4928..5516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5417..5436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5691..5994
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      421 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      841 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      961 acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac gagaaggagc
     1021 tgcggagtac cattcttttc aatgcataca aaaaggaggt gtttaccacc aacactggct
     1081 acaaatcttt gcagaaaaga cttcggagta attggaagat tcagagcttg aaagatgaaa
     1141 tcacttctga gaagttaatt ggggtgaaac tatggatcac agctggacca agggaaaagt
     1201 ttactgctgc tgaatttgaa gtcctgaaga agtatcttga cagtggtggg gatatccttg
     1261 tgatgctggg agaaggggga gaatccagat ttgataccaa tattaacttt cttctagagg
     1321 aatacggaat catggttaat aatgatgctg tggttagaaa cgtgtattac aagtacttcc
     1381 atcctaagga agctctggtt tcagacggag tgttgaacag agaaattagc cgagctgcag
     1441 gaaaggctgt gcctggagtc attgatgaag agaacagtgg gaacaatgcc caggctctca
     1501 cctttgtcta cccttttggt gccacattga gcgtcatgaa gccagcagtg gccgtgctat
     1561 ccacaggctc tgtctgcttc ccactgaaca gacccattct agctttctat cactcaaaga
     1621 accaaggttt tggaaagctg gcagtgcttg gttcgtgtca tatgttcagt gaccaatatt
     1681 tagataaaga agaaaatagc aaaattatgg atgttgtgtt ccagtggctt acaacaggag
     1741 acatccacct caaccagatt gatgctgagg acccagagat ttccgactac acgatggtac
     1801 cagacacagc caccctctca gagcagctcc gagtgtgtct gcaggagggc gatgagaacc
     1861 cccgtgactt taccaccctc tttgacctgt ccatttacca gctggacacc acctgcctgc
     1921 ccaaggtcat caaggctcac gaggaactaa atgtgaaaca tgaaccactt cagctcgtcc
     1981 aacctcagtt tgagatgccg ctgccagccc ttcagcctgc ggtcttccct ccgagtttcc
     2041 gggagttgcc gccccctccc ctggagctgt ttgacctaga tgaaacattc tcctctgaga
     2101 aggcgcgcct ggctcagatc accaacaagt gtactgatga agacctggaa ttctatgtca
     2161 ggaagtgtgg cgacattctt ggagtaacaa gcaaacttcc aaaggatcaa caggatgcca
     2221 aacatatcct tgaacacatc ttcttccaag tggtggagtt caagaaactg aaccaggagg
     2281 cccactgagg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     2341 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     2401 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2461 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2521 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     2581 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2641 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2701 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2761 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2821 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2881 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2941 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     3001 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3061 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3121 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     3181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3241 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3301 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3361 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3421 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3481 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     3541 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3601 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3661 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     3721 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3781 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3841 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3901 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3961 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4021 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     4081 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4141 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4201 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4261 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4321 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     4381 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     4441 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     4501 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     4561 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     4621 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     4681 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4741 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     4801 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4861 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4921 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4981 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5041 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5101 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5161 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5221 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5281 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5341 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5401 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5461 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5521 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5581 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
     5641 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     5701 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     5761 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     5821 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     5881 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     5941 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catg
//
