LOCUS       sequence-252380-        5332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the 
            hSyn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Candemir E, Kollert L, Weissflog L, Geis M, Muller A, Post AM, 
            O'Leary A, Harro J, Reif A, Freudenberg F
  TITLE     Interaction of NOS1AP with the NOS-I PDZ domain: Implications for 
            schizophrenia-related alterations in dendritic morphology.
  JOURNAL   Eur Neuropsychopharmacol. 2016 Apr;26(4):741-55. doi: 
            10.1016/j.euroneuro.2016.01.008. Epub 2016 Jan 28.
  PUBMED    26861996
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.euroneuro.2016.01.008"; journalName: "Eur 
            Neuropsychopharmacol"; date: "2016-04"; volume: "26"; issue: "4"; 
            pages: "741-55"
COMMENT     Sequence Label: pAAV-hSyn-mCherry.3xFLAG-WPRE
FEATURES             Location/Qualifiers
     source          1..5332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      654..663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             660..1367
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(805..823)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1073..1092
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1374..1439
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1459..2047
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1512..1532)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1930..1941)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2079..2555
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2278..2297)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2595..2735
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2595..2724
                     /label=AAV2 ITR
     rep_origin      2810..3265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2897..2916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3107..3128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3282..3301)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3401..3423
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3461..3479)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3547..3651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3652..3720,3721..4512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3870..3889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4683..5271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5172..5191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc ctctagagtc gacgccacca
      661 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
      721 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      781 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
      841 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
      901 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      961 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     1021 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     1081 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     1141 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     1201 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     1261 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     1321 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggaa ttcgactaca
     1381 aagaccatga cggtgattat aaagatcatg atattgatta caaggatgac gatgacaagc
     1441 tagcataagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     1501 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     1561 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     1621 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     1681 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     1741 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     1801 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     1861 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     1921 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     1981 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2041 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     2101 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     2161 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     2221 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     2281 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     2341 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     2401 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     2461 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     2521 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     2581 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     2641 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     2701 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     2761 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     2821 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2881 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2941 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3001 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     3061 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3121 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     3181 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3241 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     3301 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3361 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3421 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     3481 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3541 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3601 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3661 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3721 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3781 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3841 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     3901 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3961 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4021 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4081 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4141 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4201 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4261 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4321 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4381 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4441 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4501 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4561 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4621 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4681 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4741 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4801 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     4861 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4921 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4981 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5041 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5101 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5161 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5221 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5281 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
