LOCUS       sequence-25310-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Hageman J, Kampinga HH
  TITLE     Computational analysis of the human HSPH/HSPA/DNAJ family and 
            cloning of a human HSPH/HSPA/DNAJ expression library.
  JOURNAL   Cell Stress Chaperones. 2009 Jan;14(1):1-21. doi: 
            10.1007/s12192-008-0060-2. Epub 2008 Aug 7.
  PUBMED    18686016
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12192-008-0060-2"; journalName: "Cell Stress Chaperones"; 
            date: "2009-01"; volume: "14"; issue: "1"; pages: "1-21"
COMMENT     Sequence Label: pcDNA5/FRT/TO V5 DNAJB10
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..124
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     RBS             271..279
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctgttttgnc ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa
       61 acttaagctt gaattcacca tgggtaagcc tatccctaac cctctcctcg gtctcgattc
      121 tacgggatcc accatggcat cctactacga gattctagac gtaccgcgga gtgcgttccc
      181 tgatgacatc aagaaggcgt accgaaagaa ggctctgcag tggcacccag acaagaaccc
      241 ggataataaa gaatttgctg aaaaaaaatt taaggaggtg gcagaggcct atgaagtact
      301 atctgacaag cacaaacggg agatctatga ccgctatggc cgggaagggc tgaccggggc
      361 aggaagtggt ccttctcgat cggaaactgg tggtgcgggg cctggcttca cattcacctt
      421 ccgtagcccc gaggaagtct tccgggagtt cttcgggagc ggagaccctt tttcagagct
      481 ctttgatgac ttgggtgtct tctcggagct tcagaaccag ggtccccgac tcacgggccc
      541 tttcttcact ttctcttctt cctttcctgc caactccgat ttctcctcct catctttctc
      601 cttcagcccg ggggctggtg ctttccgctc cgtttctacn tcnncncnnt tnnnnnngnn
      661 nncnnncncn cncnnnnnnn nnannnangg ananngnnag gannnggtan aantnnnnnn
      721 nnnganantg antcnnngnc atcangnnnc cnnangactg nnctngntgn nnnnnnncnt
      781 nnngancagc annnnnnntt nnccnngg
//
