LOCUS       sequence-252107-        6792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of mouse Cep83.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Ye X, Zeng H, Ning G, Reiter JF, Liu A
  TITLE     C2cd3 is critical for centriolar distal appendage assembly and 
            ciliary vesicle docking in mammals.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2164-9. doi: 
            10.1073/pnas.1318737111. Epub 2014 Jan 27.
  PUBMED    24469809
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1318737111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-11- 11"; volume: "111"; issue: "6"; pages: "2164-9"
COMMENT     Sequence Label: pEGFPC1-Cep83
FEATURES             Location/Qualifiers
     source          1..6792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(294..315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(555..574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     902..923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3216..3337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3253..3272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3307..3326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3344..3799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3481..3502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3693..3712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3826..3930
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3932..4289
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3932..4123
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3955..3975)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4140..4275
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4324..5118
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4324..5118
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4378..4397)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4988..5007
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5306..5325)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5350..5397
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5726..6314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6215..6234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6489..6792
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      421 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      841 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      961 acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac gacacatttc
     1021 ctagcctatt tccacctgga ggagatagta ggctgaatcc tgagcctgag ttccaaaata
     1081 tgttaattga tgaaagggta cgctgtgaac atcataaaca taattatcag gctctgaaaa
     1141 ttgaacacaa aaggttgcag gaagaatatg taaaatcaca aaatgaactt aaacgtgtat
     1201 taattgaaaa gcaagcaagc caggaaaaat tccaactgct ccttgaagac ttaaggggag
     1261 aattagtaga gaaagctaga gacatagaaa aaatgaaact gcaggtacta acaccacaaa
     1321 aattggaatt ggtaaaagcc caactacaac aagaattaga agctccaatg cgagaacgtt
     1381 ttcggactct tgatgaagaa gtggaaaggt acagagctga gtataacaag ctgcgctacg
     1441 agtatacatt tctcaagtca gagtttgaac accagaaaga agagtttact cgggtttcag
     1501 aagaagagaa aatgaaatac aagtcagagg ttgcacgact ggagaaggac aaagaggagc
     1561 tacataacca gctgcttagt gttgatccca cgagagacag caaacgaatg gagcaacttg
     1621 ttcgagaaaa aacccatttg cttcagaaat tgaaaagttt agaggctgaa gtagcagaat
     1681 taagggctga gaaagaaaat tctggtgctc aggtagaaaa tgtccaaaga atacaggtga
     1741 ggcagttggc tgagatgcag gctacactca gatccttgga ggctgaaaag cagtcagcta
     1801 aactacaagc tgagcgttta gaaaaagaac tacaatcaag caatgaacag aatacctgct
     1861 taatcagcaa actgcataga gctgaccgag aaatcagcac actggccagt gaagtgaaag
     1921 agcttaaaca tgcaaacaaa ctagaaataa ctgacatcaa actggaggca gcaagagcta
     1981 agagtgagct cgaaagagaa aggaataaga tccaaagtga acttgatgga ttgcagtcag
     2041 acaatgaaat tctcaagtca actgttgaac accacaaagc gctcttagtg gaaaaagatc
     2101 gtgaattaat acgtaaagta caagctgcca aggaagaagg ttaccagaaa ctcatggtct
     2161 tacaagatga aaagctagaa cttgagaaca gattatcaga cttagagaag atgaaggtgg
     2221 agcgtgatgt ctggaggcag tcggaaaagg agcagtgtga ggagaagctg cgggcctccc
     2281 agatggcaga ggaggcagcc cgcagggagc tgcagagcac taggttaaaa cttcaacaac
     2341 aaattgtgaa taccgaaaaa gcagaaaaag aaaaacttga aaattctgaa ctgaaacagc
     2401 aaatcagtca tttgcagatc caagtgacct cactgacaca gtcggagaac gacttactga
     2461 attcaaacca catgctgaag gacatggtgg agagactaaa acaagaatgc cgaaatttaa
     2521 ggagccaagc tgagaaagca caactagacg ttgaaaagac actggaggaa aaacagatcc
     2581 agtggttgga agaaaagcac aagctccatg agcgaatcac agacagagaa gaaaagtata
     2641 accaagccaa ggaaaagcta cagcgcgcag cgactgctca gaaaaagaga aaatctcttc
     2701 atgagaacaa attgaagaga ctacaggaga aagtagaagt cttggaggca aagaaagaag
     2761 aactagaaac agaaaaccag gtgctaaata gacaaaatgt tccatttgaa gaatatacaa
     2821 ggcttcaaaa acgactaaag gacatacaga gaagacataa tgaatttcga agtctaattt
     2881 tggttcctaa catgcctcca acagcatcga ttagtcctgc taacttccag tccgcagtga
     2941 cagttcctgg agcggaactc tcttttcccc ctcatctgca ggaggaacaa catcaaagag
     3001 agctctctct ccttcgcaaa agactggaag aactggagac tacacaaaga aaacaactgg
     3061 aggagcttgg gtctcctggg gagtgaggat ccaccggatc tagataactg atcataatca
     3121 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     3181 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     3241 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3301 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat
     3361 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     3421 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     3481 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3541 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3601 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     3661 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     3721 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     3781 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3841 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3901 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     3961 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4021 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4081 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4141 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4201 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4261 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     4321 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     4381 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     4441 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4501 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     4561 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     4621 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     4681 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     4741 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     4801 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     4861 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     4921 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     4981 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5041 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5101 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     5161 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5221 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5281 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     5341 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     5401 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     5461 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     5521 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     5581 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5641 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6421 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     6481 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     6541 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     6601 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6661 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6721 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     6781 gctattacca tg
//
