LOCUS       sequence-253772-        2781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC8A3
FEATURES             Location/Qualifiers
     source          1..2781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcctggc tgagactgca gcccctgaca agcgcctttc tgcacttcgg cctggtgacc
       61 tttgtgctgt tcctgaacgg actgagggca gaggcaggag gatccggcga cgtgccttct
      121 accggccaga acaatgagag ctgttccggc agctccgatt gcaaggaggg cgtgatcctg
      181 ccaatctggt accccgagaa tccttccctg ggcgacaaga tcgcccgcgt gatcgtgtac
      241 tttgtggccc tgatctatat gttcctgggc gtgtctatca tcgccgatcg cttcatggcc
      301 agcatcgaag tgatcacatc ccaggagcgg gaggtgacca tcaagaagcc aaacggcgag
      361 acatccacca caaccatccg ggtgtggaac gagacagtgt ctaatctgac cctgatggca
      421 ctgggatcta gcgccccaga gatcctgctg tccctgatcg aggtgtgcgg acacggcttc
      481 atcgcaggcg acctgggacc tagcaccatc gtgggatccg ccgcctttaa tatgttcatc
      541 atcatcggca tctgcgtgta cgtgatcccc gatggcgaga caagaaagat caagcacctg
      601 agggtgttct ttatcaccgc cgcctggtcc atctttgcct acatctggct gtatatgatc
      661 ctggccgtgt tctctcctgg agtggtgcaa gtgtgggagg gactgctgac cctgttcttt
      721 ttccccgtgt gcgtgctgct ggcatgggtg gcagacaagc gcctgctgtt ctacaagtat
      781 atgcacaaga agtatcgcac agataagcac cggggcatca tcatcgagac cgagggcgat
      841 cacccaaagg gcatcgagat ggacggcaag atgatgaaca gccactttct ggatggcaat
      901 ctggtgcccc tggagggcaa ggaggtggac gagtcccgga gagagatgat cagaatcctg
      961 aaggatctga agcagaagca ccccgagaag gacctggatc agctggtgga gatggccaac
     1021 tactatgccc tgtctcacca gcagaagagc agagccttct acaggatcca ggccacaagg
     1081 atgatgaccg gcgccggcaa tatcctgaag aagcacgcag cagagcaggc caagaaggca
     1141 tcctctatga gcgaggtgca cacagatgag cccgaggact ttatctccaa ggtgtttttc
     1201 gacccttgct cttaccagtg tctggagaac tgcggagccg tgctgctgac cgtggtgcgg
     1261 aagggcggcg acatgtctaa gacaatgtac gtggattata agaccgagga cggaagcgcc
     1321 aatgcaggag cagattatga gttcacagag ggaaccgtgg tgctgaagcc aggagagacc
     1381 cagaaggagt tttctgtggg catcatcgac gatgacatct tcgaggagga cgagcacttt
     1441 ttcgtgagac tgagcaacgt gaggatcgag gaggagcagc cagaggaggg aatgccacct
     1501 gccatcttta atagcctgcc tctgccaaga gccgtgctgg catccccttg cgtggcaaca
     1561 gtgaccatcc tggatgacga tcacgccggc atctttacat tcgagtgcga taccatccac
     1621 gtgtctgaga gcatcggcgt gatggaggtg aaggtgctgc gcacaagcgg agcaagggga
     1681 accgtgatcg tgcctttcag gacagtggag ggaaccgcaa agggaggagg agaggatttt
     1741 gaggacacct acggcgagct ggagttcaag aacgacgaga cagtgaagac catccgcgtg
     1801 aagatcgtgg atgaggagga gtatgagcgg caggagaact tcttcatcgc cctgggcgag
     1861 ccaaagtgga tggagagagg catctccgcc ctgctgctgt ctcccgatgt gacagacaga
     1921 aagctgacca tggaggagga ggaggccaag aggatcgccg agatgggcaa gccagtgctg
     1981 ggcgagcacc ccaagctgga agtgatcatc gaggagtcct acgagttcaa gacaaccgtg
     2041 gacaagctga tcaagaagac aaacctggcc ctggtggtgg gaacccacag ctggcgcgat
     2101 cagtttatgg aggcaatcac agtgtccgcc gcaggcgacg aggatgagga cgagtctggc
     2161 gaggagcggc tgcctagctg ttttgactac gtgatgcact ttctgacagt gttctggaag
     2221 gtgctgttcg cctgcgtgcc acccaccgag tattgtcacg gctgggcctg ctttgccgtg
     2281 tctatcctga tcatcggcat gctgaccgcc atcatcggcg acctggcctc tcacttcggc
     2341 tgtacaatcg gcctgaagga tagcgtgacc gccgtggtgt ttgtggcctt cggcacatcc
     2401 gtgccagaca cctttgcctc taaggcagcc gccctgcagg acgtgtacgc agacgccagc
     2461 atcggcaatg tgacaggctc caacgccgtg aacgtgttcc tgggaatcgg actggcatgg
     2521 tccgtggcag ccatctattg ggccctgcag ggccaggagt ttcacgtgtc tgccggcacc
     2581 ctggccttta gcgtgacact gttcaccatc tttgccttcg tgtgcatcag cgtgctgctg
     2641 tacaggcgcc ggcctcacct gggaggagag ctgggaggac caaggggctg caagctggcc
     2701 acaacctggc tgttcgtgag cctgtggctg ctgtatatcc tgttcgccac cctggaggcc
     2761 tactgctata tcaagggctt c
//
