LOCUS       sequence-253754-        1380 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Polesel M, Ingles-Prieto A, Christodoulaki E, Ferrada E, Doucerain 
            C, Altermatt P, Knecht M, Kuhn M, Steck AL, Wilhelm M, Manolova V
  TITLE     Functional characterization of SLC39 family members ZIP5 and ZIP10 
            in overexpressing HEK293 cells reveals selective copper transport 
            activity.
  JOURNAL   Biometals. 2022 Dec 1. doi: 10.1007/s10534-022-00474-6.
  PUBMED    36454509
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biometals. 
            2022 Dec 1. doi: 10.1007/s10534-022-00474-6."
COMMENT     Sequence Label: pDONR221_SLC39A8
FEATURES             Location/Qualifiers
     source          1..1380
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcccctg gccgggccgt ggccggcctg ctgctgctgg cagcagcagg actgggagga
       61 gtggccgagg gcccaggcct ggccttttcc gaggacgtgc tgagcgtgtt cggagcaaac
      121 ctgtccctgt ctgccgcaca gctgcagcac ctgctggagc agatgggagc agcatccaga
      181 gtgggagtgc cagagcctgg ccagctgcac ttcaaccagt gtctgaccgc cgaggagatc
      241 tttagcctgc acggcttctc caatgccacc cagatcacaa gctccaagtt ttctgtgatc
      301 tgccccgccg tgctgcagca gctgaacttc cacccgtgcg aggataggcc gaaacataag
      361 acaagaccta gccactccga agtgtggggc tacggctttc tgtctgtgac catcatcaat
      421 ctggccagcc tgctgggcct gatcctgaca ccactgatca agaagtccta tttccccaag
      481 atcctgacct tctttgtggg cctggccatc ggcacactgt tttctaacgc catcttccag
      541 ctgatccccg aggcctttgg cttcgaccct aaggtggata gctacgtgga gaaggccgtg
      601 gccgtgtttg gcggcttcta tctgctgttc tttttcgaga ggatgctgaa gatgctgctg
      661 aagacctacg gccagaatgg ccacacacac tttggcaacg acaatttcgg cccccaggag
      721 aagacccacc agccaaaggc cctgccagcc atcaacggag tgacctgcta tgccaatcct
      781 gccgtgacag aggccaacgg ccacatccac tttgacaacg tgagcgtggt gtctctgcag
      841 gatggcaaga aggagccttc tagctgcacc tgtctgaagg gcccaaagct gtccgagatc
      901 ggcaccatcg cctggatgat cacactgtgc gacgccctgc acaacttcat cgatggcctg
      961 gccatcggcg cctcttgtac cctgagcctg ctgcagggcc tgtctacaag catcgccatc
     1021 ctgtgcgagg agtttcccca cgagctgggc gacttcgtga tcctgctgaa cgccggcatg
     1081 tccaccagac aggccctgct gtttaatttc ctgagcgcct gctcctgtta cgtgggactg
     1141 gccttcggca tcctggtggg caacaatttt gccccaaata tcatcttcgc cctggccggc
     1201 ggcatgtttc tgtatatcag cctggccgac atgttccccg agatgaacga tatgctgagg
     1261 gagaaggtga ccggccgcaa gaccgacttc accttcttca tgatccagaa tgccggcatg
     1321 ctgaccggct tcacagccat cctgctgatc acactgtacg ccggcgagat cgagctggag
//
