LOCUS       sequence-252220-        5949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  modular integrative vector for the assembly of DNA fragments and 
            expression in Streptomyces species.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Aubry C, Pernodet JL, Lautru S
  TITLE     A set of modular and integrative vectors for synthetic biology in 
            Streptomyces.
  JOURNAL   Appl Environ Microbiol. 2019 Jun 7. pii: AEM.00485-19. doi: 
            10.1128/AEM.00485-19.
  PUBMED    31175189
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2019 Jun 7. pii: AEM.00485-19. doi: 
            10.1128/AEM.00485-19."
COMMENT     Sequence Label: pOSV812
FEATURES             Location/Qualifiers
     source          1..5949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             142..153
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      845..916
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      932..959
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    3339..3372
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     oriT            3573..3682
                     /note="incP origin of transfer"
     CDS             complement(4080..4883)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSRMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4191..4210)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4801..4820
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(5136..5153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5270..5815)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5350..5369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5916..5949
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atgcatgcgg ccgctgctag cgttttttga tctcaatcaa taaagtcgcc tatcttttca
       61 gcaacaaaac ttgattaaca tcaattttgg tatgaccaat gcaccattca tgttattctc
      121 aatagcgaag aactactaga gaaagaggag aaatactaga tgagtgtgat cgctaaacaa
      181 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc
      241 gatggaaaag gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc
      301 ggacctctgc catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca
      361 ttcaccaagt accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat
      421 acatgggaga ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc
      481 agcatccaag gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc
      541 aatggacctg tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt
      601 gcacgagatg gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt
      661 cactatttgt gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg
      721 tatcactatg ttgaccgcaa actggatgta accaatcaca acaaggatta cacttcggtt
      781 gagcagtgtg aaatttccat tgcacgcaaa cctgtggtcg cctaataata ctagagccag
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      961 ttatatacta gtagcggccg cttaaggtcg acccgtctga cgcgtgtggg gagcacgccg
     1021 gcccgctcgg tagacagcac tctacgcatt gtggctgctg tgcacatcac tctttcgagc
     1081 gacgtgatcc gcaggattcc gtggggatgt ggcatctatg tggcatccgg gtacgacaac
     1141 ggccccccgt tcgaatggaa cggggggccg ttgactccct gacctgcgat gcaggttgtg
     1201 cccccggcag gattcgaacc tgcgacaccc gctttaggag agaacgtaaa gcgatcatga
     1261 gcctcagtga gtctgagtga gtctcagtga gcctgtcgcc ataggggctt cacctgcata
     1321 aatgagtttc agtgagtctc ggtgagtctc agtgaggggg tgttgtggca tccctgtggc
     1381 atctgcgtgg catccggcgt cacaccgccc gcagcgttcg aacaccctcg gtctcgggag
     1441 ccatcgccgc ccgcaccttc gccgccacgt cggccggcga atgctggtac aaccacgtca
     1501 ccttcgaccc tcgctcgtga cccatcacgg tctgcgtgtc cttctcgtca atgcccacat
     1561 ccttgagcca cgtggcgaac acgtggcgaa ggtcgtgcac ccgcggccac cactccatgc
     1621 ggccggtgtc ccggttcttc acctcgcggg cgaggcctgc agccttgatt gcaggtatcc
     1681 atgtgcgccg gaagttgtgc cgggtcagga cgccgccctg tgggccgcgg aacacgagct
     1741 catccggcgc gaggtcgtac gggtcctcgc caatcggggt gatcgtggga gtcggccgcc
     1801 accgggtgac catggtgcgg atcgcgtcct cggcctgcgg cgtgagcggg accgtgcgga
     1861 acccggcgtt gctcttcggc gcaggcttcc ggaacagctt gccttcgtcg tcactgagca
     1921 cttccttcac cttgaggtgg tgggcgtcca ggtcgacgtt ctcccagcgc agcgccgtcg
     1981 cctcgcccca ccggagaccg gtgtgttcaa ggaagacgac gagcggccgg tagtacatcg
     2041 gcaggtgact gacgatcagc gcgcactgcg cgcggtcggg cggcatcagt tcgtccggat
     2101 gcttcgccgg cgcctcgcca atgtccaggt cggccgccgg gttgaacggg atacgccggc
     2161 cgtccttgac ggcggcccgg agcatcgagt tcagcacctc atgaaccttc ttccgggtgt
     2221 ggtagccctt cacctccttc gtgatccacg cctgcagctc gatgtactcc aagtcgcaga
     2281 gacgccactg cccccacttc ggctcgatgt gcgcggccca gttcgaccgc ttccggttgg
     2341 cggtcgtgac tgctcggtcc ggctgcgcct cccaccacag tttccaccac tgggacaggg
     2401 tgatctcgcc acgcttcggg tcggcgtacg tccgttcgcg gacctgcgtg cggatcttgt
     2461 cgaggtgcgc ctcggctgcc ttcttaccgc cgtccttgat cgcaaagcat ttctgcttct
     2521 ggttgccggc cgggtcgcgg taacgcgcct gccacgagcc ggtgcagtcg cgtcggcggt
     2581 tccgctctcc gtacttctcg ggcgggtact tctccatgca cagctggcag ccgcagctct
     2641 tcgccctcaa ctgccgtggg ttgttcgtcg ctctaggcgc catgggtcac ctgtccgctc
     2701 ctccgctgtt cgggaacgcg gggcaggatg tgtacggctg ccccacacca gcagactgca
     2761 ccgtccgggg gctgctctac agccagttct tcaaggacgg ctcgaagggc tacgagggaa
     2821 gcatcccggg ggagggtggc aggcagggtg accgtgtttc cagcggcgtc gtacggccgg
     2881 aagttgagcg ggtcgagagg cgcgaagcgg acacgaatgc acatgggtat cccccgggca
     2941 ggcaggcgac gtttgaccgt cggccatggg ggagggcagg ccgctcgacc gaccgtaccc
     3001 ccttgtaggt gaatatgcga ccactctgcg tgcaccaagt gcccgaggaa tcacggggag
     3061 tggtggagtc cattttcgtt agacccgcga ggcaggccga cggttgtacg ggcgtcggct
     3121 actgcttgtt ggactcgttc agcgcgctca tctcgatcag cttggaacgc tgctgctcct
     3181 cggtgagacc acggaacagg gtgagcaggc gttcctcggc gtcctcgtcg agcgggcccg
     3241 gcgcggcccg gcgggccgcg gcgaaaatgc gctcttcggt gaacttcggg tacgcctcat
     3301 ggagggcgcg aagctttcgc gagctcgaga cctgcaggga agttcctatt ctctagaaag
     3361 tataggaact tcgtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta
     3421 ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg
     3481 aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat
     3541 ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc
     3601 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg
     3661 gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg
     3721 aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc
     3781 tataatgacc ccgaagcagg gttatgcagc ggaaaagggt accacgtcgc ggtgagttca
     3841 ggctttttca tatctcattg cccccggacg agcgtctgct ccgccattcg ccgtccgccg
     3901 tgccaatcgg atcagccgtc caaatgcggg attttcgtta gtcggaggcc aaacggcatt
     3961 gagcgtcagc atatcatcag cgagctgaag aaagacaatc cccgatccgc tccacgtgtt
     4021 gccccagcaa tcagcgcgac cttgcccctc caacgtcatc tcgttctccg ctcatgagct
     4081 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     4141 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     4201 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     4261 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     4321 acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg
     4381 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     4441 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     4501 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     4561 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     4621 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     4681 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     4741 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     4801 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     4861 tcttgttcaa tcatgcgaga cattgcactc caccgctgat gacatcagtc gatcatagca
     4921 cgatcaacgg cactgttgca aatagtcggt ggtgataaac ttatcatccc cttttgctga
     4981 tggagctgca catgaaccgg atcccctagc ggagtgtata ctggcttact atgttggcac
     5041 tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt
     5101 cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg
     5161 gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat
     5221 gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc
     5281 tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga
     5341 caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc
     5401 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     5461 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     5521 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
     5581 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
     5641 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
     5701 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
     5761 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     5821 tcatcttatt aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt
     5881 agcacctgaa gtcagcccca tacgatataa gttgtgaagt tcctattctc tagaaagtat
     5941 aggaacttc
//
