LOCUS       sequence-253370-        1575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC18A1
FEATURES             Location/Qualifiers
     source          1..1575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             162..170
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgctgagga ccatcctgga cgcaccacag agactgctga aggagggaag ggcaagcagg
       61 cagctggtgc tggtggtggt gttcgtggcc ctgctgctgg acaatatgct gtttaccgtg
      121 gtggtgccaa tcgtgcccac attcctgtac gatatggagt ttaaggaggt gaacagctcc
      181 ctgcacctgg gacacgcagg atctagccca cacgcactgg catctcctgc cttcagcacc
      241 atcttttcct tctttaacaa taacacagtg gcagtggagg agagcgtgcc atccggaatc
      301 gcctggatga atgataccgc atccacaatc ccacctccag caaccgaggc aatctctgcc
      361 cacaagaata actgcctgca gggaaccggc ttcctggagg aggagatcac acgggtgggc
      421 gtgctgtttg ccagcaaggc cgtgatgcag ctgctggtga atcccttcgt gggccctctg
      481 acaaacagaa tcggctacca catccccatg ttcgccggct ttgtgatcat gtttctgtcc
      541 accgtgatgt tcgccttttc tggcacctat acactgctgt tcgtggcacg gacactgcag
      601 ggaatcggat cctcttttag ctccgtggca ggactgggaa tgctggcatc cgtgtacacc
      661 gacgatcacg agcggggcag agccatggga acagccctgg gaggactggc cctgggcctg
      721 ctggtgggcg cccctttcgg cagcgtgatg tatgagtttg tgggcaagtc cgccccattc
      781 ctgatcctgg cctttctggc cctgctggac ggcgccctgc agctgtgcat cctgcagcca
      841 tctaaggtga gcccagagtc cgccaaggga accccactgt tcatgctgct gaaggatccc
      901 tacatcctgg tggccgccgg ctctatctgc tttgccaata tgggagtggc aatcctggag
      961 cctaccctgc caatctggat gatgcagaca atgtgcagcc ctaagtggca gctgggactg
     1021 gccttcctgc cagcatccgt gtcttatctg atcggcacca atctgtttgg cgtgctggcc
     1081 aacaagatgg gcaggtggct gtgctctctg atcggcatgc tggtggtggg cacaagcctg
     1141 ctgtgcgtgc cactggccca caatatcttc ggactgatcg gacctaacgc aggactggga
     1201 ctggcaatcg gaatggtgga ctctagcatg atgcctatca tgggccacct ggtggatctg
     1261 cgccacacca gcgtgtacgg cagcgtgtat gccatcgccg acgtggcctt ctgcatgggc
     1321 tttgcaatcg gaccatccac cggaggagca atcgtgaagg caatcggctt cccctggctg
     1381 atggtcatca caggcgtgat caacatcgtg tacgcccccc tgtgctacta tctgaggagc
     1441 ccccctgcca aggaggagaa gctggccatc ctgtcccagg actgtcctat ggagacacgc
     1501 atgtatgcca cccagaagcc tacaaaggag tttccactgg gcgaggactc tgatgaggag
     1561 cccgatcacg aggag
//
