LOCUS       sequence-252762-        5055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing Ad5 Fiber gene flanked by homology arms for 
            insertion into the recombinant Ad5 vector IQ013 to generate tropism 
            modified vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, 
            Newgard CB
  TITLE     Creation of versatile cloning platforms for transgene expression and
            dCas9-based epigenome editing.
  JOURNAL   Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 
            10.1093/nar/gky1286.
  PUBMED    30590691
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286."
COMMENT     Sequence Label: IX001: pBS/Fiber
FEATURES             Location/Qualifiers
     source          1..5055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             675..701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     724..744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     5'UTR           2788..2830
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(2867..2887)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2867..2885)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2906..2922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2906..2922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2919..2941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2930..2946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2954..2960,2961..2978,2979..2984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2999..3020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3137..3154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3308..3896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3388..3407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4067..4858,4859..4927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4690..4709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4928..5032)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacgac
      661 ccaagcaggc tagttaccca tacgatgtgc cagattacgc tggaggcaga tcacacctaa
      721 gcacgcaaat gggcggtagg cgtgcgtctc acagcagcac ctccttgccc tcctcccacg
      781 tcgacggctc tggtattgca gcttcctcct ggctgcaaac tttctccaca atctaaatgg
      841 aatgtcagtt tcctcctgtt cctgtccatc cgcacccact atcttcatgt tgttgcagat
      901 gaagcgcgca agaccgtctg aagatacctt caaccccgtg tatccatatg acacggaaac
      961 cggtcctcca actgtgcctt ttcttactcc tccctttgta tcccccaatg ggtttcaaga
     1021 gagtccccct ggggtactct ctttgcgcct atccgaacct ctagttacct ccaatggcat
     1081 gcttgcgctc aaaatgggca acggcctctc tctggacgag gccggcaacc ttacctccca
     1141 aaatgtaacc actgtgagcc cacctctcaa aaaaaccaag tcaaacataa acctggaaat
     1201 atctgcaccc ctcacagtta cctcagaagc cctaactgtg gctgccgccg cacctctaat
     1261 ggtcgcgggc aacacactca ccatgcaatc acaggccccg ctaaccgtgc acgactccaa
     1321 acttagcatt gccacccaag gacccctcac agtgtcagaa ggaaagctag ccctgcaaac
     1381 atcaggcccc ctcaccacca ccgatagcag tacccttact atcactgcct caccccctct
     1441 aactactgcc actggtagct tgggcattga cttgaaagag cccatttata cacaaaatgg
     1501 aaaactagga ctaaagtacg gggctccttt gcatgtaaca gacgacctaa acactttgac
     1561 cgtagcaact ggtccaggtg tgactattaa taatacttcc ttgcaaacta aagttactgg
     1621 agccttgggt tttgattcac aaggcaatat gcaacttaat gtagcaggag gactaaggat
     1681 tgattctcaa aacagacgcc ttatacttga tgttagttat ccgtttgatg ctcaaaacca
     1741 actaaatcta agactaggac agggccctct ttttataaac tcagcccaca acttggatat
     1801 taactacaac aaaggccttt acttgtttac agcttcaaac aattccaaaa agcttgaggt
     1861 taacctaagc actgccaagg ggttgatgtt tgacgctaca gccatagcca ttaatgcagg
     1921 agatgggctt gaatttggtt cacctaatgc accaaacaca aatcccctca aaacaaaaat
     1981 tggccatggc ctagaatttg attcaaacaa ggctatggtt cctaaactag gaactggcct
     2041 tagttttgac agcacaggtg ccattacagt aggaaacaaa aataatgata agctaacttt
     2101 gtggaccaca ccagctccat ctcctaactg tagactaaat gcagagaaag atgctaaact
     2161 cactttggtc ttaacaaaat gtggcagtca aatacttgct acagtttcag ttttggctgt
     2221 taaaggcagt ttggctccaa tatctggaac agttcaaagt gctcatctta ttataagatt
     2281 tgacgaaaat ggagtgctac taaacaattc cttcctggac ccagaatatt ggaactttag
     2341 aaatggagat cttactgaag gcacagccta tacaaacgct gttggattta tgcctaacct
     2401 atcagcttat ccaaaatctc acggtaaaac tgccaaaagt aacattgtca gtcaagttta
     2461 cttaaacgga gacaaaacta aacctgtaac actaaccatt acactaaacg gtacacagga
     2521 aacaggagac acaactccaa gtgcatactc tatgtcattt tcatgggact ggtctggcca
     2581 caactacatt aatgaaatat ttgccacatc ctcttacact ttttcataca ttgcccaaga
     2641 ataaagaatc gtttgtgtta tgtttcaacg tgtttatttt tcaattgcag aaaatttcga
     2701 atcatttttc attcagtagt atagccccag agacgggtaa gcctatccct aaccctctcc
     2761 tcggtctcga ttgtagaata cgagcttctt gttctttttg cagaagcaca gaataaacgc
     2821 tcaactttgg cagctgtgaa cattctacga gctccagctt ttgttccctt tagtgagggt
     2881 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2941 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3001 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3061 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3481 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3721 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4681 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5041 cccgaaaagt gccac
//
