LOCUS       sequence-253170-        2142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC12A8
FEATURES             Location/Qualifiers
     source          1..2142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgacccaga tgtcccaggt gcaggagctg ttccacgagg cagcacagca ggacgcactg
       61 gcacagccac agccctggtg gaagacacag ctgttcatgt gggagcccgt gctgtttggc
      121 acctgggatg gcgtgttcac aagctgcatg atcaacatct tcggcgtggt gctgtttctg
      181 agaaccggct ggctggtggg caatacaggc gtgctgctgg gcatgttcct ggtgtccttt
      241 gtgatcctgg tggccctggt gaccgtgctg tctggcatcg gcgtgggaga gaggagctcc
      301 atcggaagcg gaggcgtgta ctccatgatc tctagcgtgc tgggaggaca gaccggaggc
      361 acaatcggcc tgctgtacgt gtttggccag tgcgtggccg gcgccatgta tatcacaggc
      421 ttcgccgaga gcatctccga cctgctggga ctgggaaaca tctgggccgt gcgcggaatc
      481 tccgtggccg tgctgctggc cctgctgggc atcaatctgg ccggcgtgaa gtggatcatc
      541 cggctgcagc tgctgctgct gtttctgctg gccgtgagca ccctggactt cgtggtgggc
      601 tcctttacac acctggatcc agagcacggc ttcatcggct acagccccga gctgctgcag
      661 aacaataccc tgcctgacta ttccccaggc gagtctttct ttaccgtgtt tggcgtgttc
      721 tttccagcag caacaggcgt gatggcaggc ttcaacatgg gaggcgatct gagggagcca
      781 gcagcatcta tcccactggg aagcctggca gcagtgggaa tctcctggtt tctgtacatc
      841 atcttcgtgt ttctgctggg agcaatctgc acaagggagg ccctgagata tgacttcctg
      901 atcgccgaga aggtgtctct gatgggcttc ctgtttctgc tgggcctgta catctcctct
      961 ctggccagct gtatgggagg actgtatgga gcaccaagga tcctgcagtg catcgcacag
     1021 gagaaagtga tccccgccct ggcatgtctg ggacagggca agggccccaa taagacccct
     1081 gtggcagcaa tctgcctgac cagcctggtg acaatggcct tcgtgtttgt gggccaggtg
     1141 aacgtgctgg cccctatcgt gaccatcaac ttcatgctga catacgtggc cgtggattac
     1201 tcctatttct ctctgagcat gtgctcctgt tctctgacac ctgtgccaga gcccgtgctg
     1261 agagagggag cagagggact gcactgttct gagcacctgc tgctggagaa ggcacctagc
     1321 tatggatccg agggaccagc acagcgggtg ctggagggca ccctgctgga gtttacaaag
     1381 gacatggatc agctgctgca gctgaccaga aagctggaga gctcccagcc taggcaggga
     1441 gagggaaaca ggacaccaga gtcccagaag aggaagtcta agaaggccac caagcagaca
     1501 ctgcaggaca gctttctgct ggatctgaag tctcccccta gcttcccagt ggagatctcc
     1561 gacaggctgc ctgcagcaag ctgggagggc caggagtcct gctggaacaa gcagaccagc
     1621 aagtccgagg gaacccagcc agagggaaca tacggagagc agctggtgcc tgagctgtgc
     1681 aatcagtctg agtctagcgg cgaggacttc tttctgaaga gcaggctgca ggagcaggac
     1741 gtgtggcgga gatctaccag cttttacaca cacatgtgca atccttgggt gtctctgctg
     1801 ggagcagtgg gaagcctgct gatcatgttc gtgatccagt gggtgtatac cctggtgaac
     1861 atgggcgtgg ccgccatcgt gtacttttat atcggcagag catccccagg actgcacctg
     1921 ggatccgcct ctaatttctc cttctttcgg tggatgagat ctctgctgct gccatcctgt
     1981 aggtctctgc gcagccccca ggagcagatc atcctggccc ctagcctggc caaggtggat
     2041 atggagatga cccagctgac acaggagaac gccgacttcg ccaccaggga tcgctaccac
     2101 cactcctctc tggtgaatag ggagcagctg atgccccact at
//
