LOCUS       sequence-25215-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  TITLE     pTREtight with high copy E. coli origin
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTREtight2
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    190..271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(227..246)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     374..396
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(434..452)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        520..624
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agctcggtac ccggggnncc tctagtcagc tgacgcgtgc tagcgcggcc gcatcgataa
       61 gcttgtcgac gatatctcta gaggatcata atcagccata ccacatttgt agaggtttta
      121 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      181 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      241 aatttcacaa ataaagcatt tttttcactg cctcgagagt taagccagcc ccgacacccg
      301 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
      361 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
      421 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      481 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      601 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      661 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgct
//
