LOCUS       sequence-253039-        9745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repressilator 2.0, without degradation tags, integrated triple 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Riglar DT, Richmond DL, Potvin-Trottier L, Verdegaal AA, Naydich AD,
            Bakshi S, Leoncini E, Lyon LG, Paulsson J, Silver PA
  TITLE     Bacterial variability in the mammalian gut captured by a single-cell
            synthetic oscillator.
  JOURNAL   Nat Commun. 2019 Oct 11;10(1):4665. doi: 10.1038/s41467-019-12638-z.
  PUBMED    31604953
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-11"; volume: "10(1):4665. doi"; pages: " 
            10.1038/s41467-019-12638-z"
COMMENT     Sequence Label: pLPT234
FEATURES             Location/Qualifiers
     source          1..9745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             22..33
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             40..663
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(113..132)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      690..776
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             883..894
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             912..1994
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(931..950)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      2021..2107
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        join(2138..2156,2157..2162,2163..2179,2180..2185,
                     2186..2211)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    2138..2156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2163..2181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2221..2232
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2239..2952
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     terminator      3010..3096
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3634..4584)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4632..4854
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5346..5440)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5470..6261,6262..6330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6093..6112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6331..6435)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(6574..6660)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6690..7403)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             7410..7421
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    7463..7479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7486..7502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7749..7760
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(8570..8587)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8570..8586)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8605..8691)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(8752..9465)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(8752..9465)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             9474..9485
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(9495..9520,9521..9526,9527..9543,9544..9549,
                     9550..9568))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    9525..9543
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9550..9568
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9686..9702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    9709..9725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gacgcactga ccgaattcat taaagaggag aaaggtacca tgtccagatt agataaaagt
       61 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
      121 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
      181 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
      241 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
      301 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
      361 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
      421 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
      481 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
      541 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
      601 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtct
      661 taagctttag cagcttaatc tagaggcatc aaataaaact aaaggctcag tcgaaagact
      721 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
      781 cgccctagac ctagctgcag gtcgaggata aatatctaac accgtgcgtg ttgactattt
      841 tacctctggc ggtgataatg gttgcatgta ctagaattca ttaaagagga gaaaggtacc
      901 atatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
      961 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     1021 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     1081 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     1141 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     1201 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     1261 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     1321 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccataa
     1381 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     1441 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     1501 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     1561 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     1621 tcgttccaac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     1681 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     1741 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     1801 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     1861 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     1921 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1981 aaagcgggca gtaagcttaa ttagctgagt ctagaggcat caaataaaac gaaaggctca
     2041 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     2101 gacaaatccg ccgccctaga cctagctgca ggtcgagtcc ctatcagtga tagagattga
     2161 catccctatc agtgatagag atactgagca catcagcagg acgcactgac cgaattcatt
     2221 aaagaggaga aaggtaccat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
     2281 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
     2341 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
     2401 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
     2461 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
     2521 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
     2581 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
     2641 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
     2701 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
     2761 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
     2821 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
     2881 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
     2941 acgtttggct gaaaactacg ctttagcagc ttgatctaga ggatcccatg gtacgcgtgc
     3001 tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     3061 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac ctagggtacg
     3121 ggttttgctg cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga
     3181 tccggcttca gccggtttgc cggctgaaag cgctatttct tccagaattg ccatgatttt
     3241 ttccccacgg gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagcagc
     3301 atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt
     3361 tcaatttcat gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca
     3421 tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa cagctttgaa tgcaccaaaa
     3481 actcgtaaaa gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac
     3541 agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat
     3601 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat
     3661 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata
     3721 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt
     3781 taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt
     3841 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc
     3901 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc
     3961 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca
     4021 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc
     4081 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa
     4141 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata
     4201 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc
     4261 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac
     4321 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac
     4381 accataagca ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga
     4441 taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca
     4501 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc
     4561 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata
     4621 ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat
     4681 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa
     4741 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat
     4801 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact
     4861 ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa
     4921 cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa
     4981 tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt
     5041 tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat
     5101 ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc
     5161 gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc
     5221 cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct
     5281 aatgcaccca gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctagtg
     5341 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     5401 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgtaaacttg
     5461 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5521 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5581 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5641 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5701 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5761 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5821 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5881 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5941 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6001 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6061 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6121 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6181 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6241 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6301 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6361 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6421 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctctt tcctgcgtta
     6481 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6541 agcccagcct acaccccggg tctagggcgg cggatttgtc ctactcagga gagcgttcac
     6601 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
     6661 atgcctctag actcagctaa ttaagcttat ttgtatagtt catccatgcc atgtgtaatc
     6721 ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt gggatctttc
     6781 gaaagcttag attgtgtgga caggtaatgg ttgtctggta aaaggacagg gccatcgcca
     6841 attggagtat tttgttgata atggtctgct agttgaacgc ctccatcttc aatgttgtgt
     6901 ctaattttga agttagcttt gattccattc ttttgtttgt ctgccgtgat gtatacattg
     6961 tctgagatgt agttgtattc caatttgtgt ccaagaatgt ttccatcttc tttaaaatca
     7021 atacctttta actcgattct attaacaagg gtatcacctt caaacttgac ttcagcacgt
     7081 gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt cctgtacata accttcgggc
     7141 atggcactct tgaaaaagtc atgctgtttc atatgatctg ggtatcttgc aaagcattga
     7201 acaccccaag tgagagtagt gacaagtgtt ggccatggaa caggtagttt tccagtagtg
     7261 caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca
     7321 gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac aactccagtg
     7381 aaaagttctt ctcctttact catggtacct ttctcctctt taatgaattc ggtcagtgcg
     7441 tcctgctgat gtgctcagta tcttgttatc cgctcacaat gtcaattgtt atccgctcac
     7501 aattctcgag gtgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     7561 tgataataat ggtttcttag acgtcaggtg gcacttttcg ggtcacaaaa gacattccac
     7621 caaacgtcgt ggcggctggc gttccttgga taaatatcta acaccgtgcg tgttgactat
     7681 tttacctctg gcggtgataa tggttgcatg tactagaata tcagcaggac gcactgaccg
     7741 aattcattaa agaggagaaa ggtaccgcat ggttagtaaa ggagaagaaa ataacatggc
     7801 actgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
     7861 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     7921 caaggccgtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
     7981 gtacggcagc aaaaccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc
     8041 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
     8101 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     8161 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctc
     8221 caccgagacc ctgtaccccg ctgacggcgg ccttgaaggc agagccgaca tggccctgaa
     8281 gctcgtgggc gggggccacc tgatctgcaa cttgaagacc acatacagat ccaagaaacc
     8341 cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac agaagactgg aaagaatcaa
     8401 ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
     8461 cctccctagc aaactggggc acagagtcta ggggatcccc cgggctgcag gaattcgata
     8521 tcacgcaaaa aaccccgctt cggcggggtt ttttcgcgag ctcgaattca ctggccgtcg
     8581 ttttacatag gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     8641 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctcta
     8701 gcacgcgtac aatgggatcc cccgggctgc aggaattcga tatcaagctt atttgtatag
     8761 ttcatccatg ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga ccatgtggtc
     8821 tctcttttcg ttgggatctt tcgaaagctt agattggtag gacaggtaat ggttgtctgg
     8881 taaaaggaca gggccatcgc caattggagt attttgttga taatggtctg ctagttgaac
     8941 acctccatct tcaatgttgt gtctaatttt gaagttcgct ttgattccat tcttttgttt
     9001 gtctgccgtg atgtatacat tgtgtgagtt atagttgtat tccaatttgt gtccaagaat
     9061 gtttccatct tctttaaaat caataccttt taactcgatt ctattaacaa gggtatcacc
     9121 ttcaaacttg acttcagcac gtgtcttgta gttcccgtca tctttgaaaa atatagttct
     9181 ttcctgtaca taaccttcgg gcatggcact cttgaaaaag tcatgctgtt tcatatgatc
     9241 tgggtatctc gcaaagcatt gaacaccata acccaaagta gtgacaagtg ttggccatgg
     9301 aacaggtagt tttccagtag tgcaaatcaa tttaagggta agttttccgt atgttgcatc
     9361 accttcaccc tctccactga cagaaaattt gtgcccatta acatcaccat ctaattcaac
     9421 aagaattggg acaactccag tgaaaagttc ttctccttta ctcatgcggt acctttctcc
     9481 tctttaatga attcggtcag tgcgtcctgc tgatgtgctc agtatctcta tcactgatag
     9541 ggatgtcaat ctctatcact gatagggact cgaggtgaag acgaaagggc ctcgtgctcg
     9601 agtacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9661 ggccctttcg tcttcacctc gagaattgtg agcggataac aattgacatt gtgagcggat
     9721 aacaagatac tgagcacatc agcag
//
