LOCUS       sequence-252185-       11711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GUCY2C G549C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-GUCY2C-G549C
FEATURES             Location/Qualifiers
     source          1..11711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4449..4473)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4481..4499
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4524..4543)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4525..4543)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4549..4569)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4623..4664
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4685..5252
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4702..5254
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4846..4863)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5073..5092
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5266..5865
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5266..5285)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5762..5782
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5879..6467
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5932..5952)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6350..6361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6542..6775
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6894..6912)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6980..7084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7085..7153,7154..7945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7303..7322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8116..8704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8605..8624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8858..8875
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9115..9136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9151..9156,9157..9174,9175..9181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9189..9205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9194..9216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9213..9229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9213..9229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9279..9298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9490..10123
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10170..10295
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10792..11025
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11210..11254
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11547..11664
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac
     1201 aagtttgtac aaaaaagcag gcaccatgaa gacgttgctg ttggacttgg ctttgtggtc
     1261 actgctcttc cagcccgggt ggctgtcctt tagttcccag gtgagtcaga actgccacaa
     1321 tggcagctat gaaatcagcg tcctgatgat gggcaactca gcctttgcag agcccctgaa
     1381 aaacttggaa gatgcggtga atgaggggct ggaaatagtg agaggacgtc tgcaaaatgc
     1441 tggcctaaat gtgactgtga acgctacttt catgtattcg gatggtctga ttcataactc
     1501 aggcgactgc cggagtagca cctgtgaagg cctcgaccta ctcaggaaaa tttcaaatgc
     1561 acaacggatg ggctgtgtcc tcatagggcc ctcatgtaca tactccacct tccagatgta
     1621 ccttgacaca gaattgagct accccatgat ctcagctgga agttttggat tgtcatgtga
     1681 ctataaagaa accttaacca ggctgatgtc tccagctaga aagttgatgt acttcttggt
     1741 taacttttgg aaaaccaacg atctgccctt caaaacttat tcctggagca cttcgtatgt
     1801 ttacaagaat ggtacagaaa ctgaggactg tttctggtac cttaatgctc tggaggctag
     1861 cgtttcctat ttctcccacg aactcggctt taaggtggtg ttaagacaag ataaggagtt
     1921 tcaggatatc ttaatggacc acaacaggaa aagcaatgtg attattatgt gtggtggtcc
     1981 agagttcctc tacaagctga agggtgaccg agcagtggct gaagacattg tcattattct
     2041 agtggatctt ttcaatgacc agtacttgga ggacaatgtc acagcccctg actatatgaa
     2101 aaatgtcctt gttctgacgc tgtctcctgg gaattccctt ctaaatagct ctttctccag
     2161 gaatctatca ccaacaaaac gagactttgc tcttgcctat ttgaatggaa tcctgctctt
     2221 tggacatatg ctgaagatat ttcttgaaaa tggagaaaat attaccaccc ccaaatttgc
     2281 tcatgctttc aggaatctca cttttgaagg gtatgacggt ccagtgacct tggatgactg
     2341 gggggatgtt gacagtacca tggtgcttct gtatacctct gtggacacca agaaatacaa
     2401 ggttcttttg acctatgata cccacgtaaa taagacctat cctgtggata tgagccccac
     2461 attcacttgg aagaactcta aacttcctaa tgatattaca ggccggggcc ctcagatcct
     2521 gatgattgca gtcttcaccc tcactggagc tgtggtgctg ctcctgctcg tcgctctcct
     2581 gatgctcaga aaatatagaa aagattatga acttcgtcag aaaaaatggt cccacattcc
     2641 tcctgaaaat atctttcctc tggagaccaa tgagaccaat catgttagcc tcaagatcga
     2701 tgatgacaaa agacgagata caatccagag actacgacag tgcaaatacg acaaaaagcg
     2761 agtgattctc aaagatctca agcacaatga tggtaatttc actgaaaaac agaagataga
     2821 attgaacaag ttgcttcaga ttgactatta caacctgacc aagttctact gcacagtgaa
     2881 acttgatacc atgatcttcg gggtgataga atactgtgag agaggatccc tccgggaagt
     2941 tttaaatgac acaatttcct accctgatgg cacattcatg gattgggagt ttaagatctc
     3001 tgtcttgtat gacattgcta agggaatgtc atatctgcac tccagtaaga cagaagtcca
     3061 tggtcgtctg aaatctacca actgcgtagt ggacagtaga atggtggtga agatcactga
     3121 ttttggctgc aattccattt tacctccaaa aaaggacctg tggacagctc cagagcacct
     3181 ccgccaagcc aacatctctc agaaaggaga tgtgtacagc tatgggatca tcgcacagga
     3241 gatcattctg cggaaagaaa ccttctacac tttgagctgt cgggaccgga atgagaagat
     3301 tttcagagtg gaaaattcca atggaatgaa acccttccgc ccagatttat tcttggaaac
     3361 agcagaggaa aaagagctag aagtgtacct acttgtaaaa aactgttggg aggaagatcc
     3421 agaaaagaga ccagatttca aaaaaattga gactacactt gccaagatat ttggactttt
     3481 tcatgaccaa aaaaatgaaa gctatatgga taccttgatc cgacgtctac agctatattc
     3541 tcgaaacctg gaacatctgg tagaggaaag gacacagctg tacaaggcag agagggacag
     3601 ggctgacaga cttaacttta tgttgcttcc aaggctagtg gtaaagtctc tgaaggagaa
     3661 aggctttgtg gagccggaac tatatgagga agttacaatc tacttcagtg acattgtagg
     3721 tttcactact atctgcaaat acagcacccc catggaagtg gtggacatgc ttaatgacat
     3781 ctataagagt tttgaccaca ttgttgatca tcatgatgtc tacaaggtgg aaaccatcgg
     3841 tgatgcgtac atggtggcta gtggtttgcc taagagaaat ggcaatcggc atgcaataga
     3901 cattgccaag atggccttgg aaatcctcag cttcatgggg acctttgagc tggagcatct
     3961 tcctggcctc ccaatatgga ttcgcattgg agttcactct ggtccctgtg ctgctggagt
     4021 tgtgggaatc aagatgcctc gttattgtct atttggagat acggtcaaca cagcctctag
     4081 gatggaatcc actggcctcc ctttgagaat tcacgtgagt ggctccacca tagccatcct
     4141 gaagagaact gagtgccagt tcctttatga agtgagagga gaaacatact taaagggaag
     4201 aggaaatgag actacctact ggctgactgg gatgaaggac cagaaattca acctgccaac
     4261 ccctcctact gtggagaatc aacagcgttt gcaagcagaa ttttcagaca tgattgccaa
     4321 ctctttacag aaaagacagg cagcagggat aagaagccaa aaacccagac gggtagccag
     4381 ctataaaaaa ggcactctgg aatacttgca gctgaatacc acagacaagg agagcaccta
     4441 tttttagaac ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaaggt
     4501 ttgttgttaa gtatgtgatt tagccctata gtgagtcgta ttaccaccca acttttctat
     4561 acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
     4621 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat
     4681 gagatccctc ccccccccnt aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4741 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4801 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     4861 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     4921 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     4981 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     5041 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     5101 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     5161 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     5221 acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag
     5281 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5341 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5401 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5461 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5521 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5581 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5641 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5701 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5761 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5821 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa
     5881 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5941 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6001 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6061 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6121 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6181 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6241 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6301 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6361 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6421 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     6481 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6541 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6601 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6661 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6721 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     6781 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6841 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     6901 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6961 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7021 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7081 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7141 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7201 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7261 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7321 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7381 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7441 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7501 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7561 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7621 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7681 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7741 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7801 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7861 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7921 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7981 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8041 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8101 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8161 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8221 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8281 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8341 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8401 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8461 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8521 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8581 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8641 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8701 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8761 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8821 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8881 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8941 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9001 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     9061 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     9241 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9301 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9361 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     9421 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     9481 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     9541 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     9601 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     9661 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     9721 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     9781 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     9841 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     9901 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     9961 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    10021 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    10081 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    10141 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    10201 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    10261 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    10321 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    10381 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    10441 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    10501 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    10561 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    10621 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    10681 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    10741 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    10801 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    10861 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    10921 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    10981 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11041 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11101 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    11161 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11221 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11281 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11341 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11401 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    11461 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
    11521 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    11581 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    11641 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
    11701 actagtcgtg a
//
