LOCUS       sequence-252247-        2918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter for SoxS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: PsoxS-cfp  (ColE1)
FEATURES             Location/Qualifiers
     source          1..2918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             216..227
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1000..1071
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1087..1114
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1145..1733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1225..1244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1816..1910)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1941..2735)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2052..2071)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2662..2681
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccacgtttat atcgccgctg attgccgctg ttacggatac tggtaatcaa ccctttactt
       61 tcatagaaat gcagcgccga taccgccaca ccgctgcgtt tcgccacttc gccgggggtt
      121 agcagcgctt taatgcgggg taatttcttt tccataaatc gctttacctc aagttaactt
      181 gaggaattat actccccaac aggatcctta actttaaaga ggagaaaggt accgcatgac
      241 tagcaaaaga agcaaaggtg aagaactgtt cactggtgtt gttccaattc tggttgaact
      301 ggatggtgat gttaatggtc acaaattttc tgtctctggt gagggtgaag gtgatgcaac
      361 ctacggtaaa ctgaccctga aatttatttg cactactggt aaactgcctg ttccgtggcc
      421 aaccctggtc actactctga cttggggtgt tcaatgcttt gctcgttacc cagatcacat
      481 gaaacagcat gactttttca agtctgccat gccggaaggt tatgttcagg aacgtactat
      541 ctttttcaaa gatgacggta actacaagac ccgtgctgaa gtcaagtttg aaggtgatac
      601 cctggttaat cgtatcgagc tgaaaggtat tgattttaaa gaagatggta acattctggg
      661 tcacaaactg gaatacaacg ctatttctga taatgtatac atcactgctg acaaacaaaa
      721 gaatggtatc aaagctaatt tcaaaattcg tcacaacatt gaagatggta gcgttcaact
      781 ggcagaccat tatcaacaaa atactccaat tggcgatggc cctgtcctgc tgccagacaa
      841 ccattacctg tccacccaat ctcgtctgtc taaagatccg aacgaaaagc gcgatcacat
      901 ggtcctgctg gagtttgtaa ccgctgctgg tattaccctg ggcatggatg aactgtataa
      961 ataataggga tccaaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag
     1021 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1081 cacactggct caccttcggg tgggcctttc tgcgtttata cctaggggcc gcgttgctgg
     1141 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1201 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1261 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1321 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1381 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1441 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1501 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1561 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     1621 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1681 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1741 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1801 tggtcatgag cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1861 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1921 ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     1981 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     2041 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     2101 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     2161 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg
     2221 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     2281 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     2341 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     2401 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     2461 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     2521 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg
     2581 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     2641 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     2701 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     2761 tgatcagatc atgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
     2821 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cgacgtcggt
     2881 gcctaatgag tgagctaact tacattaatt gcgttgcg
//
