LOCUS       sequence-253014-        9606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPGK1_FUSN_Citrine _PixE_TCYC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Zhao EM, Suek N, Wilson MZ, Dine E, Pannucci NL, Gitai Z, Avalos JL,
            Toettcher JE
  TITLE     Light-based control of metabolic flux through assembly of synthetic 
            organelles.
  JOURNAL   Nat Chem Biol. 2019 Jun;15(6):589-597. doi: 
            10.1038/s41589-019-0284-8. Epub 2019 May 13.
  PUBMED    31086330
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0284-8"; journalName: "Nat Chem Biol"; date: 
            "2019-06"; volume: "15"; issue: "6"; pages: "589-597"
COMMENT     Sequence Label: EZ-L498
FEATURES             Location/Qualifiers
     source          1..9606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(13..31)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(52..68)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(52..68)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(65..87)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    76..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(100..106,107..124,125..130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    145..166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(283..300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(454..1042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(534..553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1213..2004,2005..2073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1836..1855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2074..2178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2262..2280
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2318..2340)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2440..2459
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2815..3270)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2952..2973)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3164..3183
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3396..3418
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3410..3427
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3411..3427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3434..3453
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3434..3452
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5231..5242
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(5502..5504,5505..5507,5508..6218)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5547..5568)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5808..5827)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6155..6176
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      7392..7639
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7408..7426)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(9106..9293)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct
       61 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat
      121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcc tcggcgcgtg
     2221 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2281 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2341 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2401 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2461 tgagagtgca ccataccacc ttttcaattc atcatttttt ttttattctt ttttttgatt
     2521 tcggtttcct tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
     2581 ggagcacaga cttagattgg tatatatacg catattgggt cagcagtaca gaaccctcga
     2641 cgaagttgta agtggtggat cagatatcat cattgttggc agaggacttt tcgccaaggg
     2701 tagagatcct aatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac
     2761 cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
     2821 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     2881 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2941 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3001 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     3061 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     3121 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     3181 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     3241 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
     3301 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     3361 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     3421 ggccagtgaa ttgtaatacg actcactata gggggccagc gtggccgttt aaacatttaa
     3481 atggccggcc gctgccaccg ttgtttccac cgaagaaacc accgttgccg taaccaccac
     3541 gacggttgtt gctaaagaag ctgccaccgc cacggccacc gttgtagccg ccgttgttgt
     3601 tattgtagtt gctactgtta tttctggcac ttcttggttt tcctcttaag tgaggaggaa
     3661 cataaccatt ctcgttgttg tcgttgatgc ttaaattttg cacttgttcg ctcagttcag
     3721 ccataatatg aaatgctttt cttgttgttc ttacggaata ccacttgcca cctatcacca
     3781 caactaactt tttcccgttc ctccatctct tttatatttt ttttctggag ttcaagagaa
     3841 aaaaaaagaa aaagcaaaaa gaaaaaagga aagcgcgcct cgttcagaat gacacgtata
     3901 gaatgatgca ttaccttgtc atcttcagta tcatactgtt cgtatacata cttactgaca
     3961 ttcataggta tacatatata cacatgtata tatatcgtat gctgcagctt taaataatcg
     4021 gtgtcaagat ctgagctcca ccgcggtggc ggccgcttat ttactcagga tcccgcatgt
     4081 tgcaggtggg agttccagct cccgggaaac agctgcaaga attactcgtg agtaaggaaa
     4141 gagtgaggaa ctatcgcata cctgcattta aagatgccga tttgggcgcg aatcctttat
     4201 tttggcttca ccctcatact attatcaggg ccagaaaaag gaagtgtttc cctccttctt
     4261 gaattgatgt taccctcata aagcacgtgg cctcttatcg agaaagaaat taccgtcgct
     4321 cgtgatttgt ttgcaaaaag aacaaaactg aaaaaaccca gacacgctcg acttcctgtc
     4381 ttcctattga ttgcagcttc caatttcgtc acacaacaag gtcctagcga cggctcacag
     4441 gttttgtaac aagcaatcga aggttctgga atggcgggaa agggtttagt accacatgct
     4501 atgatgccca ctgtgatctc cagagcaaag ttcgttcgat cgtactgtta ctctctctct
     4561 ttcaaacaga attgtccgaa tcgtgtgaca acaacagcct gttctcacac actcttttct
     4621 tctaaccaag ggggtggttt agtttagtag aacctcgtga aacttacatt tacatatata
     4681 taaacttgca taaattggtc aatgcaagaa atacatattt ggtcttttct aattcgtagt
     4741 ttttcaagtt cttagatgct ttctttttct cttttttaca gatcatcaag gaagtaatta
     4801 tctacttttt acaacaaata taaaacaccg cggtggcggc cgctaaaatc atggcctcaa
     4861 acgattatac ccaacaagca acccaaagct atggggccta ccccacccag cccgggcagg
     4921 gctattccca gcagagcagt cagccctacg gacagcagag ttacagtggt tatagccagt
     4981 ccacggacac ttcaggctat ggccagagca gctattcttc ttatggccag agccagaaca
     5041 caggctatgg aactcagtca actccccagg gatatggctc gactggcggc tatggcagta
     5101 gccagagctc ccaatcgtct tacgggcagc agtcctccta tcctggctat ggccagcagc
     5161 cagctcccag cagcacctcg ggaagttacg gtagcagttc tcagagcagc agctatgggc
     5221 agccccagag tgggagctac agccagcagc ctagctatgg tggacagcag caaagctatg
     5281 gacagcagca aagctataat ccccctcagg gctatggaca gcagaaccag tacaacagca
     5341 gcagtggtgg tggaggtgga ggtggaggtg gaggtaacta tggccaagat caatcctcca
     5401 tgagtagtgg tggtggcagt ggtggcggtt atggcaatca agaccagagt ggtggaggtg
     5461 gcagcggtgg ctatggacag caggaccgtg gaggtggagg aatggtgagc aagggcgagg
     5521 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     5581 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     5641 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     5701 acggcctgat gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     5761 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     5821 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5881 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     5941 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     6001 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     6061 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     6121 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     6181 ccgccgggat cactctcggc atggacgagc tgtacaaggc ggccacgcgt atgagcaatt
     6241 cagttttgtc cacttcttcc ccaggtaata tccaaactcg gcaggcgatt ccggcccaac
     6301 tcctctattc cctcgtcaac caaaaagcca gcggcaaact tgtggtacaa aacccctttg
     6361 acgaatttgt ttattggcaa gtttatttgg gggatggtcg tattcacttg gccaacagcg
     6421 ccaatggttg ttccgaaaga cttaattatc tgattggtag cacccttcgt cagaagggca
     6481 ttgaactgcc atcaagaatt gctgacgatt atatttttct ttccgggctc tggaaaaggg
     6541 atgttttttc ctttcagcaa acccgctcga ttctgaccca agctagccaa gaagccttag
     6601 ttcaggtact ttccttacct aaaacatcct atcgttttaa tcaagaaaaa gaactgagcg
     6661 aactattttt gaatttagag ttagaaaaaa ctattacccc catcaaacat aaaattcgct
     6721 attggtggga attgaggtcc tatattaact cgcctttcca gcgaccccta gtggaaaact
     6781 ggcttagtct gcgccaaaat ttggtgaaaa ataacctcca tggccaacat tggctaaaac
     6841 gcttccacac ttgcttggaa aatctaaatt gcttatatga aattgccggg catttggaaa
     6901 tcagtacttt ggagttggca ttaatgctac agcccatggt caagactggc gaaatcaaga
     6961 tgctgcctta tcaagaaatt caaacggatg accgcccttt ggtggcttat gttgaccata
     7021 gggaagtcca gcagcgcatg atgacctatt ctttggaaaa agaaggctat cgagccttag
     7081 tactggatga tccctttcgg gctttaacgg tgttgttgaa ttctcccccc aatctgatta
     7141 tcataaatac tgacttgccg gacatgaacg gttatcaact ctgttcgttt tgccgtaagt
     7201 ccaatcaact taaagatgtg ccgatcctac tattggggaa aggtgacaat ttgatcagcg
     7261 gtattcgtat caaactttgt caagcttctg ggtacttaag tgagcctttt gttccagaag
     7321 aattactcga tcgggtggca aaactccttc ccaccaataa aaccaactcc tgagtcgacc
     7381 tcgagtaatg atcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc
     7441 cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt
     7501 tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta
     7561 cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg
     7621 ctcgaaggct ttaatttgcg gccttatcgc cggcgaataa attaaggcgc gccaacatat
     7681 ccggaggtac ccaaagccga atccaccacg gtcaccgttg tttctgaaga aaccaccacg
     7741 gccgccaccg ttgttgaaac caccacgagg agcagaatcg cctcttctgc caccaccgtt
     7801 acggatgtga ggtggaacgt aaccaccacg tctttgttgc ccttcaggca tggaaacttg
     7861 ctctgctaac tcagtcatat tggatatatg tattactttc tttatttttt gtagtttagt
     7921 cttctatttc ttttatagtt tggtatcttt ttgcaaaaaa tttacctcta tatcgtgttc
     7981 gtgcgtattt cttcctctat aatatgaaaa gaaaggcagt agtcttggtt ttctattatt
     8041 ccaaatatta caagaatacc cagaaccaca agaagcagaa gaaactaact gctttatata
     8101 cttaatttcc cgccacaagc tcatcgccca gaagtttttt tcagtttttc acctgacaat
     8161 ctggcagctc gctgaaaaat tttcacctgg ggtcgcctgc tacgtagagt gatcacgttt
     8221 atgaatagca cattatatag tggcccagac ggtctgatgg tagttcatgt agttaagaat
     8281 aaatactctg tttgtgtgat ttctatgtgt acgttatcta tataaatatt gttgatggtg
     8341 gtagctgtgg gctgtgttct atgctaggac acccttagtg gatggaacgt catcgcgacc
     8401 cgtaggtgtt cttgcctctc tgatggcgat ggcgagcgcc ttgaaagctg attcggagcg
     8461 gtggtggtcg ttggtgcccc gcagacagtc cacatgcatg gtgatacgcg cctcagtggc
     8521 gaaactctcc aagaagtgcg ggatcatctc tgtggatagc tggcctatgc gttctcgctt
     8581 caggcccagc tccaccacgg cgaaaggcct attggagaag tcaaccacag cgcggctcag
     8641 cgcctcgtcc agtggtgcaa acccatgccc gaacctcttg ataccacgca cggagccaag
     8701 cgcctccttg aacgcttggc ccagcgcaat cccacagtcc tcaacagtgt ggtggtcatc
     8761 gatgtgcaag tcccctatac actccaggat cagagaccac ccgccatgct tcgccagcgc
     8821 agtcaacatg tggtccaaaa acccaactcc agtgtgcaca tggatggtct gcccatcacc
     8881 actagcatat tccttgccca gtatgctctc ccgtacagaa acagacccac cgtcaagatc
     8941 cagcgccagc tgtatattcg tctcctgcgt aaccctctta acaaacgcca tcctttgctc
     9001 gatgcttctc tttgcttcaa ttatctcaac tttaagtaaa cctgaagtaa actagaacta
     9061 gattaaatca gaaggcatcc gcagctttat atacatatag agcctctttg ccttcgttta
     9121 tcttgcctgc tcatttttta gtatattctt cgaagaaatc acattacttt atataatgta
     9181 taattcatta tgtgataatg ccaatcgcta agaaaaaaaa agagtcatcc gctaggtgga
     9241 aaaaaaaaaa tgaaaatcat taccgaggca taaaaaaata tagagtgtac tagaggaggc
     9301 caagagtaat agaaaaagaa aattgcggga aaggactgtg ttatgacttc cctgactaat
     9361 gccgtgttca aacgatacct ggcagtgact cctagcgctc accaagctct taaaacgaga
     9421 attaagaaaa agtcgtcatc tttcgataag tttttcccac agcaaagcaa tagtagaaaa
     9481 aaacaatggg aaacgttgaa tgaagacaaa gcgtcgtggt ttaaaaggaa atacgctcac
     9541 gtacatgcta gggaacagga ccgtgcagcg gccagcgtgg ccgtttaaac atttaaatgg
     9601 ccggcc
//
