LOCUS       sequence-252446-        7645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Httex1 (without proline-rich region) fused to ymScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Albakri MB, Jiang Y, Genereaux J, Lajoie P
  TITLE     Polyglutamine toxicity assays highlight the advantages of mScarlet 
            for imaging in Saccharomyces cerevisiae.
  JOURNAL   F1000Res. 2018 Aug 10;7:1242. doi: 10.12688/f1000research.15829.2. 
            eCollection 2018.
  PUBMED    30631438
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2018-08-10"; volume: "7:1242. doi"; pages: " 
            10.12688/f1000research.15829.2. eCollection 2018"
COMMENT     Sequence Label: 68Q-ymScarlet
FEATURES             Location/Qualifiers
     source          1..7645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      872..1119
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(888..906)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(1137..1156)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1138..1156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1166..1183)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1166..1182)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1175..1197)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1323..1778
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1410..1429)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1620..1641
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2079..2486
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2487..3581
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(4075..4094)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4194..4216
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4254..4272)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    4314..4817
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        4854..4958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4959..5027,5028..5819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5177..5196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5990..6578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6479..6498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6732..6749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6866..6887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6902..6907,6908..6925,6926..6932)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6940..6956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6945..6967
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6964..6980
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6964..6980
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6999..7019
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7001..7019
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7038..7479
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    7038..7155
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     7450..7473
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             7506..7529
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acagcaacaa cagcagcaac agcaacaaca gcagcagcag caacaacagc agcaacagca
       61 acaacaacag cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca
      121 acagcagcaa cagcaacaac tgcagcccgg gggatccact agtgtttcta aaggtgaagc
      181 agttattaag gaattcatga gattcaaggt acacatggaa ggttctatga atggtcacga
      241 atttgaaatt gaaggtgaag gtgaaggtag accatatgaa ggtactcaaa ctgctaagtt
      301 gaaggttact aaaggtggtc cattgccatt ttcttgggat attttgtctc cacaattcat
      361 gtacggttct agagctttta caaaacatcc agcagatatt ccagattact acaagcaatc
      421 attcccagaa ggttttaaat gggaaagagt tatgaacttc gaagatggtg gtgcagttac
      481 tgttacacaa gatacttctt tggaagatgg tacattgatc tataaggtta agttgagagg
      541 tactaatttt ccaccagatg gtccagttat gcaaaagaaa actatgggtt gggaagcttc
      601 aacagaaaga ttgtacccag aagatggtgt tttgaagggt gacattaaga tggcattgag
      661 attgaaggat ggtggtagat atttggctga tttcaagact acatacaagg ctaagaaacc
      721 agttcaaatg ccaggtgctt acaacgttga tagaaagttg gatattactt ctcataatga
      781 agattacaca gttgttgaac aatatgaaag aagtgaaggt agacacagta caggtggtat
      841 ggatgaatta tacaaatgag tcgacctcga gtcatgtaat tagttatgtc acgcttacat
      901 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
      961 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
     1021 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
     1081 cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc aattcgccct
     1141 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1201 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1261 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1321 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1381 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1441 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1501 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1561 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1621 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1681 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1741 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     1801 acgcatctgt gcggtatttc acaccgcata tcgaccggtc gaggagaact tctagtatat
     1861 ctacatacct aatattattg ccttattaaa aatggaatcc caacaattac atcaaaatcc
     1921 acattctctt caaaatcaat tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta
     1981 tatttatagg ataattatac tctatttctc aacaagtaat tggttgtttg gccgagcggt
     2041 ctaaggcgcc tgattcaaga aatatcttga ccgcagttaa ctgtgggaat actcaggtat
     2101 cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca acataacgag
     2161 aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt
     2221 tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg
     2281 tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
     2341 catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
     2401 gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
     2461 atatatttca aggatatacc attctaatgt ctgcccctaa gaagatcgtc gttttgccag
     2521 gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg
     2581 atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg
     2641 atgctacagg tgttccactt ccagatgagg cgctggaagc ctccaagaag gctgatgccg
     2701 ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag
     2761 gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg
     2821 catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact
     2881 tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg
     2941 gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa
     3001 gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata
     3061 aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga
     3121 acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag
     3181 ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca
     3241 tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg
     3301 cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc
     3361 cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt
     3421 tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg
     3481 ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg
     3541 gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat
     3601 gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa
     3661 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
     3721 aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg
     3781 tgataaggaa aaagaattgc actttaacat taatattgac aaggaggagg gcaccacaca
     3841 aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc taatttatat attggaggat
     3901 tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
     3961 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
     4021 ttttactctg tcagaaacgg ccttaacgac gtagtcgata tggtgcactc tcagtacaat
     4081 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4141 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4201 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     4261 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagg acggatcgct
     4321 tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg gaatttactc
     4381 tgtgtttatt tatttttatg ttttgtattt ggattttaga aagtaaataa agaaggtaga
     4441 agagttacgg aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg
     4501 tactttacat atatatttat tagacaagaa aagcagatta aatagatata cattcgatta
     4561 acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat
     4621 gaaacaattc ggcattaata cctgagagca ggaagagcaa gataaaaggt agtatttgtt
     4681 ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt
     4741 ctttttttac tttctatttt taatttatat atttatatta aaaaatttaa attataatta
     4801 tttttatagc acgtgatgaa aaggacccag gtggcacttt tcggggaaat gtgcgcggaa
     4861 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4921 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5341 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5401 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5461 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5521 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5581 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5641 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5701 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5761 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5821 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6601 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6721 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6781 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6841 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6901 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6961 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7021 aacaaaagct ggagctccgg attagaggcc gccgagcggg tgacagccct ccgaaggaag
     7081 actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg
     7141 cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg
     7201 aaaaattggc agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca
     7261 taggatgata atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg
     7321 atgatttttg atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa
     7381 tactttcaac attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa
     7441 aaaattgtta atatacctct atactttaac gtcaaggaga aaaaaccccg gattctagag
     7501 ccatggacta caaggacgac gatgacaagc tggcgaccct ggaaaagcta atgaaggcct
     7561 tcgagtccct caaaagcttc caacagcagc aacagcaaca acagcagcaa cagcaacaac
     7621 agcagcaaca gcaacaacag cagca
//
