LOCUS       sequence-253726-        2688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC9A5
FEATURES             Location/Qualifiers
     source          1..2688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1899..1907
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgctgagag ccgccctgtc tctgctggcc ctgccactgg caggagcagc agaggagcct
       61 acccagaagc cagagagccc aggagagcca cctccaggac tggagctgtt cagatggcag
      121 tggcacgagg tggaggcacc atacctggtg gccctgtgga tcctggtggc aagcctggcc
      181 aagatcgtgt ttcacctgtc taggaaggtg accagcctgg tgcctgagtc ctgcctgctg
      241 atcctgctgg gactggtgct gggaggaatc gtgctggccg tggccaagaa ggccgagtat
      301 cagctggagc caggcacatt ctttctgttc ctgctgcccc ctatcgtgct ggactccggc
      361 tactttatgc cctctcgcct gttctttgat aacctgggcg ccatcctgac ctacgcagtg
      421 gttgggactt tgtggaatgc cttcaccaca ggagccgccc tgtggggact gcagcaggca
      481 ggcctggtgg cccctagggt gcaggcagga ctgctggact tcctgctgtt tggcagcctg
      541 atctccgccg tggatccagt ggccgtgctg gccgtgtttg aggaggtgca cgtgaacgag
      601 accctgttca tcatcgtgtt tggcgagagc ctgctgaatg acgccgtgac agtggtgctg
      661 tacaaggtgt gcaacagctt cgtggagatg ggctccgcca atgtgcaggc caccgattat
      721 ctgaagggcg tggcctctct gtttgtggtg agcctgggag gagcagcagt gggactggtg
      781 ttcgcctttc tgctggccct gaccacaaga ttcacaaaga gagtgaggat catcgagcct
      841 ctgctggtgt ttctgctggc ctacgccgcc tatctgaccg ccgagatggc ctctctgagc
      901 gccatcctgg ccgtgacaat gtgcggcctg ggctgtaaga agtacgtgga ggccaacatc
      961 tctcacaaga gcaggaccac agtgaagtat accatgaaga cactggcctc ctgtgccgag
     1021 accgtgatct tcatgctgct gggcatctcc gccgtggaca gctccaagtg ggcatgggat
     1081 tctggcctgg tgctgggcac cctgatcttc atcctgttct ttcgcgccct gggagtggtg
     1141 ctgcagacat gggtgctgaa tcagtttcgg ctggtgccac tggacaagat cgatcaggtg
     1201 gtcatgagct acggaggact gaggggagca gtggccttcg ccctggtcat cctgctggac
     1261 cggaccaagg tgcccgccaa ggattatttt gtggccacca caatcgtggt ggtgttcttt
     1321 accgtgatcg tgcagggcct gacaatcaag cccctggtga agtggctgaa ggtgaagcgg
     1381 tccgagcacc acaagcctac cctgaaccag gagctgcacg agcacacatt cgaccacatc
     1441 ctggcagcag tggaggatgt ggtgggacac cacggctacc actattggcg cgaccggtgg
     1501 gagcagtttg ataagaagta cctgtcccag ctgctgatgc ggagatctgc ctatagaatc
     1561 agggaccaga tctgggacgt gtactatcgc ctgaatatcc gggacgccat cagcttcgtg
     1621 gatcagggag gacacgtgct gtctagcacc ggactgacac tgccttccat gccatctaga
     1681 aacagcgtgg ccgagacctc cgtgacaaat ctgctgaggg agtccggatc tggagcatgc
     1741 ctggacctgc aggtcatcga taccgtgcgc tctggaaggg acagggagga tgccgtgatg
     1801 caccacctgc tgtgcggagg actgtacaag ccaaggcgcc ggtataaggc cagctgttcc
     1861 agacacttca tctctgagga cgcccaggag aggcaggata aggaggtgtt tcagcagaac
     1921 atgaagagaa ggctggagtc cttcaagtct accaagcaca atatctgttt tacaaagtcc
     1981 aagcctagac caaggaagac cggccgccgg aagaaggacg gagtggcaaa cgcagaggca
     2041 acaaatggca agcacagggg actgggattc caggacaccg cagccgtgat cctgacagtg
     2101 gagagcgagg aggaggagga ggagtccgat tcctctgaga ccgagaagga ggacgatgag
     2161 ggcatcatct ttgtggcaag ggccacaagc gaggtgctgc aggagggcaa ggtgtctgga
     2221 agcctggagg tgtgcccatc tcctaggatc atcccaccca gcccaacctg tgccgagaag
     2281 gagctgccat ggaagagcgg acagggcgac ctggccgtgt acgtgagctc cgagaccaca
     2341 aagatcgtgc ctgtggatat gcagacaggc tggaaccagt ccatctctag cctggagagc
     2401 ctggcctccc ctccatgcaa tcaggcccct atcctgacct gtctgccccc tcacccaaga
     2461 ggcacagagg agccccaggt gcctctgcac ctgccaagcg accccaggtc ctctttcgcc
     2521 tttccaccat ccctggcaaa ggcaggccgc tcccggtctg agagctccgc cgacctgcct
     2581 cagcagcagg agctgcagcc actgatgggc cacaaggatc acacccacct gtctccaggc
     2641 accgccacaa gccactggtg tatccagttc aacagaggct ccaggctg
//
