LOCUS       sequence-252171-        2833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses asPink 
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: asPink chromoprotein
FEATURES             Location/Qualifiers
     source          1..2833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        69..103
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    832..852
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      853..910
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(934..951)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1105..1693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1185..1204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1876..1970)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1991..2650)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2565..2584
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2812..2830
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga gaaagaggag
      121 aaatactaga tggcgagctt cctgaaaaag acgatgccgt tcaaaaccac gattgaaggc
      181 acggtcaacg gtcactactt taaatgtacg ggtaaaggcg aaggtaaccc gttcgaaggc
      241 acccaggaaa tgaaaattga agtcatcgaa ggcggtccgc tgccgtttgc gtttcatatt
      301 ctgagcacgt cttgcatgta cggctcaaag accttcatca agtacgtgtc gggtatcccg
      361 gattacttta aacagagctt cccggaaggc tttacctggg aacgtaccac gacctatgaa
      421 gatggcggtt tcctgacggc gcaccaagac acctctctgg atggtgactg tctggtgtac
      481 aaagttaaga ttctgggcaa caattttccg gccgatggtc cggttatgca gaacaaagcg
      541 ggccgttggg aaccggctac ggaaatcgtc tatgaagtgg acggcgttct gcgcggtcaa
      601 tccctgatgg ccctgaaatg cccgggcggt cgtcatctga cctgtcatct gcacacgacc
      661 tatcgtagca aaaaaccggc gagcgccctg aaaatgccgg gctttcattt cgaagatcac
      721 cgcattgaaa tcatggaaga agtcgaaaaa ggtaagtgct acaagcagta cgaagcagca
      781 gtgggtcgct attgtgatgc ggcaccgagc aagctgggtc ataattaata atactagtag
      841 cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
      901 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
      961 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1021 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1081 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
     1141 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1201 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1261 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1321 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1381 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1441 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1501 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1561 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1621 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1861 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1921 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1981 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     2041 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     2101 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     2161 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     2221 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     2281 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2341 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2401 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     2461 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     2521 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     2581 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     2641 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     2701 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga
     2761 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     2821 atcacgaggc aga
//
