LOCUS       sequence-252575-       11687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA E109_I112delinsD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA-E109_I112delinsD
FEATURES             Location/Qualifiers
     source          1..11687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6647..6671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6679..6697
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6722..6741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6723..6741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6747..6767)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6821..6862
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6883..7450
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6900..7452
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7044..7061)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7271..7290
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7464..8063
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7464..7483)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7960..7980
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8077..8665
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8130..8150)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8548..8559)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8740..8973
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9092..9110)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9178..9282
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9283..9351,9352..10143)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9501..9520)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10314..10902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10803..10822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11056..11073
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11313..11334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11349..11354,11355..11372,11373..11379)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11387..11403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11392..11414
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11411..11427
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11411..11427
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11477..11496
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cctccaagac catcatcagg tgaactgtgg
     3481 ggcatccact tgatgccccc aagaatccta gtagaatgtt tactaccaaa tggaatgata
     3541 gtgactttag aatgcctccg tgaggctaca ttaataacca taaagcatga actatttaaa
     3601 gaagcaagaa aataccccct ccatcaactt cttcaagatg aatcttctta cattttcgta
     3661 agtgttactc aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac
     3721 cttcggcttt ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgacctcaat
     3781 cgagaaattg gttttgctat cggcatgcca gtgtgtgaat ttgatatggt taaagatcca
     3841 gaagtacagg acttccgaag aaatattctg aacgtttgta aagaagctgt ggatcttagg
     3901 gacctcaatt cacctcatag tagagcaatg tatgtctatc ctccaaatgt agaatcttca
     3961 ccagaattgc caaagcacat atataataaa ttagataaag ggcaaataat agtggtgatc
     4021 tgggtaatag tttctccaaa taatgacaag cagaagtata ctctgaaaat caaccatgac
     4081 tgtgtaccag aacaagtaat tgctgaagca atcaggaaaa aaactcgaag tatgttgcta
     4141 tcctctgaac aactaaaact ctgtgtttta gaatatcagg gcaagtatat tttaaaagtg
     4201 tgtggatgtg atgaatactt cctagaaaaa tatcctctga gtcagtataa gtatataaga
     4261 agctgtataa tgcttgggag gatgcccaat ttgatgttga tggctaaaga aagcctttat
     4321 tctcaactgc caatggactg ttttacaatg ccatcttatt ccagacgcat ttccacagct
     4381 acaccatata tgaatggaga aacatctaca aaatcccttt gggttataaa tagtgcactc
     4441 agaataaaaa ttctttgtgc aacctacgtg aatgtaaata ttcgagacat tgataagatc
     4501 tatgttcgaa caggtatcta ccatggagga gaacccttat gtgacaatgt gaacactcaa
     4561 agagtacctt gttccaatcc caggtggaat gaatggctga attatgatat atacattcct
     4621 gatcttcctc gtgctgctcg actttgcctt tccatttgct ctgttaaagg ccgaaagggt
     4681 gctaaagagg aacactgtcc attggcatgg ggaaatataa acttgtttga ttacacagac
     4741 actctagtat ctggaaaaat ggctttgaat ctttggccag tacctcatgg attagaagat
     4801 ttgctgaacc ctattggtgt tactggatca aatccaaata aagaaactcc atgcttagag
     4861 ttggagtttg actggttcag cagtgtggta aagttcccag atatgtcagt gattgaagag
     4921 catgccaatt ggtctgtatc ccgagaagca ggatttagct attcccacgc aggactgagt
     4981 aacagactag ctagagacaa tgaattaagg gaaaatgaca aagaacagct caaagcaatt
     5041 tctacacgag atcctctctc tgaaatcact gagcaggaga aagattttct atggagtcac
     5101 agacactatt gtgtaactat ccccgaaatt ctacccaaat tgcttctgtc tgttaaatgg
     5161 aattctagag atgaagtagc ccagatgtat tgcttggtaa aagattggcc tccaatcaaa
     5221 cctgaacagg ctatggaact tctggactgt aattacccag atcctatggt tcgaggtttt
     5281 gctgttcggt gcttggaaaa atatttaaca gatgacaaac tttctcagta tttaattcag
     5341 ctagtacagg tcctaaaata tgaacaatat ttggataact tgcttgtgag atttttactg
     5401 aagaaagcat tgactaatca aaggattggg cactttttct tttggcattt aaaatctgag
     5461 atgcacaata aaacagttag ccagaggttt ggcctgcttt tggagtccta ttgtcgtgca
     5521 tgtgggatgt atttgaagca cctgaatagg caagtcgagg caatggaaaa gctcattaac
     5581 ttaactgaca ttctcaaaca ggagaagaag gatgaaacac aaaaggtaca gatgaagttt
     5641 ttagttgagc aaatgaggcg accagatttc atggatgctc tacagggctt tctgtctcct
     5701 ctaaaccctg ctcatcaact aggaaacctc aggcttgaag agtgtcgaat tatgtcctct
     5761 gcaaaaaggc cactgtggtt gaattgggag aacccagaca tcatgtcaga gttactgttt
     5821 cagaacaatg agatcatctt taaaaatggg gatgatttac ggcaagatat gctaacactt
     5881 caaattattc gtattatgga aaatatctgg caaaatcaag gtcttgatct tcgaatgtta
     5941 ccttatggtt gtctgtcaat cggtgactgt gtgggactta ttgaggtggt gcgaaattct
     6001 cacactatta tgcaaattca gtgcaaaggc ggcttgaaag gtgcactgca gttcaacagc
     6061 cacacactac atcagtggct caaagacaag aacaaaggag aaatatatga tgcagccatt
     6121 gacctgttta cacgttcatg tgctggatac tgtgtagcta ccttcatttt gggaattgga
     6181 gatcgtcaca atagtaacat catggtgaaa gacgatggac aactgtttca tatagatttt
     6241 ggacactttt tggatcacaa gaagaaaaaa tttggttata aacgagaacg tgtgccattt
     6301 gttttgacac aggatttctt aatagtgatt agtaaaggag cccaagaatg cacaaagaca
     6361 agagaatttg agaggtttca ggagatgtgt tacaaggctt atctagctat tcgacagcat
     6421 gccaatctct tcataaatct tttctcaatg atgcttggct ctggaatgcc agaactacaa
     6481 tcttttgatg acattgcata cattcgaaag accctagcct tagataaaac tgagcaagag
     6541 gctttggagt atttcatgaa acaaatgaat gatgcacatc atggtggctg gacaacaaaa
     6601 atggattgga tcttccacac aattaaacag catgcattga actagaaccc agctttcttg
     6661 tacaaagtgg tgatatccaa ttaaccctca ctaaaggagt aagtgttaga tagtattgaa
     6721 tccctatagt gagtcgtatt accacccaac ttttctatac aaagtggttg atatccagca
     6781 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
     6841 tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa
     6901 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     6961 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     7021 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     7081 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     7141 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     7201 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     7261 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     7321 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     7381 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     7441 acgatgataa tatggccaca catatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     7501 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     7561 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     7621 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     7681 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     7741 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     7801 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     7861 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     7921 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     7981 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     8041 tgacccgcaa gcccggtgcc taaatcgata gatcctaatc aacctctgga ttacaaaatt
     8101 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8161 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8221 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8281 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8341 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8401 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8461 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     8521 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8581 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8641 atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag
     8701 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8761 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8821 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8881 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8941 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     9001 agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg
     9061 aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     9121 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     9181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     9241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9301 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9361 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9421 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9481 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     9541 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9601 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9661 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9721 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9781 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9841 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9901 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9961 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    10021 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    10081 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    10141 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    10201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    10261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10441 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10981 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    11041 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    11101 ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt
    11161 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    11221 gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc
    11281 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    11341 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    11401 caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt
    11461 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11521 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    11581 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    11641 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
