LOCUS       sequence-252246-        4387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for SodA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PsodA-cfp
FEATURES             Location/Qualifiers
     source          1..4387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     149..168
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             549..560
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1333..1404
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1420..1447
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1978..2928)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3690..3784)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3815..4387,1..222))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3926..3945)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
       61 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
      121 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
      181 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
      241 tgtctcttga tcagatcatg atcccctgcg ccatcagatc cttggcggca agaaagccat
      301 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga
      361 cgtcggtgcc taatgagtga gctaacttac attaattgcg ttgcgcggca atcacggcat
      421 taagtgggtg atttgcttca catctcgggc attttcctgc aaaaccatac ccttacgaaa
      481 agtacggcat tgataatcat tttcaatatc atttaattaa ctataatgaa ccaaggatcc
      541 ttaactttaa agaggagaaa ggtaccgcat gactagcaaa agaagcaaag gtgaagaact
      601 gttcactggt gttgttccaa ttctggttga actggatggt gatgttaatg gtcacaaatt
      661 ttctgtctct ggtgagggtg aaggtgatgc aacctacggt aaactgaccc tgaaatttat
      721 ttgcactact ggtaaactgc ctgttccgtg gccaaccctg gtcactactc tgacttgggg
      781 tgttcaatgc tttgctcgtt acccagatca catgaaacag catgactttt tcaagtctgc
      841 catgccggaa ggttatgttc aggaacgtac tatctttttc aaagatgacg gtaactacaa
      901 gacccgtgct gaagtcaagt ttgaaggtga taccctggtt aatcgtatcg agctgaaagg
      961 tattgatttt aaagaagatg gtaacattct gggtcacaaa ctggaataca acgctatttc
     1021 tgataatgta tacatcactg ctgacaaaca aaagaatggt atcaaagcta atttcaaaat
     1081 tcgtcacaac attgaagatg gtagcgttca actggcagac cattatcaac aaaatactcc
     1141 aattggcgat ggccctgtcc tgctgccaga caaccattac ctgtccaccc aatctcgtct
     1201 gtctaaagat ccgaacgaaa agcgcgatca catggtcctg ctggagtttg taaccgctgc
     1261 tggtattacc ctgggcatgg atgaactgta taaataatag ggatccaaac tcgagtaagg
     1321 atctccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1381 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1441 ttctgcgttt atacctaggg tacgggtttt gctgcccgca aacgggctgt tctggtgttg
     1501 ctagtttgtt atcagaatcg cagatccggc ttcagccggt ttgccggctg aaagcgctat
     1561 ttcttccaga attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc
     1621 ggcagctttg attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga
     1681 gctgtaacaa gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt
     1741 tcacctgttc tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg
     1801 tgaacagctt tgaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca
     1861 ccgttttcat ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt
     1921 ctacttttgt ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca
     1981 gatccttccg tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca
     2041 tgagaacgaa ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa
     2101 actggtgagc tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg
     2161 taggtaggaa tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt
     2221 gttctcaagt tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt
     2281 atcagtcggg cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc
     2341 tttacttatt ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc
     2401 aagcattaac atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt
     2461 cttttgtgtt agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa
     2521 agacttaaca tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa
     2581 tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca
     2641 ctggaaaatc tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag
     2701 ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg
     2761 agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt
     2821 ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata
     2881 gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg
     2941 gtctaggtga ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt
     3001 ccttttcccg ggtgatctgg gtatctgtaa attctgctag acctttgctg gaaaacttgt
     3061 aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat
     3121 tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc
     3181 cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca
     3241 aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct
     3301 cgcaagctcg agcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc
     3361 gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta
     3421 aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa
     3481 agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac
     3541 tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc
     3601 cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc
     3661 ttacccgtct tactgtccct agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     3721 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     3781 agtccaagcg agctctcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     3841 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     3901 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     3961 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     4021 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     4081 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     4141 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     4201 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     4261 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     4321 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     4381 aaggaac
//
