LOCUS       sequence-254065-       14004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating cell line expressing the 2P-activatable RAF system 
            with P2A and full-length RAF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14004)
  AUTHORS   Kinjo T, Terai K, Horita S, Nomura N, Sumiyama K, Togashi K, Iwata 
            S, Matsuda M
  TITLE     FRET-assisted photoactivation of flavoproteins for in vivo 
            two-photon optogenetics.
  JOURNAL   Nat Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 
            10.1038/s41592-019-0541-5.
  PUBMED    31501546
REFERENCE   2  (bases 1 to 14004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Sep 9. pii: 10.1038/s41592-019-0541-5. doi: 
            10.1038/s41592-019-0541-5."
COMMENT     Sequence Label: pT2ADWpuro_2paRAF
FEATURES             Location/Qualifiers
     source          1..14004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5838..5894
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5895..6404
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             6423..7118
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6556..6574)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6824..6843
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7257..7806
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7401..7418)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7628..7647
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7809..8408
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7809..7828)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8305..8325
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8426..9014
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8479..8499)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8897..8908)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9079..9213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9103..9122)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9157..9176
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9335..9497,9498..9509)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9539..9558)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9540..9558)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9568..9585)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9568..9584)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9577..9599)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9726..10180
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9812..9831)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10022..10043
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10206..10310
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10311..10379,10380..11171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10529..10548)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11342..11930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11831..11850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12084..12101
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12218..12239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12254..12259,12260..12277,12278..12284)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     12316..12332
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12316..12332
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12351..12371
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12353..12371
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(12428..12439,12440..12627)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc crgggggggs ggggsggggs grggggsggg
      541 gsggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc accatgaaga
     1741 tggacaagaa aaccatcgtg tggtttcgga gagatctgag gattgaggac aacccagccc
     1801 tggccgctgc cgctcacgaa ggcagcgtgt ttccagtgtt catctggtgc cccgaggaag
     1861 agggacagtt ctacccagga agggccagcc gctggtggat gaagcagtcc ctggctcacc
     1921 tgtctcagag tctgaaggcc ctggggtcag atctgaccct gatcaaaacc cataacacaa
     1981 tcagcgccat tctggactgt attcgggtga ctggagccac caaagtggtg tttaatcacc
     2041 tgtatgatcc cgtgagcctg gtgcgggacc atacagtgaa ggagaaactg gtggaaagag
     2101 gcatcagtgt gcagtcatac aacggggatc tgctgtatga gccatgggaa atctactgcg
     2161 agaaggggaa accctttacc agcttcaatt cctactggaa gaaatgtctg gacggatctg
     2221 gaatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     2281 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     2341 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     2401 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     2461 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     2521 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     2581 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     2641 gggaggcatt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     2701 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc acatatagat
     2761 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     2821 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     2881 gatacggtag cggtccatgg aggctgatgc ctattacagc cgctgccgaa gctatctggg
     2941 cctgctccat tgaagagctg ggactggaaa acgaggccga aaagccttct aatgctctgc
     3001 tgactcgcgc ctggtcccca ggatggtcta acgctgataa actgctgaat gagttcatcg
     3061 aaaagcagct gattgactat gccaaaaact ctaagaaagt ggtgggcaac agcaccagcc
     3121 tgctgtctcc atacctgcac tttggagaga tcagtgtgcg gcacgtgttc cagtgcgcta
     3181 gaatgaagca gatcatttgg gccagggata aaaacagtga gggcgaagag tcagccgacc
     3241 tgtttctgag gggaatcgga ctgagggagt actcccgcta tatttgtttc aattttccct
     3301 tcactcacga acagtcactg ctgagccatc tgcggttctt tccttgggac gctgatgtgg
     3361 acaagttcaa agcctggcgg cagggaagaa caggctatcc cctggtggat gctggaatga
     3421 gagagctgtg ggccactggc tggatgcaca ataggatccg cgtgattgtg agctcctttg
     3481 ctgtgaagtt cctgctgctg ccttggaagt ggggcatgaa atatttttgg gatacactgc
     3541 tggatgccga cctggagtgt gacatcctgg ggtggcagta catctccgga tctattccag
     3601 atggccatga actggatagg ctggacaacc ccgctctgca gggcgctaag tatgaccctg
     3661 agggagaata catcaggcag tggctgcctg agctggctag gctgccaact gaatggattc
     3721 accatccctg ggacgctcct ctgacagtgc tgaaggccag tggggtggag ctgggaacta
     3781 attacgctaa acctatcgtg gatattgaca ccgccagaga gctgctggct aaggccatct
     3841 cccggaccag agaagcccag atcatgattg gcgctgccat ggagcacata cagggagctt
     3901 ggaagacgat cagcaatggt tttggattca aagatgccgt gtttgatggc tccagctgca
     3961 tctctcctac aatagttcag cagtttggct atcagcgccg ggcatcagat gatggcaaac
     4021 tcacagatcc ttctaagaca agcaacacta tccgtgtttt cttgccgaac aagcaaagaa
     4081 cagtggtcaa tgtgcgaaat ggaatgagct tgcatgactg ccttatgaaa gcactcaagg
     4141 tgaggggcct gcaaccagag tgctgtgcag tgttcagact tctccacgaa cacaaaggta
     4201 aaaaagcacg cttagattgg aatactgatg ctgcgtcttt gattggagaa gaacttcaag
     4261 tagatttcct ggatcatgtt cccctcacaa cacacaactt tgctcggaag acgttcctga
     4321 agcttgcctt ctgtgacatc tgtcagaaat tcctgctcaa tggatttcga tgtcagactt
     4381 gtggctacaa atttcatgag cactgtagca ccaaagtacc tactatgtgt gtggactgga
     4441 gtaacatcag acaactctta ttgtttccaa attccactat tggtgatagt ggagtcccag
     4501 cactaccttc tttgactatg cgtcgtatgc gagagtctgt ttccaggatg cctgttagtt
     4561 ctcagcacag atattctaca cctcacgcct tcacctttaa cacctccagt ccctcatctg
     4621 aaggttccct ctcccagagg cagaggtcga catccacacc taatgtccac atggtcagca
     4681 ccaccctgcc tgtggacagc aggatgattg aggatgcaat tcgaagtcac agcgaatcag
     4741 cctcaccttc agccctgtcc agtagcccca acaatctgag cccaacaggc tggtcacagc
     4801 cgaaaacccc cgtgccagca caaagagagc gggcaccagt atctgggacc caggagaaaa
     4861 acaaaattag gcctcgtgga cagagagatt caagctatta ttgggaaata gaagccagtg
     4921 aagtgatgct gtccactcgg attgggtcag gctcttttgg aactgtttat aagggtaaat
     4981 ggcacggaga tgttgcagta aagatcctaa aggttgtcga cccaacccca gagcaattcc
     5041 aggccttcag gaatgaggtg gctgttctgc gcaaaacacg gcatgtgaac attctgcttt
     5101 tcatggggta catgacaaag gacaacctgg caattgtgac ccagtggtgc gagggcagca
     5161 gcctctacaa acacctgcat gtccaggaga ccaagtttca gatgttccag ctaattgaca
     5221 ttgcccggca gacggctcag ggaatggact atttgcatgc aaagaacatc atccatagag
     5281 acatgaaatc caacaatata tttctccatg aaggcttaac agtgaaaatt ggagattttg
     5341 gtttggcaac agtaaagtca cgctggagtg gttctcagca ggttgaacaa cctactggct
     5401 ctgtcctctg gatggcccca gaggtgatcc gaatgcagga taacaaccca ttcagtttcc
     5461 agtcggatgt ctactcctat ggcatcgtat tgtatgaact gatgacgggg gagcttcctt
     5521 attctcacat caacaaccga gatcagatca tcttcatggt gggccgagga tatgcctccc
     5581 cagatcttag taagctatat aagaactgcc ccaaagcaat gaagaggctg gtagctgact
     5641 gtgtgaagaa agtaaaggaa gagaggcctc tttttcccca gatcctgtct tccattgagc
     5701 tgctccaaca ctctctaccg aagatcaacc ggagcgcttc cgagccatcc ttgcatcggg
     5761 cagcccacac tgaggatatc aatgcttgca cgctgaccac gtccccgagg ctgcctgtct
     5821 tcctcgaggg aagcggagcg acgaatttta gtctactgaa acaagcggga gacgtggagg
     5881 aaaaccctgg acctatgaat ggagctatag gaggtgacct tttgctcaat tttcctgaca
     5941 tgtcggtcct agagcgccaa agggctcacc tcaagtacct caatcccacc tttgattctc
     6001 ctctcgccgg cttctttgcc gattcttcaa tgattaccgg cggcgagatg gacagctatc
     6061 tttcgactgc cggtttgaat cttccgatga tgtacggtga gacgacggtg gaaggtgatt
     6121 caagactctc aatttcgccg gaaacgacgc ttgggactgg aaatttcaag gcagcgaagt
     6181 ttgatacaga gactaaggat tgtaatgagg cggcgaagaa gatgacgatg aacagagatg
     6241 acctagtaga agaaggagaa gaagagaagt cgaaaataac agagcaaaac aatgggagca
     6301 caaaaagcat caagaagatg aaacacaaag ccaagaaaga agagaacaat ttctctaatg
     6361 attcatctaa agtgacgaag gaattggaga aaacggatta tattcatctc gagggatctg
     6421 gaatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg
     6481 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     6541 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg
     6601 acatcctgtc ccctcagttc atgtacggct ccagggcctt catcaagcac cccgccgaca
     6661 tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     6721 tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga
     6781 tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga
     6841 agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg
     6901 gcgacattaa gatggccctg cgcctgaagg acggaggccg ctacctggcg gacttcaaga
     6961 ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt
     7021 tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg
     7081 gccgccactc caccggcggc atggacgagc tgtacaagtc tagaaagatg agcaaagatg
     7141 gtaaaaagaa gaaaaagaag tcaaagacaa agtgtgtaat tatgtaatct agagtcaact
     7201 aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt
     7261 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     7321 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     7381 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     7441 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     7501 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     7561 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     7621 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     7681 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7741 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     7801 ataataccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     7861 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7921 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7981 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     8041 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     8101 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     8161 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     8221 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     8281 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     8341 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     8401 gtgcctgagt cgacggatcc tcgataatca acctctggat tacaaaattt gtgaaagatt
     8461 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     8521 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     8581 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     8641 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     8701 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     8761 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     8821 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     8881 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     8941 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     9001 ggccgcctcc ccgcatctcg acaaaggatc caatggaggc cgccaccgcg gtggagctcg
     9061 aattaattca tcgatgatga tccagacatg ataagataca ttgatgagtt tggacaaacc
     9121 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     9181 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     9241 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     9301 ggtatggctg attatgatcc tctagatcag atctttcttg cttttacttt tacttcctta
     9361 atactcaagt acaattttaa tggagtactt ttttactttt actcaagtaa gattctagcc
     9421 agatactttt acttttaatt gagtaaaatt ttccctaagt acttgtactt tcacttgagt
     9481 aaaatttttg agtacttttt acacctctgt caagaaccat atgccggtac ccaattcgcc
     9541 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     9601 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     9661 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     9721 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9781 gaccrccaca cccgccgcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9841 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9901 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9961 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    10021 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    10081 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    10141 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    10201 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10261 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10321 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    10381 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10441 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10501 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    10561 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    10621 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    10681 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10741 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10801 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    10861 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10921 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10981 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11041 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11101 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11161 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11221 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11281 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    11341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    11401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11461 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    11521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    11581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    11641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    11701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    11761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    11821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    11881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    12001 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    12061 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    12121 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    12181 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    12241 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgatcg
    12301 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    12361 ctcactaaag ggaacaaaag ctggagctcg gatctgctgg gcttgctgaa ggtagggggt
    12421 caagaaccag aggtgtaaag tacttgagta attttacttg attactgtac ttaagtatta
    12481 tttttgggga tttttacttt acttgagtac aattaaaaat caatactttt acttttactt
    12541 aattacattt ttttagaaaa aaaagtactt tttactcctt acaattttat ttacagtcaa
    12601 aaagtactta ttttttggag atcacttggg ccctctagat gcatgccggc tggcccaggc
    12661 catcggcatt ccggagccca gggtccagat ttggtttcag aatgagaggt cacgccagct
    12721 gaggcagcac cggcgggaat ctcggccctg gcccgggaga cgcggcccgc cagaaggccg
    12781 gcgaaagcgg accgccgtca ccggatccca gaccgccctg ctcctccgag cctttgagaa
    12841 ggatcgcttt ccaggcatcg ccgcccggga ggagctggcc agagagacgg gcctcccgga
    12901 gtccaggatt cagatctggt ttcagaatcg aagggccagg cacccgggac agggtggcag
    12961 ggcgcccgcg caggcaggcg gcctgtgcag cgcggccccc ggcgggggtc accctgctcc
    13021 ctcgtgggtc gccttcgccc acaccggcgc gtggggaacg gggcttcccg caccccacgt
    13081 gccctgcgcg cctggggctc tcccacaggg ggctttcgtg agccaggcag cgagggccgc
    13141 ccccgcgctg cagcccagcc aggccgcgcc ggcagagggg atctcccaac ctgccccggc
    13201 gcgcggggat ttcgcctacg ccgccccggc tcctccggac ggggcgctct cccaccctca
    13261 ggctcctcgc tggcctccgc acccgggcaa aagccgggag gaccgggacc cgcagcgcga
    13321 cggcctgccg ggcccctgcg cggtggcaca gcctgggccc gctcaagcgg ggccgcaggg
    13381 ccaaggggtg cttgcgccac ccacgtccca ggggagtccg tggtggggct ggggccgggg
    13441 tccccaggtc gccggggcgg cgtgggaacc ccaagccggg gcagctccac ctccccagcc
    13501 cgcgcccccg gacgcctccg cctccgcgcg gcaggggcag atgcaaggca tcccggcgcc
    13561 ctcccaggcg ctccaggagc cggcgccctg gtctgcactc ccctgcggcc tgctgctgga
    13621 tgagctcctg gcgagcccgg agtttctgca gcaggcgcaa cctctcctag aaacggaggc
    13681 cccgggggag ctggaggcct cggaagaggc cgcctcgctg gaagcacccc tcagcgagga
    13741 agaataccgg gctctgctgg aggagcttta ggacgcgggg ttgggacggg gtcgggtggt
    13801 tcggggcagg gccgtggcct ctctttcgcg gggaacacct ggctggctac ggaggggcgt
    13861 gtctccgccc cgccccctcc accgggctga ccggcctggg attcctgcct tctaggtcta
    13921 ggcccggtga gagactccac accgcggaga actgccattc tttcctgggc atcccgggga
    13981 tcccagagcc ggcccagcat gctc
//
