LOCUS       sequence-25224-e         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Zhou Q, Brown J, Kanarek A, Rajagopal J, Melton DA
  TITLE     In vivo reprogramming of adult pancreatic exocrine cells to 
            beta-cells.
  JOURNAL   Nature. 2008 Oct 2;455(7213):627-32. doi: 10.1038/nature07314. Epub 
            2008 Aug 27.
  PUBMED    18754011
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07314"; journalName: "Nature"; date: "2008-10-2- 2"; 
            volume: "455"; issue: "7213"; pages: "627-32"
COMMENT     Sequence Label: pAd nGFP
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             140..160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             164..184
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             188..208
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(263..284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(524..543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acnantnnnn
       61 ntagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctc
      121 caccatggtg cacgtggatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa
      181 ggtagatcca aaaaagaaga gaaaggtaca cgtgtccatg gtgagcaagg gcgaggagct
      241 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      301 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      361 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      421 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      481 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      541 gacccgcgcc gaggtgaagt tcgagggcga caccct
//
