LOCUS       sequence-252109-       11663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of mouse C2cd3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11663)
  AUTHORS   Hoover AN, Wynkoop A, Zeng H, Jia J, Niswander LA, Liu A
  TITLE     C2cd3 is required for cilia formation and Hedgehog signaling in 
            mouse.
  JOURNAL   Development. 2008 Dec;135(24):4049-58. doi: 10.1242/dev.029835. Epub
            2008 Nov 12.
  PUBMED    19004860
REFERENCE   2  (bases 1 to 11663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.029835"; journalName: "Development"; date: "2008-12"; 
            volume: "135"; issue: "24"; pages: "4049-58"
COMMENT     Sequence Label: pEGFPC3-C2cd3
FEATURES             Location/Qualifiers
     source          1..11663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(294..315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(555..574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     902..923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8087..8208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8124..8143)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8178..8197
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8215..8670)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8352..8373)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8564..8583
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8697..8801
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8803..9160
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8803..8994
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8826..8846)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9011..9146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9195..9989
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9195..9989
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9249..9268)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9859..9878
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10177..10196)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10221..10268
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10597..11185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11086..11105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        11360..11663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      421 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      841 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      961 acaagtactc agatctcgag aaacaacgga aaggccaagg gcctgggggc ggccgtgggc
     1021 gcaaaaagag aggtttgagt gacatttcac cgtctacaag cctgccacct ctggttgaag
     1081 ggcagttacg ctgctttctc aaactcacta ttaataaggt tgtatggaag attgcgaagc
     1141 ctcctacttc tgtacttgtc cgagtaaggt ggtggggaga aacatccgat ggaaccctct
     1201 tttgtcccag agatgcatta cagactgagc caaaagctgt gagaacaact acccgttacg
     1261 ggattcgctg tggtccaaag cagtttacct cgtatctgac agatatggct gtgctggtgt
     1321 tggaagtaat caccaagttt gatcatcttc ctgttggcag agttcagatc agtggacttg
     1381 cccagctgtc tccaacccat cagatcaatg ggttttttac cattgtttca ccagcatcga
     1441 agaaacttgg agaacttcag gtctcgcttg ccctggagcc tctgtcagaa acttatgaca
     1501 gctacaaacc tcttcctgcc actgaagtga caaaaaatgt gcttttatct gagcgagaac
     1561 tcagagagaa tactgaatcc agcaacaccc agtctatgat cccgtcaagg tcttgtaggg
     1621 gacctgccat caaaattgat gggaaagaat tagcaggcca cagcagcagg tcaaccacgc
     1681 caaggggaaa ggaccacttg tactttgcag agaactccga tgcagtaaag ggttctttgt
     1741 gtggactaca gcagcacctt aatcaaggga cgaatgtaga gactataact ctgaggggca
     1801 aagccccaca gaagcagctg tccctcctga acagctctga gtttcagcca caaattagta
     1861 cagtcgccaa gagtcacagt gactcatgca tcctgtcttc aaacaccccc cctgcaaagg
     1921 accttctttc agctctgtta gaacaaggca ataagctgcg gaatgccatg ttgatttctg
     1981 caatgaactc caacccagat actagtatgc tgcttgacaa agtccctcct cctatgacag
     2041 aagctattcc cagatcgtca gcactgaact catctgagaa tcactttaaa ggccacagtg
     2101 cagaccacct tctccccttg gcggacaccg gagctataca gctgctgttg ggcagtgctg
     2161 aattgtccca aggtcatttc tggaatggat taggctctcc tccagattcc ccgactcctg
     2221 ggagtgatga gtactgcagc agtgacctga atgatcctca gtatgaccag agtctgctgg
     2281 agaacttgtt ttacacagtg cctaaatctg atgtcggcac cagtgagctc cccagtgaag
     2341 atgatggagt ggaaccttct agaacaatga accagtccaa agcctctggc agatctaagg
     2401 ttgtagagtc aaaagagcag aagcagaaga gggcagctgt caagaaaagc agaaacccaa
     2461 ttgaccagca agagctctca agaactccag gacatacccc agcgatgtca ctaagtgtgg
     2521 atagactggc tcttctgggt agagtacatt cggtcagaat catcgttgaa accatgggag
     2581 tccctccaga tagtccccat atgacaccaa gcaggaagaa ctttgccggg aaaccaccaa
     2641 agccaacagc agcaaaaaag cgcactttct ttgtggagta tcactttcct gtgggctttt
     2701 ccaaaagtgg actgggaaag acagctttga tcactgaggt tgttagactt gcctccagta
     2761 aaatcaccga tggagtggtg aaattccagc agcgatttgt gtgtccagta gagtttggtg
     2821 gcccgatgat agagcactgg tgggattcta acctcatctt ccaaatttat gcaaagaaaa
     2881 ccccacagaa aaagccagaa gtcattggat ctgcatctct ccctttgcga gctgtcattc
     2941 agtcagagct gctttctttc agtagccagc ttccagtgca acaggaaaat ggtctgtctt
     3001 cacttggccc cctaaaggtc accatggagc ttgttttagg ccacaaggat ttcactggaa
     3061 tcagtgctaa gttaagtagc agcacccaac ctgctccagt ttctgctgct acaagttcag
     3121 atacgatact accagagaca ggccaggata ccgcctgtac cagaaatcca cagagctcaa
     3181 ataaaattca cgaggaaacc acaaagaaaa cacagaattt agtgctcccc gaccaagagt
     3241 cagcaaactc tgtagcctca aactcttcaa tcttcatggc tgtgccatct tgcaatttag
     3301 tccatcaaat aaatggctca aacaaagaaa gcggtttgct gttacatgtg ctgttgatgg
     3361 tgccagatgg gaaagatttt gtttttggag aacgggagaa acaaccatca tgcaatgttt
     3421 atttgaattg caagcttttc agcacagaag aagtcaccag atctgtcgtc tcgtggggca
     3481 cagcacagcc tgtctttaat ttttctcagg tgatccctgt ctctctgact tccaaatgcc
     3541 tggagagact taagaacaat gtgatgataa ttgaaacttg gaacaaggtg cggagcccag
     3601 gacaggacaa gctgctcggg ctggtgaaac tgccactcca tcagttttac atgtcattca
     3661 aagaccccaa gatttctcgc ctgctgcttg atgccaggta cccagttgtt gctgttgaca
     3721 gctatatgcc tgtgattgat gtgttctcgg gccaccaaaa tgggagtctt cgagtctttt
     3781 tagccatggg ttcttcagct cagataatga tgctgcaaag attaaagaat gaagaaggaa
     3841 cactccctcc cttcagtcct aggccagccc atttcctgga ccagccacct gtagcatctg
     3901 ttgctatgcc agaaaaacaa ggaacccggt tgatggagca ccactttgag ttctgtgtgg
     3961 cgatggttaa agggctcatg ccccttcagg caacagtctg gggagaagca gattgctacg
     4021 tccagtacta ctttccattt caagactccc aacccagtgt gctccaagga cccgacttcc
     4081 ttgaaaatgg aattactctg aagcccttta gaacttcaac cacactgtgt gtcccagatc
     4141 ccgtctttaa tagtgaacac catcactctc tcctgttgcc gactgacgtt ccagtgcaaa
     4201 ggctcctgct gagtgccttc tcctcccagg ggctggtgcc tggaggaggc gtccagtttg
     4261 aagtctggtg cagatactat tatcctaacg taagggacca gatggttgcc aaaggaacct
     4321 tgccattatc aagggtctgt gccatggtaa ccatgcagta ccgtgaagat gtgggaatgc
     4381 agagctttaa tctcccatta acctccagac ttgagcacag caaagaatta aagaaccagt
     4441 catcaggttt cctggatgtg ggtttgaggt accggcgtag cccaagaaca gcagagggaa
     4501 ttcttgctgc ccgagctgtc tccatctcag tccatattat cagagcctgt ggtcttcagg
     4561 cagcagccaa ggcgttggct gagcaggaac ccgctttaca gtttagcgcc accgttggag
     4621 tcaatgcttc tgtcaccgcc catctctcat ttctgcccaa gggggagcag cgccaaaccc
     4681 gccctgtggc ctgctccttc tgccctgagt tctcccacca cattgagttc ccgtgtaacc
     4741 tggtgactca gcactgtagt ggagaagcct gcttcctggc agagctgttg gagtttgcag
     4801 aaattatttt tgctatctat catgaaaata ccaaatcagt cagtgatata accagtatcc
     4861 agtcgtgcaa ggattatctg cttggaatag taaaggttcc aacgaaagat ctgctggtca
     4921 aaagatctgg gatcacagga tggtatcctg tcattttacc agaagacaaa ggcctgcctc
     4981 aggacctgga cttgatgcag aagattgtag gaggcttgga gctttctgtt tccttcgcac
     5041 atccgggaga tagagagagg gtactggaag ctgctgagct gctgggctgg agctttgaga
     5101 gcatcccaaa ggatcttgtc aagaaagaag aagaggtgcc agccactgtc accatctcta
     5161 ccccaaggct ctggcttcct atccactgtg tgctgcttgc tggtcatatg aacattcata
     5221 agaatacata ttgctacctt cgctacaagc tgtacaatca agaagccttt tggactcctc
     5281 tcaggaaacc taaggagtct acaaacaaaa accaggtcct gattactttc aaggcatcca
     5341 aaagagcaga agtcactagg agccagtccc tgctttggta cttcagggaa gagaagttag
     5401 agatccaagt gtggcgagcc tatggcaacg acaatttaga gaggccacac cagactgaca
     5461 gctggattgg ctcagcctat gtagacctgt ccagacttgg ggaaaaatct ccaaggacac
     5521 tgactatcag tggggtgtat cccctgtttg gaagaaatgc ttccgacctc tctggagctg
     5581 ctctgcgcat tcatgtgctt ctttccccgc tttccccaca cactgaacct gctcgggagc
     5641 tggactccat ggactgcagc agccacagtg agtctgagca gcaccccaga aagagcgatg
     5701 cgctccagct ctctccaccc catgtgctcc agacgtctcc cacctccacc caggtccacg
     5761 gcaacagtgc tgcagcccaa gtgtgccccg cccaggaggg ccctcctgag ttggctggga
     5821 cttttgcagt cagcatcctg gtagagagag ccatgcatct gagcttgaaa gggagcccct
     5881 tgacagaccg gaaagtatct gtacccagtt gctgtgtatc ctttgccaca gccactgagc
     5941 tgtctcctgt ctacactcac gtggttgaaa acacggattc ccccatctgg ggttttcacc
     6001 agcaggcaag gctttcaaaa gagctgcttc tggacccaca tcaaaccctg gtcttcaaag
     6061 tgtggcataa aggagatgaa gagagggtgg ttggctttgc ctcggtggac ctctccccac
     6121 tactgtctgg ctttcagttt atctgtggct ggtacaatat tacagacttc agtggagagt
     6181 gccaagggca aatcaaagtt gctatttcac ctatggagag cctgatgcac ctaaaagaag
     6241 aaagacaagc aagacgtggg atagatactc caggagcact gattccattg tttagcgccc
     6301 tttccttccc tgcctctgct ggatgcgatg cattccctag gcccattgca aggcacgtcg
     6361 aaggccagct tgctcacaca tctccaaagg aggacggtct ctcctctcct gcaagaaatg
     6421 gtgccatcag aagccaggcc gcacgccatg aagagcatgt gcagaacatc cgccgctttc
     6481 acgagtccct gcagcacggg gaggcagtcc tgacaagtga tgagaagctg accacagcgc
     6541 cttcgtcctc ccacacctcc atcctgactt ctctcaggaa gaatctgagt gagctagatg
     6601 agatccagaa gtacttcagc cagaagctca gcaagccctt cctgcccttc tcctctcaga
     6661 gctcgcctgc tgtctcacag agccaggaga gccagaggga ccctgtggct gcaggaaccg
     6721 gccgccagga ccccgagaac cagtgcatcc tggagaagtc caatcacctg gtgtcgcaag
     6781 tcagctcctt gatctcagat ctgcagactc tcaccagagg ttcacaagca gctttgactt
     6841 ctcagcaagc caggagtaga agcagagcag tcaccaccat cccagatgct cagggcaccg
     6901 aagctgctgg agaaggaagc accacactgg aggagccttt ggcaggagct atagaggcga
     6961 gcacagattc cctgcctccc cctgttgaag agccttccaa gggaggaggg atgctccatg
     7021 agtcgctgga gcagacgatg cccatcacga gggtgcagag catcgatgac acggaggtgg
     7081 gcccagacta cagtgatgaa gactatgagg aagacattat tgagcccagg accctaaatg
     7141 aaataaccac agtgacagac agaaccagcc cctggtccag tttcatgtca gacatgagtg
     7201 aggtccttag ccctcagccc actgaggtac aaagggaagg cccctcatgt cccccagaac
     7261 ctttccccag agaggagctg aaggtcaaaa gtagcccgca aaaggcagtc agcccccagc
     7321 ctgcacaggg ctcccctagc caaagtggtg tttgtgaggg tggagcctat aagatcgagg
     7381 ttgaagacct tgcctcagcc aagcctcagc ctgtcccaag cctaacattc tcagaagccc
     7441 aagagggcag cgattctgtt ggatggcgtg catcacagat aaatcaggtc aggaagccca
     7501 tgccagagat gctggctgag agtgaggctt tctccagtga gttcagtgac tcttctgaaa
     7561 gctttgagac gtttcctcta cacctaccct cccaaagcaa aagagaagac tacaaggact
     7621 cccctgctgt gaggcagaag caggtaccaa ctggatcaga ggtgtccaca aggcagacgc
     7681 tccttctccc agaacctgtt gtggtgccca acttcttttt gcctccccaa caactggagg
     7741 cctctctccg catgatctca cactctccag gtttgccacc agcagccaca actgaccagg
     7801 acaagagtga agccaccagg ggagcccttg cccagaggcc ttgtcgcccc agaccttact
     7861 ccatccctcc caaccttcct gaggaagaga ctcgcaggat tgcacggata ttttcttccc
     7921 agtattccaa aaagactgag gaaaccccgc gggcccggga tccaccggat ctagataact
     7981 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     8041 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     8101 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8161 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg
     8221 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     8281 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     8341 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     8401 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     8461 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8521 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8581 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8641 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     8701 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8761 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     8821 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     8881 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8941 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9001 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9061 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9121 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     9181 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     9241 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     9301 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     9361 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     9421 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     9481 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     9541 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     9601 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     9661 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     9721 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     9781 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     9841 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     9901 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     9961 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
    10021 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
    10081 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    10141 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
    10201 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
    10261 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
    10321 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
    10381 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
    10441 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10501 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10561 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10621 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10681 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10741 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10801 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10861 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10921 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10981 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11041 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11101 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11161 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    11221 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    11281 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
    11341 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
    11401 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
    11461 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
    11521 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
    11581 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
    11641 tattagtcat cgctattacc atg
//
