LOCUS       sequence-253720-        1860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Sijben HJ, van den Berg JJE, Broekhuis JD, IJzerman AP, Heitman LH
  TITLE     A study of the dopamine transporter using the TRACT assay, a novel 
            in vitro tool for solute carrier drug discovery.
  JOURNAL   Sci Rep. 2021 Jan 14;11(1):1312. doi: 10.1038/s41598-020-79218-w.
  PUBMED    33446713
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-01-14"; volume: "11(1):1312. doi"; pages: " 
            10.1038/s41598-020-79218-w"
COMMENT     Sequence Label: pDONR221_SLC6A3
FEATURES             Location/Qualifiers
     source          1..1860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagcaagt ccaagtgcag cgtgggactg atgagctccg tggtggcacc tgcaaaggag
       61 ccaaacgcag tgggccccaa ggaggtggag ctgatcctgg tgaaggagca gaacggcgtg
      121 cagctgacct ctagcaccct gacaaatccc aggcagtctc ctgtggaggc acaggacagg
      181 gagacatggg gcaagaagat cgatttcctg ctgagcgtga tcggctttgc cgtggacctg
      241 gccaacgtgt ggaggttccc atacctgtgc tataagaatg gaggaggagc cttcctggtg
      301 ccatacctgc tgttcatggt catcgccggc atgcccctgt tctatatgga gctggccctg
      361 ggacagttta acagggaggg agcagcaggc gtgtggaaga tctgtcctat cctgaagggc
      421 gtgggcttca ccgtgatcct gatctccctg tacgtgggct tcttttataa tgtgatcatc
      481 gcctgggccc tgcactacct gttctcctct tttaccacag agctgccttg gatccactgc
      541 aacaattctt ggaacagccc aaattgtagc gacgcacacc caggcgacag ctccggcgat
      601 tctagcggcc tgaatgatac attcggcacc acaccagccg ccgagtattt tgagaggggc
      661 gtgctgcacc tgcaccagtc ccacggaatc gacgatctgg gaccacctag atggcagctg
      721 accgcatgcc tggtgctggt catcgtgctg ctgtacttct ctctgtggaa gggcgtgaag
      781 acaagcggca aggtggtgtg gatcaccgcc acaatgccat atgtggtgct gaccgccctg
      841 ctgctgaggg gagtgacact gccaggagca atcgacggaa tcagggcata cctgtccgtg
      901 gacttctaca gactgtgcga ggcaagcgtg tggatcgacg cagcaaccca ggtgtgcttc
      961 agcctgggcg tgggctttgg cgtgctgatc gccttctcct cttacaacaa gtttaccaac
     1021 aactgttaca gagatgccat cgtgaccaca tccatcaatt ctctgacaag cttcagctcc
     1081 ggctttgtgg tgttcagctt tctgggctac atggcccaga agcactccgt gccaatcggc
     1141 gacgtggcaa aggatggacc tggactgatc ttcatcatct atcctgaggc catcgccacc
     1201 ctgccactgt ctagcgcctg ggcagtggtg ttctttatca tgctgctgac cctgggaatc
     1261 gacagcgcca tgggaggcat ggagtccgtg atcacaggcc tgatcgatga gtttcagctg
     1321 ctgcaccggc acagagagct gttcaccctg tttatcgtgc tggccacatt cctgctgtcc
     1381 ctgttttgcg tgaccaacgg cggcatctac gtgttcacac tgctggacca ctttgcagca
     1441 ggcacctcca tcctgttcgg cgtgctgatc gaggcaatcg gagtggcatg gttctacgga
     1501 gtgggacagt tttctgacga tatccagcag atgacaggcc agcggccctc tctgtattgg
     1561 agactgtgct ggaagctggt gagcccttgt ttcctgctgt ttgtggtggt ggtgtccatc
     1621 gtgaccttca ggccacccca ctacggcgcc tatatctttc ccgattgggc caatgccctg
     1681 ggctgggtca tcgccacatc ctctatggcc atggtgccta tctacgccgc ctataagttc
     1741 tgttccctgc caggctcttt tagggagaag ctggcctacg ccatcgcccc tgagaaggac
     1801 agggagctgg tggatagggg agaggtgcgc cagttcaccc tgagacactg gctgaaggtg
//
