LOCUS       sequence-252252-        2737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fluorescent reporter with no promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Rossi NA, El Meouche I, Dunlop MJ
  TITLE     Forecasting cell fate during antibiotic exposure using stochastic 
            gene expression.
  JOURNAL   Commun Biol. 2019 Jul 11;2:259. doi: 10.1038/s42003-019-0509-0. 
            eCollection 2019.
  PUBMED    31312728
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-07-11"; volume: "2:259. doi"; pages: " 
            10.1038/s42003-019-0509-0. eCollection 2019"
COMMENT     Sequence Label: No promoter  (ColE1)
FEATURES             Location/Qualifiers
     source          1..2737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             15..26
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      799..870
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      886..913
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(944..1532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1024..1043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1615..1709)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1740..2534)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1851..1870)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2461..2480
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ggatccttaa ctttaaagag gagaaaggta ccgcatgact agcaaaagaa gcaaaggtga
       61 agaactgttc actggtgttg ttccaattct ggttgaactg gatggtgatg ttaatggtca
      121 caaattttct gtctctggtg agggtgaagg tgatgcaacc tacggtaaac tgaccctgaa
      181 atttatttgc actactggta aactgcctgt tccgtggcca accctggtca ctactctgac
      241 ttggggtgtt caatgctttg ctcgttaccc agatcacatg aaacagcatg actttttcaa
      301 gtctgccatg ccggaaggtt atgttcagga acgtactatc tttttcaaag atgacggtaa
      361 ctacaagacc cgtgctgaag tcaagtttga aggtgatacc ctggttaatc gtatcgagct
      421 gaaaggtatt gattttaaag aagatggtaa cattctgggt cacaaactgg aatacaacgc
      481 tatttctgat aatgtataca tcactgctga caaacaaaag aatggtatca aagctaattt
      541 caaaattcgt cacaacattg aagatggtag cgttcaactg gcagaccatt atcaacaaaa
      601 tactccaatt ggcgatggcc ctgtcctgct gccagacaac cattacctgt ccacccaatc
      661 tcgtctgtct aaagatccga acgaaaagcg cgatcacatg gtcctgctgg agtttgtaac
      721 cgctgctggt attaccctgg gcatggatga actgtataaa taatagggat ccaaactcga
      781 gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      901 gggcctttct gcgtttatac ctaggggccg cgttgctggc gtttttccat aggctccgcc
      961 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1021 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1081 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1141 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1201 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1261 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1321 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1381 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1441 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1501 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1561 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgagc ttggattctc
     1621 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1681 ggtcattact ggatctatca acaggagtcc aagcgagctc tcgaacccca gagtcccgct
     1741 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     1801 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     1861 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     1921 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     1981 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2041 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2101 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2161 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2221 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2281 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2341 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2401 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2461 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2521 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatca tgatcccctg
     2581 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
     2641 ttaccagagg gcgccccagc tggcaattcc gacgtcggtg cctaatgagt gagctaactt
     2701 acattaattg cgttgcgcgt tgactacctg ggtggtc
//
