LOCUS       sequence-252307-        9878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet 
            normalization in a PiggyBac destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 9878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0507
FEATURES             Location/Qualifiers
     source          1..9878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        540..743
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     694..714
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      765..774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             771..1799
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             771..1799
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    1817..2024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2231..2293
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(2344..2362)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3125..3144
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3209..3228
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3442..4030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3931..3950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4142..4161
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4142..4160
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4190..4224
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        4747..5246
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4782..4805)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5179..5198
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5279..5299
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5315..5986
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     CDS             5315..5986
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     polyA_signal    6013..6237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6485..6788
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6790..7065
                     /label=chicken beta-actin promoter
     intron          7066..8082
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8006..8028
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8090..8109
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             8143..8520
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     primer_bind     complement(9394..9413)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9459..9514
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9513..9532)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gttacagaga tgttacgaac cctgaacagg ttgactatta attgtgtctg cttgatgtgg
       61 acaccaggtg gcgctggaca tcagatttgg agaggcagtt gtctagggaa ccgggctctg
      121 tgccagcgca ggaggcaggc tggctctcct attccaggga tgctcatcca ggaaggaaag
      181 gttgcatgct ggacacacta accttacagc ggtgggctct atggggtacc tacgtaggta
      241 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      301 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      361 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      421 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      481 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      541 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      601 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      661 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      721 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatagccacc atgggtaaaa
      781 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtgt
      841 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      901 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      961 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     1021 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     1081 tgcctgaaac cgaactgccc gctgttctgc aaccggtcgc ggaggccatg gatgcgatcg
     1141 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     1201 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     1261 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     1321 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     1381 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     1441 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     1501 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     1561 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     1621 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     1681 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     1741 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataac
     1801 tcgagcgacg cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     1861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     1921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     1981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2041 agtatcgata ctagttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt
     2101 tttttaataa ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag
     2161 tgtaaatata ataaaactta atatctattc aaattaataa ataaacctcg atatacagac
     2221 cgataaaaca catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat
     2281 tatctttcta gggttaatct agtatacgcg tatgcggccg cttaattaat ccagcttttg
     2341 ttccctttag tgagggttaa ttccgagcgg ccgcgtgtta caaccaatta accaattctg
     2401 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     2461 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     2521 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     2581 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     2641 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
     2701 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     2761 gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     2821 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     2881 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     2941 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     3001 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     3061 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     3121 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     3181 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     3241 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     3301 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     3361 atcagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4081 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgccaa
     4141 ttaatacgac tcactatagg gcgaattggg gcgcgccatt ctagattaac cctagaaaga
     4201 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
     4261 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
     4321 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
     4381 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
     4441 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcaa
     4501 ctagaatgct agcatgggcc cagatctcca tgggccctga attcgcatct agactgaact
     4561 ggccgataat tgcagacgag gagcatcgcc cttccccggc cctcaggtaa gaggaccaaa
     4621 taccgtagcc gtttccaatt tcagtccttt agcgccacct ggtgctaact actctatcac
     4681 gcttttatcc aataactacc tttgtaaatt tcctttcaaa agttctggcc gggcgcggtg
     4741 gcaacggggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     4801 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     4861 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     4921 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     4981 tagcatgtct cactaggctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     5041 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     5101 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     5161 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     5221 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttgtcgacg ccactatgcc
     5281 caagaagaag aggaaggtgg aggacgccgg ttctgtgagc gtgatcaagc ccgagatgaa
     5341 gatcaagctg tgcatgaggg gcaccgtgaa cggccacaac ttcgtgatcg agggcgaggg
     5401 caagggcaac ccctacgagg gcacccagat cctggacctg aacgtgaccg agggcgcccc
     5461 cctgcccttc gcctacgaca tcctgaccac cgtgttccag tacggcaaca gggccttcac
     5521 caagtacccc gccgacatcc aggactactt caagcagacc ttccccgagg gctaccactg
     5581 ggagaggagc atgacctacg aggaccaggg catctgcacc gccaccagca acatcagcat
     5641 gaggggcgac tgcttcttct acgacatcag gttcgacggc accaacttcc cccccaacgg
     5701 ccccgtgatg cagaagaaga ccctgaagtg ggagcccagc accgagaaga tgtacgtgga
     5761 ggacggcgtg ctgaagggcg acgtgaacat gaggctgctg ctggagggcg gcggccacta
     5821 caggtgcgac ttcaagacca cctacaaggc caagaaggag gtgaggctgc ccgacgccca
     5881 caagatcgac cacaggatcg agatcctgaa gcacgacaag gactacaaca aggtgaagct
     5941 gtacgagaac gccgtggcca ggtactccat gctgcccagc caggccggat cctaactcga
     6001 gcgacgcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     6061 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     6121 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6181 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgggct
     6241 ggttctgtat gggcacagac aacctaacta atttcctggc cctcaaggtg acttgcttga
     6301 acttgccacc ttcgccacta gggggcagca ttggtttaca cagggtgaac aaacgttcaa
     6361 ttctaaacta atattctttg gtgggaaagt gtgttcattt tggtttgttt tgaagaagtc
     6421 acttcatacc tttgaactgg ggttaagctg gttaactccc aaaaacgcca tatatggagt
     6481 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     6541 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     6601 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     6661 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     6721 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     6781 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     6841 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     6901 gggggggggg gcgcgccagg cggggcgggg cggggggagg ggcggggcgg ggcgaggcgg
     6961 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
     7021 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
     7081 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
     7141 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     7201 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
     7261 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
     7321 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
     7381 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     7441 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     7501 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
     7561 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     7621 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     7681 ggggagggct cgggggaggg gcgcggcggc ccccggagtg ccggcggctg tcgaggcgcg
     7741 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     7801 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     7861 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     7921 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     7981 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     8041 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     8101 gttattgtgc tgtctcatca ttttggcaaa gaattcgcca ctatgccaga gccagcgaag
     8161 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
     8221 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg
     8281 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt
     8341 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
     8401 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
     8461 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
     8521 ggttctgtat caaaaggcga ggcggtcatt aaagagttca tgaggtttaa agtccatatg
     8581 gaaggaagca tgaatggtca cgagtttgag atagagggag agggtgaggg aaggccgtac
     8641 gaagggacgc aaactgcgaa gttgaaggta acgaaaggcg gccctctgcc attctcttgg
     8701 gacatccttt caccgcaatt tatgtacggc tctagggcct tcacaaaaca ccccgccgat
     8761 attccggatt actataaaca aagttttcct gagggcttca agtgggaaag agtaatgaac
     8821 ttcgaggatg ggggagctgt gacggttact caagacacta gcctggagga cggtactctt
     8881 atatacaagg taaaactaag agggacgaat tttccgccag acggccccgt gatgcaaaaa
     8941 aaaactatgg ggtgggaagc atccaccgag aggctgtacc ccgaagacgg ggtcctgaag
     9001 ggtgatatta aaatggcatt aagattaaaa gatggcggga gatacttagc tgacttcaag
     9061 actacatata aagccaaaaa acctgtacag atgcctggag cttacaatgt ggatcgtaag
     9121 ctggatatta catcccacaa cgaagattat accgtggtgg agcagtacga gcgttcagag
     9181 gggcgtcact ccaccggggg tatggatgaa ttatacaaga gcggtcttag aagcagggcg
     9241 caagcgtcaa attcagcggt agatggaaca gcaggtccag gtagtactgg cagcagagga
     9301 tcctaactcg agattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     9361 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     9421 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     9481 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     9541 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     9601 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     9661 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     9721 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     9781 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     9841 cctcgacctg cagcccaagc ttggcgtaat gctgagca
//
