LOCUS       sequence-252223-       12169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of JAG1 E228K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-JAG1-E228K
FEATURES             Location/Qualifiers
     source          1..12169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4884..4908)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4961..5002
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5023..5590
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5040..5592
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5184..5201)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5411..5430
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5604..5606,5607..5609,5610..6323)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5604..5606,5607..5609,5610..6320)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5649..5670)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5910..5929)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6257..6278
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6337..6925
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6390..6410)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6808..6819)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7000..7233
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7352..7370)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7438..7542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7543..7611,7612..8403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7761..7780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8574..9162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9063..9082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9316..9333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9573..9594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9609..9614,9615..9632,9633..9639)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9647..9663
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9652..9674
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9671..9687
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9671..9687
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9737..9756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9948..10581
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10628..10753
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11250..11483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11668..11712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12005..12122
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac
     1201 aagtttgtac aaaaaagcag gcaccatgcg ttccccacgg acgcgcggcc ggtccgggcg
     1261 ccccctaagc ctcctgctcg ccctgctctg tgccctgcga gccaaggtgt gtggggcctc
     1321 gggtcagttc gagttggaga tcctgtccat gcagaacgtg aacggggagc tgcagaacgg
     1381 gaactgctgc ggcggcgccc ggaacccggg agaccgcaag tgcacccgcg acgagtgtga
     1441 cacatacttc aaagtgtgcc tcaaggagta tcagtcccgc gtcacggccg gggggccctg
     1501 cagcttcggc tcagggtcca cgcctgtcat cgggggcaac accttcaacc tcaaggccag
     1561 ccgcggcaac gaccgcaacc gcatcgtgct gcctttcagt ttcgcctggc cgaggtccta
     1621 tacgttgctt gtggaggcgt gggattccag taatgacacc gttcaacctg acagtattat
     1681 tgaaaaggct tctcactcgg gcatgatcaa ccccagccgg cagtggcaga cgctgaagca
     1741 gaacacgggc gttgcccact ttgagtatca gatccgcgtg acctgtgatg actactacta
     1801 tggctttggc tgcaataagt tctgccgccc cagagatgac ttctttggac actatgcctg
     1861 tgaccagaat ggcaacaaaa cttgcatgga aggctggatg ggccccaaat gtaacagagc
     1921 tatttgccga caaggctgca gtcctaagca tgggtcttgc aaactcccag gtgactgcag
     1981 gtgccagtac ggctggcaag gcctgtactg tgataagtgc atcccacacc cgggatgcgt
     2041 ccacggcatc tgtaatgagc cctggcagtg cctctgtgag accaactggg gcggccagct
     2101 ctgtgacaaa gatctcaatt actgtgggac tcatcagccg tgtctcaacg ggggaacttg
     2161 tagcaacaca ggccctgaca aatatcagtg ttcctgccct gaggggtatt caggacccaa
     2221 ctgtgaaatt gctgagcacg cctgcctctc tgatccctgt cacaacagag gcagctgtaa
     2281 ggagacctcc ctgggctttg agtgtgagtg ttccccaggc tggaccggcc ccacatgctc
     2341 tacaaacatt gatgactgtt ctcctaataa ctgttcccac gggggcacct gccaggacct
     2401 ggttaacgga tttaagtgtg tgtgcccccc acagtggact gggaaaacgt gccagttaga
     2461 tgcaaatgaa tgtgaggcca aaccttgtgt aaacgccaaa tcctgtaaga atctcattgc
     2521 cagctactac tgcgactgtc ttcccggctg gatgggtcag aattgtgaca taaatattaa
     2581 tgactgcctt ggccagtgtc agaatgacgc ctcctgtcgg gatttggtta atggttatcg
     2641 ctgtatctgt ccacctggct atgcaggcga tcactgtgag agagacatcg atgaatgtgc
     2701 cagcaacccc tgtttgaatg ggggtcactg tcagaatgaa atcaacagat tccagtgtct
     2761 gtgtcccact ggtttctctg gaaacctctg tcagctggac atcgattatt gtgagcctaa
     2821 tccctgccag aacggtgccc agtgctacaa ccgtgccagt gactatttct gtaagtgccc
     2881 cgaggactat gagggcaaga actgctcaca cctgaaagac cactgccgca cgaccccctg
     2941 tgaagtgatt gacagctgca cagtggccat ggcttccaac gacacacctg aaggggtgcg
     3001 gtatatttcc tccaacgtct gtggtcctca cgggaagtgc aagagtcagt cgggaggcaa
     3061 attcacctgt gactgtaaca aaggcttcac gggaacatac tgccatgaaa atattaatga
     3121 ctgtgagagc aacccttgta gaaacggtgg cacttgcatc gatggtgtca actcctacaa
     3181 gtgcatctgt agtgacggct gggagggggc ctactgtgaa accaatatta atgactgcag
     3241 ccagaacccc tgccacaatg ggggcacgtg tcgcgacctg gtcaatgact tctactgtga
     3301 ctgtaaaaat gggtggaaag gaaagacctg ccactcacgt gacagtcagt gtgatgaggc
     3361 cacgtgcaac aacggtggca cctgctatga tgagggggat gcttttaagt gcatgtgtcc
     3421 tggcggctgg gaaggaacaa cctgtaacat agcccgaaac agtagctgcc tgcccaaccc
     3481 ctgccataat gggggcacat gtgtggtcaa cggcgagtcc tttacgtgcg tctgcaagga
     3541 aggctgggag gggcccatct gtgctcagaa taccaatgac tgcagccctc atccctgtta
     3601 caacagcggc acctgtgtgg atggagacaa ctggtaccgg tgcgaatgtg ccccgggttt
     3661 tgctgggccc gactgcagaa taaacatcaa tgaatgccag tcttcacctt gtgcctttgg
     3721 agcgacctgt gtggatgaga tcaatggcta ccggtgtgtc tgccctccag ggcacagtgg
     3781 tgccaagtgc caggaagttt cagggagacc ttgcatcacc atggggagtg tgataccaga
     3841 tggggccaaa tgggatgatg actgtaatac ctgccagtgc ctgaatggac ggatcgcctg
     3901 ctcaaaggtc tggtgtggcc ctcgaccttg cctgctccac aaagggcaca gcgagtgccc
     3961 cagcgggcag agctgcatcc ccatcctgga cgaccagtgc ttcgtccacc cctgcactgg
     4021 tgtgggcgag tgtcggtctt ccagtctcca gccggtgaag acaaagtgca cctctgactc
     4081 ctattaccag gataactgtg cgaacatcac atttaccttt aacaaggaga tgatgtcacc
     4141 aggtcttact acggagcaca tttgcagtga attgaggaat ttgaatattt tgaagaatgt
     4201 ttccgctgaa tattcaatct acatcgcttg cgagccttcc ccttcagcga acaatgaaat
     4261 acatgtggcc atttctgctg aagatatacg ggatgatggg aacccgatca aggaaatcac
     4321 tgacaaaata atcgatcttg ttagtaaacg tgatggaaac agctcgctga ttgctgccgt
     4381 tgcagaagta agagttcaga ggcggcctct gaagaacaga acagatttcc ttgttccctt
     4441 gctgagctct gtcttaactg tggcttggat ctgttgcttg gtgacggcct tctactggtg
     4501 cctgcggaag cggcggaagc cgggcagcca cacacactca gcctctgagg acaacaccac
     4561 caacaacgtg cgggagcagc tgaaccagat caaaaacccc attgagaaac atggggccaa
     4621 cacggtcccc atcaaggatt atgagaacaa gaactccaaa atgtctaaaa taaggacaca
     4681 caattctgaa gtagaagagg acgacatgga caaacaccag cagaaagccc ggtttgccaa
     4741 gcagccggcg tacacgctgg tagacagaga agagaagccc cccaacggca cgccgacaaa
     4801 acacccaaac tggacaaaca aacaggacaa cagagacttg gaaagtgccc agagcttaaa
     4861 ccgaatggag tacatcgtat agaacccagc tttcttgtac aaagtggttg atatccagca
     4921 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
     4981 tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa
     5041 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     5101 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5161 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5221 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5281 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5341 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     5401 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     5461 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     5521 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5581 acgatgataa tatggccaca catatggtga gcaagggcga ggagctgttc accggggtgg
     5641 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     5701 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     5761 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     5821 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     5881 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     5941 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     6001 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     6061 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     6121 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     6181 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     6241 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     6301 gcatggacga gctgtacaag taaatcgata gatcctaatc aacctctgga ttacaaaatt
     6361 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6421 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6481 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6541 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6601 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6661 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6721 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6781 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6841 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6901 atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag
     6961 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7021 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7081 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7141 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7201 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7261 agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg
     7321 aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7381 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     7801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt
     9421 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9481 gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc
     9541 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9601 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9661 caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt
     9721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9781 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     9841 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     9901 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
     9961 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
    10021 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
    10081 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
    10141 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
    10201 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
    10261 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
    10321 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
    10381 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    10441 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10501 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10561 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    10621 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    10681 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    10741 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    10801 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    10861 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    10921 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    10981 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    11041 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
    11101 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    11161 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    11221 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    11281 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    11341 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    11401 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    11461 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    11521 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    11581 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    11641 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    11701 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    11761 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    11821 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    11881 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    11941 attcgattag tgaacggatc tcgacggtat cgccgaattc acaaatggca gtattcatcc
    12001 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    12061 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    12121 ttcgggttta ttacagggac agcagagatc cagtttggac tagtcgtga
//
