LOCUS       sequence-253514-        1620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Polesel M, Ingles-Prieto A, Christodoulaki E, Ferrada E, Doucerain 
            C, Altermatt P, Knecht M, Kuhn M, Steck AL, Wilhelm M, Manolova V
  TITLE     Functional characterization of SLC39 family members ZIP5 and ZIP10 
            in overexpressing HEK293 cells reveals selective copper transport 
            activity.
  JOURNAL   Biometals. 2022 Dec 1. doi: 10.1007/s10534-022-00474-6.
  PUBMED    36454509
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biometals. 
            2022 Dec 1. doi: 10.1007/s10534-022-00474-6."
COMMENT     Sequence Label: pDONR221_SLC39A5
FEATURES             Location/Qualifiers
     source          1..1620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgatgggat ctcccgtgag ccacctgctg gcaggattct gcgtgtgggt ggtgctggga
       61 tgggtgggag gatccgtgcc aaatctggga cctgcagagc aggagcagaa ccactacctg
      121 gcccagctgt ttggcctgta tggagagaat ggcaccctga cagcaggagg actggcaagg
      181 ctgctgcact ctctgggcct gggccgggtg cagggcctga gactgggcca gcacggacca
      241 ctgaccggaa gggcagcatc ccctgccgcc gataattcta cacaccgccc ccagaaccct
      301 gagctgagcg tggacgtgtg ggcaggaatg ccactgggac caagcggctg gggcgatctg
      361 gaggagtcca aggcacctca cctgccaagg ggaccagcac ccagcggcct ggacctgctg
      421 cacagactgc tgctgctgga ccactccctg gccgatcacc tgaacgagga ctgcctgaat
      481 ggctcccagc tgctggtgaa cttcggactg tctccagcag cacctctgac cccaaggcag
      541 tttgccctgc tgtgccctgc cctgctgtac cagatcgaca gccgggtgtg catcggagca
      601 cctgcaccag caccacctgg cgatctgctg tctgccctgc tgcagagcgc cctggccgtg
      661 ctgctgctgt ccctgccatc tcctctgagc ctgctgctgc tgaggctgct gggacctagg
      721 ctgctgaggc cactgctggg cttcctgggc gccctggcag tgggcaccct gtgcggcgac
      781 gccctgctgc acctgctgcc tcacgcacag gagggcagac acgcaggacc aggaggactg
      841 cccgagaagg atctgggacc aggactgtcc gtgctgggag gactgttcct gctgtttgtg
      901 ctggagaata tgctgggact gctgaggcac aggggactga ggcccagatg ctgtcggaga
      961 aagaggcgca acctggagac ccggaatctg gacccagaga acggctctgg catggccctg
     1021 cagccactgc aggcagcacc agagccagga gcacagggac agagagagaa gaattctcag
     1081 cacccacccg ccctggcacc tccaggacac cagggacaca gccacggcca ccagggcggc
     1141 accgatatca catggatggt gctgctgggc gatggcctgc acaacctgac agacggactg
     1201 gcaatcggag cagccttctc tgacggcttt agctccggcc tgagcaccac actggccgtg
     1261 ttttgtcacg agctgccaca cgagctgggc gatttcgcca tgctgctgca gtccggcctg
     1321 tcttttcgga gactgctgct gctgagcctg gtgtccggcg ccctgggact gggaggagcc
     1381 gtgctgggcg tgggactgtc cctgggacct gtgccactga ccccatgggt gttcggagtg
     1441 acagcaggcg tgtttctgta tgtggccctg gtggatatgc tgcctgccct gctgaggcca
     1501 cctgagcccc tgcctacccc acacgtgctg ctgcagggac tgggactgct gctgggagga
     1561 ggactgatgc tggcaatcac actgctggag gagcgcctgc tgccagtgac cacagagggc
//
